大肠杆菌

The plasmid of pMD-T-GO and pPIC3.5K were extracted from JM109 strain and then glycolate oxidase gene fragment was cloned into the expression vector (pPIC3.5K). The recombinant plasmids were transformed into E.coli TOP 10Fˊ. The recombinant clones were identified by PCR, digested with SnaB I /Not I and then sequenced.

本文首先从大肠杆菌JM109中提取质粒pMD-T-GO和pPIC3.5K，将菠菜乙醇酸氧化酶基因c DNA片段克隆至表达载体pPIC3.5K，转化进E.coli TOP 10Fˊ，利用设计的引物进行PCR扩增、SnaB I和Not I酶切鉴定，最后进行序列分析。

Gnomic fragments of 4～20 kilobases were obtained by digesting genomic DNA of V.cholerae O 139 with restriction endonuclease Eco RI,then plasmid gene bank was constructed.

随机筛选重组克隆，获得一株可与O1 39霍乱弧菌抗血清发生凝集反应的重组克隆，命名为大肠杆菌DH5α(pMG32 0 )。

The expression vector pET32a-ITF was constructed and expressed in E.coli successfully, and the recombinant protein rITF and its antibody was also purified in this study. ITF expressed in small intestine goblet cell.

成功构建了表达载体pET32a-ITF，在大肠杆菌中表达并纯化获得纯度较高的rITF，并获得了生物活性较高的ITF抗体，ITF主要在肠道杯状细胞分泌表达。

The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

For irradiated individuals the quantity of bacterial challenge-related proteins in hemolymph induced by E, coli significantly decreased.

照射蚕注射大肠杆菌后在体内诱导出现的应急蛋白量明显比对照蚕要少。

Aim To optimize purification conditions of recombinant hirudin 3 in the fermentation broth and characterize the product.

目的优化分离纯化大肠杆菌发酵液中重组水蛭素Ⅲ的工艺并对所纯化产物进行分子鉴定。

The morphological change of Escherichia coli were observed by transmission electron microscope, and the antibacterial mechanism of Fe/Ce codoped nano-TiO2 hydrosol was discussed.

采用透射电镜对杀菌过程中的大肠杆菌菌体形态进行观测，探讨了Fe/Ce共掺杂纳米TiO2水溶胶的抗菌机理。

After six rounds of selection in medium containing mycophenolic acid, xanthine and hypoxanthine, we obtained purified recombinant viruses.

提取purified-rHVT感染细胞总DNA电转化大肠杆菌DH10B感受态细胞，在氯霉素抗性平板上筛选阳性克隆，并用酶切和PCR方法对其进行鉴定。

And starting tomorrow, the government will allow food producers to start zap ping fresh spinach and iceberg lettuce with just enough radiation to kill E.coli and other dangerous germs.

从明天开始，政府允许食品生产者使用足够的射线来杀死新鲜菠菜和卷心莴苣上的大肠杆菌和其它危险的细菌。

And starting tomorrow, the government will allow food producers to start zapping fresh spinach and iceberg lettuce with just enough radiation to kill E.coli and other dangerous germs.

从明天开始，政府将允许食物生产制造商在处理新鲜菠菜和卷心莴苣的时候，使用适当的激光来消灭大肠杆菌和其他有害基因。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。