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Methods Unit responses were recorded extracellularly from thalamic ventroposterolateral nucleus in anesthetized rats.

采用在体细胞外单位放电记录技术,记录麻醉状态下大鼠丘脑腹后外侧核触觉神经元的放电活动。

The unit discharge of auditory neuron in the cortical AⅠ area was recorded with the extracellularly recording method. The character of ON-OFF responses induced by short pure tone and the influence of stimulating lateral amygdaloid nucleus on these responses and the tuning curves of the recorded neurons were examined.

在30只氨基甲酸乙酯麻醉的SD大鼠上记录神经元单位放电,观察短纯音诱发的皮层AⅠ区神经元ON-OFF反应的特性及电刺激杏仁外侧核(lateral amygdaloid nucleus, LA)对ON-OFF反应以及调谐曲线的影响。

The results show as follows:(1) immunoreactive neurons of NPY in pigeon cerebellum tire mainly located in the Purkinje cell layer of the cerebellar cortex, especially in the top of Purkinje cell in cerebellar folia:(2) a few neuropeptide Y immunoreactive neurons are observed in the central nuclei of cerebellar white matter .which is different from silky fowl, chick and rat;(3) none of neuropeptide Y immunoreactive neurons are found both in the molecular and granular layer.

结果显示:(1)小脑内神经肽Y(neuropeptide Y,NPY)阳性神经元主要存在于小脑皮质的蒲肯野氏细胞层,且以小叶顶端的蒲肯野氏细胞阳性明显;(2)小脑白质中央核可见到少量阳性神经元,不同于乌鸡、肉鸡及大鼠;(3)分子层、颗粒层未见阳性反应细胞。

The effects of electrical and chemical stimulation at nucleus rape dorsalis and nucleus raphe magnus on genioglossus and phrenic muscle activities and amplitude augmenting response to apnea were observed.

本实验在64只氨基甲酸乙酯麻醉、断双侧迷走神经、自主呼吸的健康家兔上进行,观察电、化学刺激中缝背核、中缝大核对颏舌肌和膈肌肌电、以及窒息增幅反应的作用。

MSCs were cultured in a conditional medium in subculture including: dexamethasone, vitamin C, and β-glycerophosphate. At the same time, we constructed the eukaryotic expression vector containing VEGF165gene. VEGF165 gene was transfected into MSCs by means of LipofectAMINE?2000. The stably gene expressive cells were screened with G-418 for 14 days.

在脂质体介导下将构建成功的真核表达载体pcDNA3.1- VEGF165转染到诱导培养的MSCs,G-418筛选阳性细胞克隆,将阳性克隆细胞和未转染的MSCs共同接种于β-磷酸三钙上,体外培养7天后,植入原大鼠肌肉内。

Morphology of sexual reproduction abortion in'High Noon', a distant hybridization cultivar of tree peonies(P.delavayi var. lutea×P. suffruticasa), was systematically investigated with light microscopy and cross. The results indicated that:(1)The stamens matured earlier than the gynoecia and pollens dispersed at the early stage of anthesis but the rate of normal 2-celled pollens were merely 2.41%.

采用光镜和杂交等方法对牡丹远缘杂交一代品种'正午'有性生殖败育进行系统形态学观察发现:(1)雄蕊较雌蕊先成熟,初花期即散粉,正常2-细胞花粉仅占2·41%,且花粉在多种培养液中、在自身和其它两种柱头上均不萌发;柱头在谢花期大量分泌粘液,进入最佳授粉状态;(2)同一胚珠中多个大孢子母细胞减数分裂高度不同步,胚囊发育与受精延迟,部分胚乳游离核在细胞化时期分裂异常;(3)自花及品种内异花授粉不结实,天然授粉结实率为0·053‰~0·130‰。

Ingredients: Alfalfa Sprout 80g, sweet potato leaves 75g, gynura 75g, pineapple 150g, 1 apple, 1 tomato, 30 pine nuts, black sesames 10g, mix grain powder 8g each, soup (Astragalus membranaceus Bunge, red jujube, Matrimony vine) 200c.c.

材料:苜蓿芽80g 、地瓜叶75g 、红凤菜75g 、菠萝150g 、苹果1个、大西红柿1个、松子30粒、黑芝麻粉10g 、三宝粉各8g 、黄蓍红枣枸杞汤200cc 。苹果洗净去皮、核、切块;西红柿洗净去蒂切丁;菠萝去皮切块;苜蓿芽、地瓜叶、红凤菜洗净、切碎。

HPLC was used to detect the content of monoamine transmitter in habenula of the young and adult female rats with different levels of sex steroid.

用高效液相-荧光检测法分别测定了成年及幼年雌性大鼠缰核内单胺类递质的含量。

Methods:Arterial blood pressure,heart rate and the unit dischargings of habenular neurons in rat were recorded simultaneously.

记录大鼠的股动脉血压和心率的同时,用玻璃微电极记录缰核神经元自发单位放电。

Results:Arterial blood pressure was apparently decreased by 10 mg/kg losartan intraperitoneally,but heart rate did not change;63.64%(21/33) unit dischargings of habenular neurons was increased in the rate of discharges.

结果:腹腔注射10 mg/kg芦沙坦可显著地降低大鼠动脉血压,但对心率无明显的影响;芦沙坦可使缰核64%(21/33)自发单位放电的放电频率增加。

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