大肠杆菌

CDNA fragment of cadherin-like protein gene in the fifth instar larva of oriental tobacco budworm was amplified using degeneracy primers and RT-PCR technique, and the target fragment was further sequenced after being cloned into pMD18-T vector plasmid.

本研究以烟夜蛾五龄幼虫中肠的总RNA为模板，根据已经克隆的其他昆虫的类钙粘蛋白基因序列设计引物，利用RT-PCR技术扩增出了烟夜蛾类钙粘蛋白基因的cDNA片段，将其连接到pMD18-T载体，转化大肠杆菌后筛选阳性克隆并进行序列测定，测序得到的1335bP的片断编码444个氨基酸残基。

Insect resistance to Bt toxin is related with the variation of cadherin-like protein gene.cDNA fragment of cadherin-like protein gene in the fifth instar larva of oriental tobacco budworm was amplified using degeneracy primers and RT-PCR technique,and the target fragment was further sequenced after being cloned into pMD18-T vector plasmid.

本研究以烟夜蛾五龄幼虫中肠的总RNA为模板，根据已经克隆的其他昆虫的类钙粘蛋白基因序列设计引物，利用RT-PCR技术扩增出了烟夜蛾类钙粘蛋白基因的cDNA片段，将其连接到pMD18-T载体，转化大肠杆菌后筛选阳性克隆并进行序列测定，测序得到的1 335 bp的片断编码444个氨基酸残基。

This test generates a high- yielding GAD strain by mutagenizing and screening the escherichia colis. It is on the basis of this test to study on the optimal conditions of translation in this strain.

本试验以普通大肠杆菌经过诱变筛选得到一株高产谷氨酸脱羧酶菌株以此为基础，研究该菌株最佳转化条件。

Result The results showed that 34 compounds of the volatile oil were identified, which accounted for 90.10% of all. The main chemical compounds were camphene (10.030%), beta-pinene (8.826%), limonene (7.935%), caryophyllene (12.457%). The antibacterial experiments of the volatile oil showed that the volatile oil had antibacterial activities to Staphylococcus aureus, but had no inhibition against Escherichia coil and Rhodotorula glutinis.

结果］经毛细管色谱分析分离，共确认出其中34种成分，所鉴定出化学成分的质量占总量的90.10%以上，应用色谱峰面积归一法分析各成分的质量分数，其主要化学成分是莰烯(10.030%)、-β-蒎烯(8.826%)、柠檬油(7.935%)、丁子香烯(12.457%)；并发现其化学成分对金黄色葡萄球菌有较强的抑制作用，对大肠杆菌、红酵母无明显作用。

A horse-drawn caisson carries the remains of Army Capt. Ronald G. Luce, Jr., who was with 20th Special Forces Group, walking behind is widow Kendahl Shoemaker, and daughter Carle E. Luce, during funeral services at Arlington Cemetery in Arlington, Va., Wednesday, Oct.

马车沉箱进行的陆军上尉卢斯罗纳德湾小，谁与20特种部队小组仍然走寡妇Kendahl背后是梅克，和女儿弗卡尔大肠杆菌卢斯，在阿灵顿在阿灵顿公墓殡葬服务弗吉尼亚州，星期三，09年10月21日。

Extension of these pathways with other carotenoid modifying enzymes led to the production of novel structures in E.

扩大这些通路与其他类胡萝卜素修饰酶，导致生产的新型结构在大肠杆菌。

What consequences would a mutation in the catabolite activator protein gene of E. coli have for the expression of a wild-type lac operon?

大肠杆菌的代谢激活蛋白基因突变会对野生型的乳糖操纵元的表达产生怎样的影响结果？

Other interesting aspects of the enzyme and its gene are that intracellular PAC activity is under several regulatory controls, such as temperature, oxygen level, catabolite repression and phenylacetic acid induction.

另外，青霉素G酰化酶的基因表达调控非常复杂，在大肠杆菌中，至少有三个不同的启动子可以起动它的转录；可以在转录、翻译、分泌和折叠加工等不同环节进行调控，它的表达调控受温度，溶氧和碳源等环境因素的影响。

To study and know well the rudimentary knowledge and the basic techniques of modern Microbiology are demanded for Graduate students. The experiments including the principle and operation of phase contrast microscope and fluorescence microscope; Microphotography , the technique of photographic enlargement;Applications of polymerase chain reaction ; Construction, transformation and detection of plasmid in E.coli ; Infectivity of virus and its molecular detection ; The technique of protoplast fusion in filamentary fungi ; Induction and purification of β-galactosidase from E.coli and assay the enzyme activity; Detection of GC content and DNA-DNA hybridization in bacteria ; Production , extraction and detection of poly--hyduoxy butyrate from bacteria.

以实验教学为主，要求研究生学习和掌握一些现代微生物学研究所需的基本技术，内容包括：相差显微镜、荧光显微镜的使用和显微摄影技术，PCR技术的应用，细菌质粒的构建、转化及检测技术，病毒的侵染性及其分子检测技术，丝状真菌原生质体融合技术，大肠杆菌β-半乳糖苷酶的分离及纯化及其动力学测定技术，细菌G+C mol%的测定及细菌DNA-DNA杂交技术和聚羟基丁酸产生菌的发酵、产物提取及检测技术等。

Results as bellows: AtSIRT1 was located in Mitochondrial as hSIRT4 of human, and maybe take part in respiration and electron transformation chain, AtSIRT2 was located in nucleolus as hSIRT6 of human, maybe play important role in extend lifespan;mutation in AtSIRT1 leaded to cotyledon of plant turn to yellow and caused short life span. Mutation in AtSIRT2 could make the color of leaf turn to purple and accumulate a lot of anthocyanin;Sirtinol, a inhibitor of SIRT which did not cause the same model of the mutation of AtSIRT1 and AtSIRT2 indicated that the mechanism of Sirtinol was different from other organism;the structure of AtSIRT1 and AtSIRT2 were similar to other known Sir2, which indicated that they maybe have the same function;AtSIRT2 was overexpressed and its activity was detected.

结果表明，1，拟南芥AtSIRT1与人的同源蛋白hSIRT4相同，定位于线粒体，可能参与呼吸作用和电子传递，SIRT2与人的同源蛋白hSIRT6相同，定位于细胞核，可能同它的功能类似，在延缓衰老及调节细胞寿命方面起作用。2，AtSIRT1突变，可引起幼苗和植株的子叶变黄和早衰；AtSIRT2突变，可引起叶片发紫，沉积大量花青素。3，SIRT蛋白的抑制剂Sirtinol不能表型模写AtSIRT1和AtSIRT2突变体，说明Sirtinol在拟南芥中的作用机制不同于其他生物。4，AtSIRT1和AtSIRT2蛋白质结构预测表明与已知的Sir2蛋白相似，揭示其功能的相似性。5，在大肠杆菌中过量表达了其中一个基因(AtSIRT2)，可体外检测其酶学活性，进一步证明其功能。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。