大肠杆菌

We optimized reaction system for labelling-antibody in which the optimal amount of the purified anti-Stx2B IgG conjugated with colloidal gold beads was 60μg/mL, the purified antibody for E.coli O157 was 57μg/mL, the optimal pH was 8.2, the size of colloidal gold partical was 20nm, the optimization of stabilizing agent was BSA, the optimization buffer was boracic acid buffer with pH 8.2, the optimization of preserving fluid and eluant was boracic acid buffer with pH 8.2 including 5mM NaCl-1%BSA, confining liquid for NC membrane was 0.01% PBST with 3%BSA, the amount of polyclonal antibody against E.coli O157 and Stx2 conjugated with colloidal gold beads for conjugate pad was 3μg respectively, the amount of anti-Stx monoclonal antibody for test line was 0.1μg and 1μg for E.coli O157, the amount of goat anti rabbit IgG for control line of both GICA were 1μg.

测定了胶体金颗粒的最优化反应体系：胶体金颗粒的大小为20nm；抗体与胶体金溶液结合的最佳pH约为8.2；最佳蛋白结合量分别为抗大肠杆菌O157 IgG为57μg/mL，抗重组Stx2B IgG为60μg/mL；最佳稳定剂为BSA；最佳缓冲液为pH8.2硼酸溶液；最佳金标保存液和洗涤液为5mM NaCl-1%BSA的pH 8.2的硼酸缓冲液；NC膜的封闭液为3%BSA的0.01mol/L PBST；Stx2试纸条和大肠杆菌O157试纸条的质控线上的羊抗兔IgG的多克隆抗体最佳点样量均为1μg，其检测线的抗Stx2的单克隆抗体的点样量为0.1μg、抗大肠杆菌O157的单克隆抗体的点样量为1μg，结合垫上的金标抗大肠杆菌O157和重组Stx2B多克隆抗体点样量均为3μg。

Experimental results indicated that the silver nanoparticles of 20 μg/mL could inhibit completely the growth of 10^6 cfu/mL cells in liquid LB medium. The growth curves showed that silver nanoparticles prolonged the lag phase of E. coli, and the higher of the concentration of silver nanoparticles, the longer of the lag phase of E.

纳米银对大肠杆菌的抑制生长曲线的结果表明，20μg/mL的纳米银能够完全抑制10^6 cfu/mL的大肠杆菌细胞生长，纳米银使大肠杆菌的延滞期加长，并且纳米银浓度越高，延滞期越长。

Three kinds of BCRC No.51534, 10322 and 10675 would be selected and acted as an experimental sample of Escherichia coli. Results shows that Escherichia coli of No.51534 will appear better performance because the maximum of open circuit voltage, closed current and power density are 1.01V, 22mA and 1342mW/m2, respectively. Concerning the effect of culture time with respect to different phase type on the electricity performance of MFCs, the time points on the intersection between lag phase and logarithmic phase, the middle of point of stationary phase for growth curve of Escherichia coli would appear a good performance of MFCs. In addition, the BCRC No. 51534 Escherichia coli possessing a better performance of MFCs than others would be suggested and applied to further studying. Comparison with the performance of MFCs with respect to electron mediator under different mole number, result shows that electron mediator of methylene blue with 4.63mM would appear a better electricity performance of MFCs than others. Concerning the different material of proton exchange membrane with PTFE-Nafion, Nafion 211, 212 and 117 with respect to the performance of MFCs, result shows that the Nafion 117 applied in MFCs will have a better performance of MFCs than other cases. Finally, the effect of molar concentration on the performance of MFCs would be expected at the studied cases of 0.4M, 0.2M, 0.1M and 0.05M respectively for cathode oxidant, result shows that a good performance of MFCs will happen at the condition of 0.2M. Those observations will be useful to improvement of MFCs in the further study.

於上述电池系统条件下，进行大肠杆菌生长曲线、电子传递介质、质子交换膜、电极与阴极氧化剂对电池电性效能分析；选择编号10322、10675与51534之大肠杆菌为实验菌株，依定量培养之生长曲线取出代表不同时生长特性时期的培养时间，利用亚甲基蓝作为电子传递介质进行实验分析从所测得的电量进行分析，以编号51534之大肠杆菌的微生物燃料电池有最大的开路电压为1.01V及最大闭路电流为22mA；当极化曲线中电压为0.47V、电流为11.4 mA时有最大的功率密度为1342 mW/m2；加以负载有平均工作功率密度294 mW/m2；从生长曲线与电性效能来分析，得知生长曲线的迟滞期与对数期的转变点与静止期的中间点有最佳电性效能表现；对於加入不同莫耳数之电子传递介质methylene blue、neutral red与thionine之电池效能表现，则以加入4.63mM methylene blue电子传递介质的电池有较佳平均功率密度230 mW/m2；另对於质子交换膜PTFE-Nafion、Nafion 211、Nafion 212与Nafion 117之电池效能表现，以Nafion 117质子交换膜的电池有较佳平均功率密度340 mW/m2；对於分析加入不同莫耳数浓度0.4M、0.2M、0.1M与0.05M的阴极氧化剂之电池效能，则以0.2M的阴极氧化剂的电池可得到较佳平均功率密度429 mW/m2。

The result indicated: antibacterial concentration of inner thallus increased progressively with time prolongation when added CCCP with antibacteria, proved that multi-resistance of E.coli in livestock and avian is mediated really by active efflux system; The multi-resistant level have positive correlation with AcrAB transcription and expression level; Quantitive RT-PCR,Western-blotting and fluorescence quantitive PCR can use to detect AcrAB.these results provide theory foundation to detect and study multi-resistance of E.coli in veterinary clinic and have a important scientific significance to develop neotype antibacterials.

结果表明，多重耐药株在有CCCP 存在时，菌体内抗菌药物的浓度随时间的延长而递增，证明畜禽大肠杆菌多重耐药性确实由主动外排系统介导；其多重耐药水平与AcrAB的转录、表达水平呈现正相关；定量RT-PCR、Western-blotting、荧光定量PCR可用于AcrAB水平的检测。这些结果对检测临床大肠杆菌的多重耐药水平、对研究兽医临床大肠杆菌多重耐药机制提供理论依据，对开发新型抗菌药物有重要的科学意义。

Chickens colibacillosis is a focus or systemic infectious disease, which is caused by E.coii ,with complicated antigens, multiple serotype and various clinical symptoms such as salpingitis, synovitis, and so on. Colibacillosis often happens together with various diseases and has been spread everywhere in Haian county in different extent..

鸡大肠杆菌病乃是由大肠埃希氏菌所引起的局部或全身性感染的疾病，其抗原性复杂，血清型多样，临床症状和病理变化多变，包括大肠杆菌性败血症、大肠杆菌性肉芽肿、气囊炎、肿头综合症、腹膜炎、输卵管炎、滑膜炎、全眼球炎及脐炎、卵黄囊炎等。

The adhesion test showed that small intestinal epithelium cells of 30-35-day-old postweaning piglets with both genotype M307~ and M307~ could adhere to the standard E.coli strain express F18ab fimbriae, the recombinant E.coli express F18ac rE.coli1534 and the recombinant pnirBMisL-fedF E.coli displaying FedF subunit on the surface of E.coli, but small intestinal epithelium cells with genotype M307~ and 3-day-old piglet could not adhere to the three kinds of bacteria descried above, the latter with good adherence capability of 987P fimbrial E.coli.

9仔猪小肠上皮细胞的黏附试验结果显示，30～35日龄M307~基因型和M307~基因型断奶仔猪的小肠上皮细胞均能与表达F18ab菌毛标准菌株107／86、诱导表达F18ac菌毛的重组大肠杆菌rE.coli1534及诱导菌体表面展示表达F18黏附亚单位FedF的重组大肠杆菌pnirBMisL-fedF发生黏附作用，而同日龄M307~基因型断奶仔猪的小肠上皮细胞均不能与上述三株大肠杆菌发生黏附作用。3日龄易感仔猪小肠上皮细胞也不能黏附上述三株大肠杆菌，但可以很好地黏附表达于987P菌毛的大肠杆菌。

Under hypotonic condition, the surrival rates of pI〓-expessing cells were significantly higher than that of control cells.

低渗条件下进行大肠杆菌菌落存活率的检测，结果显示有pI〓表达的大肠杆菌菌落存活率明显高于无pI〓表达的对照组大肠杆菌的菌落存活率。

The power-time curves of E.coli reacting with Co-SG at different temperature has been determined by microcalorimtric method The multiplication rate constant k, generation time G,bacterial growth inhibition ratio I,total thermogenetic quantity Q, the heat quantity of a single bacterium Q_(0) and the heat quantity of a single bacterium per minute have also been calculated according to the power-time curves.

用微量热法测定了大肠杆菌及其在Co -SG配合物作用下的热谱曲线，计算了速率常数k ，传代时间G、抑制I、总产热量Q、单个细菌的产热量Q0 和每分钟单个细菌的产热量 Q0 ，建立了部分代谢参数之间的关系，探讨了大肠杆菌在不同温度和Co -SG作用下的代谢抑制，发现可用t、tr、tg和 Q0 定量表征在不同温度下大肠杆菌的生长代谢和药物的抗菌活性。

Through the mediation of Nodule Agrobacterium, the insecticidal protein gene from pseudomonas pseudoalcalgenes has been transformed into the receptor materials to develop a new transgenic forage grass with the character of grasshopper resistance.

类产碱假单胞菌杀虫蛋白基因在大肠杆菌中的表达由于大肠杆菌的启动子不能识别类产碱假单胞菌杀虫蛋白基因，因此ppip基因不能直接在大肠杆菌细胞中表达。

The most pathogenic isolate, VIB 645, contained two closely related hemolysin genes, which were cloned and sequenced. In this study, vhhA was cloned into an expression plasmid pET-24d.

本研究将哈维氏弧菌的溶血素基因vhhA克隆到大肠杆菌的表达载体pET-24d，VHH溶血素作为一种带六个组氨酸的融合蛋白在大肠杆菌表达菌株BL21(DE3)中得到了过量表达，重组大肠杆菌及其培养上清液在鱼血平板上有很强的溶血活性。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。