大肠杆菌

The end result is a plate containing spots of E. coli called colonies.

最后得到的是一块含有大肠杆菌斑点的平板。

Methods The cDNA coding sequencing of enterokinase light chain was amplified by RT-PCR from mouse dodecadactylon mucosa and cloned into pET32a expression plasmid. The recombinant enterokinase light chain was expressed in BL21(DE3) and purified with Ni-affinity chromatography.

采用RT-PCR从C57BL/6J小鼠的十二指肠肠系膜黏膜组织中钓取肠激酶轻链的cDNA，将其克隆入pET32a原核表达载体中，并在大肠杆菌BL21(DE3)中进行表达，然后以镍亲和层析法对表达产物进行纯化。

Today's confined cattle live in their own excrement, which is the carrier of the deadly E.

现在的牛都生活在自己的粪便中，而这正是致命大肠杆菌E。

The stable expression of exogenous gene in E.coli chromosome had no effect on the bacterial growth and propagation.

外源基因在大肠杆菌染色体上的稳定表达并不影响细菌的生长繁殖。

Is the chimeric protein of human epidermal growth factor and PE40 which is recombinant protein of the translocated subunit and killing subunit of Pseudomonas exotoxin A.

EGF-PE4 0是由人表皮生长因子基因与绿脓杆菌外毒素 A的跨膜亚单位和毒力亚单位( PE4 0 )基因融合，在大肠杆菌内表达的重组毒素。

In the range of D_50-D_1, it showed an exponential curve. The equation is: Y ...

剂量率和菌量的变化对大肠杆菌的存活率影响不大。

Palmarosa is steam distilled from the grass and its scent is sweet. It is antiseptic, antiviral, bactericide, and febrifuge.

玫瑰草可以在5分钟内杀死大肠杆菌，是很有效的肠胃炎或肠道感染的杀菌剂。

Coli HB101 expressed fimbriae as well as E. coli HB101 indicating the involvement of MrkF in fimbriation.

此外，基因重组大肠杆菌HB101与HB101皆能产生纤毛，显示MrkF可能参与纤毛组装过程。

Then the flic gene was cloned into the E.coli expression vector pGEX-KG, which was induced with IPTG and the fusion protein was analyzed by SDS-PAGE and Western-blot.

构建重组表达质粒pGEX-flic，转化大肠杆菌工程菌BL21，经IPTG诱导表达后，通过SDS-PAGE及Western-blo对表达蛋白进行鉴定。

The flic gene of App was obtained by PCR based on highly homology with Escherichia coli, Salmonella, Shigellosis. Then it was legated to pMD-18T vector. The recombinant plasmid was identified by enzyme and sequence analysis. The 528 bp fragment of flic gene was successfully cloned into pGEX-KG vector, then the recombinant plasmid was transformed into E.

以猪传染性胸膜肺炎放线杆菌血清Ⅰ型菌株基因组为模板，根据其鞭毛区与其它细菌高度同源性设计引物，利用PCR方法扩增鞭毛蛋白基因flic片段，将其亚克隆到pMD-18T载体中并进行PCR和酶切鉴定，再将其亚克隆与载体pGEx-kG分别双酶切和连接，构建重组表达载体pGEX-flic，并将其转化大肠杆菌工程菌BL21，进行原核表达。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。