大肠杆菌

The phage antibody was expressed in E.

该噬菌体抗体被有效表达在大肠杆菌体系中。

By adding a sequence encoding a small peptide,〓, to the base sequence of pYZFcd20, a new phasmid pYZcpp3 was constructed. Using the pYZcpp3 to transform E. coli. 16c9, the genetically engineered bacteria 10916# was obtained. 10916# can secret the soluble chimeric antibody fragment anti-CD20 F〓 into periplasmic.

通过对质粒pYZFcd20的碱基序列的改造，在CH1基因的末端添加了一截二聚化结构的编码序列，实现了在大肠杆菌中直接表达可溶性抗CD20嵌和抗体片段F〓的目的。

Inan attempt to select more efficient biocatalysts ,the hydantoinase and carbamylase genes fromBurkholderia pickettii were cloned in Escherichia coli, achieve a co-experssed strains. The geneswere assembled to give the carbamylase gene preceding the hydantoinase gene. The recombinantstrains stably and constitutively produced the two enzymes and efficiently converted thecorresponding hydantoins onto p-hydroxyphenylglycine and phenylglycine .

我们试着寻找更快更有效的转化菌株，把来源于皮氏伯克霍尔德氏菌的表达该两种酶的两个基因同时克隆到大肠杆菌中，获得共表达两种酶的基因工程菌株，两个基因的顺序是 Dcase 基因在 Dhase 基因的前面，同一个启动子。

E. Coli polynucleotide phosphorylase was isolated and purified with a purification factor of 68 times.

本文的第一部分报导了从大肠杆菌中分离多核苷酸■酸化酶的工作。

To study the effects E.coli purine nucleoside phosphorylase suicide gene on human gastric cancer MKN-45 cells as well as the bystander effect.

目的：探讨大肠杆菌嘌呤核苷磷酸化酶自杀基因对胃癌细胞MKN-45的抑制及杀伤作用，并证实旁观者效应。

In this paper, we studied the effect of sterilization on different bacteria and cyanobacteria in variant water sample of micro-electrolysis and high voltage electrostatic field, and the effect of physiological activity on E.coli and the characteristic optical absorption peak of phycocyanin of micro-electrolysis. The mechanism of sterilization and algae killing was approached.

本文研究了微电解、高压静电场作用对于不同水样中的不同种类的细菌和蓝藻的杀灭作用，以及微电解作用对于大肠杆菌的生理活性和对于蓝藻细胞内藻胆蛋白的光吸收特性的影响，探讨了杀菌灭藻的机理。

The expression of phycoerythrin beta and alfa subunit genes in E.coli and its activity study was done and will be done further.

另进行了龙须菜藻红蛋白b和a亚基基因在大肠杆菌体系中的蛋白质表达及蛋白活性研究。

A separated pathogenetic material of edema disease of pigling from endo-toxin is isolated, which is prepared from hemolytic E coli O139:K82 of the natural edema disease cases, by spreading culture, repeatedly freezing and thawing, rapidly centrifugalizing, concentrating and doing sephadexG-200 column chromatography.

本文主要报道从仔猪水肿病自然病例分离的溶血性大肠杆菌O_(130)：K_(82)，通过扩大培养，反复冻融，离心、浓缩，进行SephadexG-200柱层析，分离出了一种与内毒素分开的仔猪水肿病致病物质。

Total RNA was isolated from cultured human pigmental cells and mRNA was reversly transcribed into cDNA. Then PCR was used to amplify the TRP -2 coding region. The PCR product was cloned into pUC19 plasmid and sequenced, then subcloned into vector pGEX-4T-1. The TRP-2 protein was expressed hi E coli of DH5 a as fusion protein with glutathioneS-transferase induced by IPTG. The fusion protein was purified by glutathione resin column.

我们通过RT—PCR的方法扩增TRP—2编码基因，克隆至pUC19载体并测序，再亚克隆至GST融合表达载体pGEX—4T—1，转化大肠杆菌，IPTG诱导表达了GST/TRP—2融合蛋白，并分析鉴定表达蛋白的可溶性，最后第四军医大学硕士学位论文一用谷氨酚胺树脂柱纯化表达的蛋白。

Avian Escherichia coli ; type 1 pili ; structural gene ; cloning

鸡大肠杆菌； 1型菌毛；结构基因；克隆

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。