大核

The double labelling technique by combination of horseradish peroxidaseretrograde tracing with immunohistochemical staining for γ-amino-butyric acidwas used to observe the distribution of GABA-positive neurons in the abducens and vestibular nucleus,and its projection to oculomotor nucleus of rats.

用辣根过氧化物酶(horseradish peroxidase，HRP)逆行标记结合γ-氨基丁酸(γ-amino-butyric acid，GABA)的免疫组织化学双标技术观察大鼠的展神经核和前庭神经核内GABA阳性神经元的分布，以及其向动眼神经核的投射。

Our results have shown that the analgesic effect of morphine administered to nucleus accumbens was blocked by intra-habenula injection of naloxone or ME antiserum, whereas the analgesic effect of morphine injected into habenula was attenuated by intra-PAG administration of naloxone or muscimol, and enhanced by bicuculline methochoride (BMC, an antagonist of GABA receptor).

本工作实验结果表明，在伏核内注射吗啡产生的镇痛作用可被缰核内注射NX或ME抗体所大部阻断，而缰核内注射吗啡产生的镇痛作用又可被PAG内注射NX或γ-氨基丁酸受体激动剂异鹅羔胺所削弱，被GABA受体阻断剂氯甲基荷包牡丹碱所增强。

The new method has better resolution and can be applied to larger systems than homonuclear NMR methods. Most proteins playing an important role in cells have to be studied with heteronuclear labeled samples.

异核方法的优势在于它的分辨率更好，可以研究相对较大的，同核方法研究不了的分子；对大部分在细胞中发现的意义重大的蛋白的NMR研究都必须采用异核方法。

Results Apoptotic cells were found among normal dentate hilus cells and CA1 cells. Chromatin condensation and clumping could be seen in the apoptotic nuclei. The shrinkage of the nucleus was shown by foldings and whorls of the nuclear membrane. At late stage of apoptosis, the nuclear membranes could be broken.

结果 电镜下，实验组大鼠海马齿状回门区和CA1区内可见散在的凋亡神经元，凋亡神经元主要表现为核周染色体的聚集和凝结成块，其核膜表现为皱缩和扭曲，在晚期凋亡的神经元有时可见核膜破裂。

It was found in this investigation that boron exerted considerable influenceon the nucleation and growth of pearlite. In a hypoeutectoid steel, boron delayedthe starting time of pearlite formation at the maximum pearlite formation temp-erature. Boron did not effect the relation of nucleation rate with time butonly increased the incubation period for pearlite nucleation.

从实验结果证明，硼对珠光体的成核及成长是有影响的；在亚共析钢中硼促使珠光体的开始形成时间推迟，增长了珠光体成核的孕育期，但对成核率与时间的关系，在近于A_1温度(700℃)时，略为加速，而在珠光体转变速度最大的温度(650℃)时却没有影响。

In this paper, a kernel quantification theory IV is proposed through organical combining kernel function theory and quantification theory IV. The algorithm framework for the new model with large scale-sampling data is established on the basis of Lanczos algorithm which is an iterative method for finding the eigenpairs of a square matrix.

把核函数理论与数量化理论IV模型有机结合，提出了核数量化理论IV模型，并以高阶对称矩阵端部特征对求解的Lanczos算法为基础设计了大样本核数量化理论IV模型的算法框架。

Based on the results, it has found that six distinct layers can be identified from lateral to medial in the olfactory bulbs, and the adults have more cells than the subadults. Ventricles of the adults take up larger vacuity, have richer choroids plexus, and primordial pallium and primordial hippocampus of them are more developed than the subadults, in which the primordial piriform is well-developed. Ventral to the primordial hippocampus, there is a septal area which can not be divided into medial and lateral parts in both adults and subadults, nor there is lateral limiting sulcus to separate the primordial piriform and the corpus striatum, but only in the subadults, a medial limiting sulcus can be clearly identified to separate the primordial hippocampus and the septal area. Besides, the corpus striatum of Onychodactylus fischeri is paleostriatum. The cell group that located at two sides of the third ventricle is amygdale, and shape and size of neurons within the telencephalon are poorly differentiation.

结果表明：爪鲵嗅球内细胞从外到内大致可分为6层结构，亚成体的细胞数量少于成体；成体爪鲵的侧脑室所占空间较大，脉络丛更为丰富，原始大脑皮层和原始海马都比亚成体发达，后者大脑内原始梨状区较为发达；隔区位于原始海马的下方，成体和亚成体都没有外侧隔核和内侧隔核之分，也没有外侧界沟作为原始梨状区和纹状体的分界，而在亚成体的侧脑室壁有内侧界沟区分原始海马和隔区，成体则没有；此外，爪鲵的纹状体属于古纹状体，杏仁核是位于第三脑室两侧的两个细胞团，端脑内细胞形态和大小分化较为单一。

Increased expression of PENK mRNA was more marked in the ipsilateral dorsal horn of the spinal cord, especially in lamina Ⅲ-Ⅳ and the contralateral medulla, especially in the gigantocellular reticular nucleus, lateral paragigantocellar reticular nucleus and lateral reticular nucleus.

本实验用原位杂交技术对电针刺激后前脑啡肽mRNA在脊髓和延髓的表达进行了观察，发现给予大鼠单侧后肢电针刺激引起脊髓和延髓中PENK mRNA阳性神经元数显著增加，尤其是同侧脊髓背角各层PENK mRNA的表达，和对侧延髓网状结构如网状巨细胞核α部、网状巨细胞外侧旁核及外侧网状核中的PENK mRNA表达增加，这与脊髓上传的信号由同侧脊髓交叉到对侧脑干的走行方式是一致的。

Periplaneta fuliginosa is the major species of cockroach distributed in urban and rural area of our country.

黑胸大蠊是在我国城乡室内分布的主要蟑螂种类，黑胸大蠊浓核病毒(Periplaneta fuliginosa Densovirus， PfDNV)是国内外第一个分类鉴定的蟑螂浓核病毒。

The giant panda-rabbit interspecies reconstructed embryos were constructed by injection of giant panda somatic cells into the perivitelline space of the enucleated oocytes and fused by electrical stimulation, and the reconstructed embryos can develop to hatched blastocyst stage in vitro. We demonstrated that the nuclear genetic materials in reconstructed blastocyst cells were the same as that of giant panda somatic cells by chromosome count and the existence of characteristic chromosome, which was confirmed by microsatellite DNA analysis later In order to determine the fate of mtDNA of giant panda-rabbit reconstructed embryos, we analyzed the cyt b sequence of embryos at 1 -cell stage, 2-cell stage, 4-cell stage, 8 cell stage, morula stage, blastocyst stage, expanded blastocyst stage and hatched blastocyst stage.

异种克隆大熊猫重构胚是通过将大熊猫成纤维细胞移入日本大耳白兔去核卵母细胞透明带下后经电融合构建的，可在体外发育至孵化囊胚阶段，我们应用一种简便、快速的异种重构胚的鉴定方法，即通过染色体数目和特征染色体的存在初步鉴定出大熊猫-兔异种重构胚的核遗传物质来源于大熊猫供核体细胞（后经昆明动物研究所通过微卫星DNA分析验证了该结果的可靠性）。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。