大肠杆菌

The fragment was connected to pcDNA3.0 carrier which was cut by the same two enzymes to form the plasmid which was transformed into Escherichia coli DH5a.Appropriate clone was cultured to extract plasmid.The extract was analysed to confirm that it was constructed correctively.

将该片段连接到同样被KpnⅠ和XhoⅠ双酶切的pcDNA3.0载体中，转化大肠杆菌DH5α感受态细菌，挑取阳性克隆进行质粒提取，进行酶切鉴定，并获得阳性克隆。

There are a great number of bacteria parasitizing in the digestive system of normal people like Escherichia coli, proteus, Klebsiella pneumoniae and Cryptococcus.

正常人的消化系统有大量的细菌寄生，如大肠杆菌，变形杆菌，克雷氏菌及隐球菌等。

Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.

maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表达载体 pET-20b 和 pET-28a，构建重组质粒 pET-20b-bglA 和 pET-28a-bglA，然后转化不同大肠杆菌 E.coli 宿主，Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中获得高效表达，重组蛋白的表达量为 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA)，可溶性比例为 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA)，比酶活达 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA)，这些结果表明，E.coli BL21-CodonPlus(DE3)-RIL 宿主带有的 argU tRNA、ileY tRNA 和 leuW tRNA 基因，分别增强对稀有密码子 AGA/AGG、AUA 和 CUA 的识别，有助于提高该酶在 E.coli 中的表达；进一步优化诱导条件，重组 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下诱导培养，IPTG 浓度由 1000 μM 降至 25 μM，目的蛋白可溶性表达由 12.3%增至 32.8%，提高 1.7 倍，16 ℃下低温诱导实现目的蛋白 70.6%的可溶性表达，较 37 ℃下诱导培养提高 1.2 倍。

So it is concluded that Escherichia coli O157:H7 can enter into the viable but non cul...

实验证明了大肠杆菌 O157：H7在一定的条件下可进入活的非可培养状态。

The expression plasmid pGEX-5X-1/DFF45 was constructed and transformed into E.coli BL21, and the expression of GST-DFF45 protein was induced by IPTG. After purification, the fusion protein GST-DFF45 was used to immunize Oryctolagus cuniculus to obtain the antibody serum.

构建pGEX-5X-1/DFF45原核表达质粒，转化大肠杆菌BL21，用IPTG诱导融合蛋白表达，经纯化后免疫日本大耳白兔得到多克隆抗体并用CNBr-activated Sepharose？

High level expression of Cysticercus cellulosae antigen cC1 was obtained in E.coli .

猪囊尾蚴抗原cC1在大肠杆菌中获得高效表达。

The fragment encoding the polypeptide of wheat MLO protein C terminus located in cytoplast was cloned into the vector pET-30a and expressed in the strain BL21 of E coli.

本研究将编码小麦MLO蛋白羧基端约130个氨基酸多肽的基因片断克隆到表达载体pET-30a中，在大肠杆菌中表达了该多肽的融合蛋白。

Over the last 10 years, antisense transgenic plants have been used as tools to address this and have revealed some unexpected findings. In this article, chloroplast tpi gene from spinach and tandem genes of chloroplast tpi gene from spinach and cytoplast fba gene from rice were transferred into filamentous, heterocystour cyanobacterium Anabaena sp.

将菠菜叶绿体tpi和水稻胞质fba的串联基因连接在光诱导的高效启动子PpsbA下游，克隆入pDC-08中构建了既能在大肠杆菌又能在蓝藻中表达的穿梭表达载体pDC-AT，并通过三亲接合转移转入鱼腥藻7120。

To explore the possibility of the targeting expression of Escherichia coli cytosine deaminase gene by adenovirus-mediated transfer in human pancreatic carcinoma cells.

探讨腺病毒介导的大肠杆菌胞嘧啶脱氨酶基因在胰腺癌细胞中靶向性表达的可能性及其作用。

Subtilase cytotoxin is produced by E. coli bacteria that cause bloody diarrhoea and haemolytic uraemic syndrome in humans.

亚麻酶胞毒素由大肠杆菌产生，能造成严重腹泻和溶血性尿毒综合症。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。