大核

Objective To detect the effects of ethology, the neuron and glia of dorsal commissural nucleus in rats with irritable bowel syndrome induced by distented the colon, and provide theory evidence for the pathogeny of IBS.

目的 采用行为学和免疫荧光化学的方法，观察直肠扩张刺激对IBS大鼠痛行为学以及大鼠骶髓后连合核(dorsal commissural nucleus， DCN)中神经元和胶质细胞的影响，为探讨IBS发生机制提供理论依据。

The remaining 9 rats in control group, 16 rats in treated grouop and 15 rats in model group were decollated and obtained brains to detect the relative mRNA expression of nuclear receptor-related factor 1 (Nurr1) and muscarinic acetylcholine receptor 5(M5) byusing RT-PCR, which was the consequence of those comparing with reference geneβ-actin mRNA expression.

将对照组、治疗组、模型组其余9、16、15只大鼠，断头取脑，采用RT-PCR方法检测大鼠脑组织孤儿核受体及乙酰胆碱毒蕈碱型受体5(M5)mRNA相对于内参基因β-actin mRNA的相对表达值。

The effects of intracerebralventricular injection of nitric oxide donor and nitric oxide synthase inhibitor on the spontaneous discharge of magnocellular neurons of paraventricular nucleus of hypothalamus were investigated in urethane anaesthetized adult SD rats by using microelectrode extracellularly recording method.

在乌拉坦麻醉的成年SD大鼠上，用玻璃微电极细胞外记录的方法，观察了脑室内注射一氧化氮供体及一氧化氮合酶抑制剂对室旁核大细胞自发电活动的作用。

The Taohezhenwu Decoction has good curative effect in treating and preventing CRF of rats induced with adenine by improving the renal function, electrolyte disturbances, acid-base equilibrium, hemophthisis and the function of SOD, reducing the harm of NO, TNF-α, and lessening or delaying the deterioration of nephron.

桃核真武汤能改善腺嘌呤大鼠慢性肾衰模型的肾功能、调节电解质酸碱平衡紊乱、纠正贫血、减少尿蛋白的丢失、减少NO与TNF-α损伤、提高SOD的保护作用、一定程度上防止肾单位的恶化，对SD大鼠腺膘呤所致之CRF的防治有肯定的疗效。

Internal transcribed sequences of ribosomal DNA of 36 species wereanalyzed to reconstruct the phologeny of Heracleum and its related genera using themaximum parsimony, neighboring-joint and maximum likelihood methods inPAUP4.0b10, respectively.

选取独活属7种植物测取核rDNA中的ITS基因序列，结合GenBank已有的本属5种和邻近属24种植物的ITS序列，用PAUP4.0b10软件中的最大简约法、邻接法和最大似然法分别进行分析，从而对独活属及其近属进行了系统发育重建。

Results:17 rats were confirmed successful transplantation by MRI,the capacity of the tumor was 88.40±7.62mm3 on 8th day, 986.80±114.46mm3 on 16th day,the volume growth was 8-12 folds;on 8th day following transplantation,the tumor tissue was gray in color with fish homogen state without interior necrosis, the tumor cells were nest-distributed by HE staining,large dense staining of nucleus with manifest heteromorphism,tumor microvessel count increased by immunohistochemistry with buffy yellow staining, VEGF high expressed in the tumor cells with buffy yellow granule shape.

结果：17只大鼠经MRI检查证实均种植成功，肿瘤体积第8天为88.40±7.62mm3，第16天为986.80±114.46mm3，体积增长8-12倍之间；种植第8天，肿瘤组织呈灰白色、鱼肉均质状，内部未见坏死，HE染色肿瘤细胞呈巢状分布，胞核大浓染，异型性明显；免疫组织化学示肿瘤微血管数目较多，呈棕黄色染色，肿瘤细胞VEGF高表达，呈棕黄色细颗粒状。

The changes were observed by microinjecting Larginine, Darginine, sodium nitroprusside and hemoglobin into rat caudate nucleus in 30 minuts. Results:The significant hyperalgesic effects were observed by microinjecting LArg and SNP into caudate nucleus of rats.

以钾离子透入引起大鼠甩尾的电流强度作为痛反应指标，尾核内微量注射L精氨酸、D精氨酸、硝普钠和血红蛋白等，观察0～30min内大鼠痛阈的变化。

Methods: Thirty adult male Wistar rats were randomly divided into three groups: the operation group, the transplantation of MSCs group and the injection of saline group. Functional outcome measurements were performed based on the modified neurological severity score at day 1, 7, 14, 21 and 28 after MACO. The survival, migration, expression of Nestin, glial fibriliary acidic protein and neuronspecific enolase of 5bromo2deoxyuridinelabeled MSCs were detected by immunohistochemical double staining.

线栓法建立左侧大鼠MCAO模型，随机分为3组：手术组、MSCs 移植组、生理盐水组。5溴2脱氧尿核苷标记的MSCs移植后，采用改良神经功能损伤评分系统评价大鼠神经功能恢复情况；应用免疫组织化学双染技术检测MSCs的存活、迁移及其巢蛋白、胶质纤维酸性蛋白和神经元特异性烯醇化酶的表达。

Finally, in multinuclear macrocyclic polyamine derivatives, we found that cyclen-based oligomers form polymer complex with DNA, thus protect DNA from enzymatic degradation efficiently.

在多核大环多胺方面，我们合成了基于大环多胺的寡聚物，研究发现该类物质可与DNA形成复合物，从而有效地保护DNA免于酶解。

The macrocyclic polyamines are a kind of excellent ligands and have a substantially high binding affinity with transition metal ions. Macrocyclic polyamine metal complexes as enzyme models are widely used in chemical biology, medicine and gene therapy. 1, 4, 7, 10-tetrazacyclododecane is a representative macrocyclic polyamines compound and shows high binding affinity with transition metal ions, such as Zn, Cu and Co. Their metal complexes especially multinuclear complexes were widely used in DNA, RNA recognition.

大环多胺化合物是一类性质独特的优秀配体，具有良好的金属配位能力，其金属配合物作为人工酶模型广泛应用于核酸结构及功能研究、基因表达与调控、抗癌药物的研制等生物、化学和医学领域。1，4，7，10-四氮杂十二烷是典型的大环多胺化合物，对过渡金属离子Zn， Cu and Co有良好的配位能力，这些金属配合物特别是多核金属配合物被广泛应用于DNA识别中。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。