大肠杆菌

The radical scavenging activity of Laminaria japonica pigments was studied by the DPPH2,2-Diphenyl~(-1-picrylhydrazylassay. The total pigments showed a strong DPPH radical scavenging activity of 65.819% with the concentration of 0.52mg.ml~(-1). The DPPH radical scavenging activity of fucoxanthin was 19.85% with the concentration of 0.27mg.ml~(-1). The antimicrobial activity of pigment in Laminaria japonica was studied by the method of round paper. The results showed that the pigment extracts have activity against Escherichia coli, Pseudomonas aeruginosa, Saricina flava and Saccharomyes cerevisia. The antibacterial activity of total pigments for Saccharomyes cerevisia was the strongest with an inhibition diameter of 18.9 mm; and the antibacterial activity of fucoxanthin for Saricina flava was also much significant with an inhibition diameter of 10.2 mm.

测定了它们对自由基的清除率和对四种细菌的抑菌效果，研究结果表明，总色素和褐藻黄素的浓度与自由基清除率成正比，当总色素浓度为0.52mg.ml~(-1)时，清除率达到65.819%，高于陆生植物黑米色素和紫甘薯色素；当褐藻黄素浓度为0.27mg.ml~(-1)时，自由基清除率达到19.85%，低于紫苏色素、紫甘蓝色素、黑米色素、紫甘薯色素；对大肠杆菌、绿脓杆菌、八叠球菌、金黄葡萄球菌的抑菌实验结果显示，海带总色素对金黄葡萄球菌的抑菌活性最强，抑菌圈直径为18.9mm；褐藻黄素对八叠球菌的抑菌活性相对较强，抑菌圈直径为10.2mm。

To determinant the functional motif of S protein involved in the interaction with ACE2, seven truncated S proteins deleted from N or C terminal were obtained by E.

为了确定S蛋白与ACE2结合的功能区域，我们在大肠杆菌中表达了7个从N端截短或从C端截短的S蛋白，并用Ni柱纯化。

We obtained a strain that increased the level of functional expression of heterologous protein with multiple bonds.

本研究建立了在大肠杆菌染色体上进行基因敲入的方法，观察了共表达及敲入到染色体上的折叠调节因子对外源蛋白功能性表达的影响，获得了对外源蛋白的功能性表达有促进作用的菌株。

After optimizing the expression conditions, functional xylanase XT6 was over expressed in E. coli with up to 65% of total protein.

通过优化表达条件，功能正常的XT6基因在大肠杆菌中成功过量表达，蛋白表达量占细胞中总蛋白的65%。

Coli mainly in a form of inclusion body. The expressed product contained about 25% of total somatic protein and showed good reactogenicity as proved by Western blot. The IgG level against GAMP induced by GAMP-CRM197 conjugate was significantly higher than those by GAMP and by the mixture of CAMP and rCRM197. Repeated immunization with the conjugate induced immunopotentiation, indicating the effect of CRM197 as a carrier protein.

结果重组CRM197在大肠杆菌中主要以包涵体形式表达，表达量占菌体总蛋白的25%左右；Western blot证明重组rCRM197具有良好的反应原性；各针接种后，结合物诱生的多糖特异性IgG水平均显著高于GAMP组和GAMP+rCRM197混合物组，具有较强的免疫原性；多次接种产生了免疫增强效应，重组CRM197具有载体蛋白的作用。

In this paper, a new product of Ce/ TiO2 compound antibacterial agent has been made, comparing with naked nano－TiO2, it has better antimicrobial activity against E.coli ,S.aureus and scorch gemmule bacillus in the solar light, increasing the quantity of Ce4+, exhibiting stronger antibacterial property.

制备出稀土铈掺杂锐钛型纳米TiO2复合抗菌功能材料，该抗菌材料与不掺杂的纳米TiO2相比，在可见光下对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌的混合菌具有较好的抑菌效果，随着Ce4+的摩尔浓度增加，制备的试样的抗菌能力越强。

The above experiments showed that the UHP has an impact on E.coli DH5αgerm plasm.

通过以上实验，说明超高压对大肠杆菌遗传物质产生了影响。

Publication information Written by Francis E. Gigot.

出版信息作者弗朗西斯大肠杆菌吉戈特。

Publication information Written by Frances E. Gigot.

出版信息作者弗朗西斯大肠杆菌吉戈特。

Cloning and expression of gsiB was carried out for further study of the protein structure and function of glutathione transport system.

为深入研究大肠杆菌谷胱甘肽转运系统的蛋白质结构和功能，对该系统中的gsiB基因进行了克隆和表达条件的优化。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。