大改变

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Methods1. Compared with adult female rats, aged female rats without oestrum were chosen by using RIA technology and sexual hormone was changed remarkably. Theses aged rats were divided into three groups（GNK group, HRT group and control group）. Treated them respectively with GNK, nilestriol and distilled water 3 months.

通过用阴道脱落细胞涂片检测发情周期的改变，放免法测定血清性激素的水平，与正常青年雌性大鼠比较，选择阴道脱落细胞无发情期改变，血清性激素水平有增龄性改变并且差异有显著性的老年雌性大鼠作为研究对象，分别用中药复方更年康和尼尔雌醇灌胃3个月，观察中药复方更年康对老年雌鼠的治疗作用。2。

Results: No significant difference were found befween A and B or C and D group before administration of TMP. In compare with the control group, TMP might increse HR and CO but decrease BP. The most marded changes of BP and CO appeared immediately agter the administration of the drug and refurned to the same leoel as premedication 5-7min later. The ± LVdp/dtmax and Time- tenson- index decreased firstly and increased later, reached the peak at 5min. In AMI group no obvious changes was found in HR. but BP decreased and CO increased.

注药前各监测指标在A、B两组及C、D两组间均无显著性差异，与A组相比，TMP可使健康大鼠出现明显的HR增快，BP下降，以及CO的增加，BP和CO改变的最大值均出现在给药即刻，5～7分钟内逐渐恢复至给药前水平，±LVdp/dtmax和时间一张力指数为先降后升，5分钟时达最高峰；在AMI大鼠组，TMP对HR无明显改变，但出现BP下降和CO增加，对±LV dp/dt max和TTI只有短暂的抑制，l～3分钟后恢复至用药前状态，无增强作用。

Objective To examine the value of Edaravone in the treatment of vascular dementia through ethologic and pathologic changes induced by chronic cerebral ischemiaMethods Wistar rats were randomly divided into sham operated group,model group,edaravone group and duxil groupThe chronic cerebral hypoperfusion models were made by permanent bilateral occlusion of both common carotid arteriesThe memory ability and ultramicrostructure of brain tissue were observedResults The escape latencies in model group were more significantly prolonged than that in the other groups (P＜005)But there was no significant difference between edaravone and duxil groupAnd pathologic changes observed by electron microscope in nerve cell of edaravone group and duxil group and model group were fairly light than that in sham operated groupEdaravone group had increased mitochondrium and frontal membranous synaptic vesicle and more productive metabolic activityConclusions Edaravone can lessen injures of hippocampal neurons loss to improve the spatial learning and memory abilitiesAnd it is proved further that edaravone can treat VD

目的 观察依达拉奉对慢性缺血致血管性痴呆大鼠行为学及形态学的影响，探讨依达拉奉在治疗VD中的应用价值。方法实验分假手术组、模型组及都可喜对照组及依达拉奉治疗组，应用双侧颈总动脉结扎方法制备慢性脑缺血大鼠痴呆模型，观察各组大鼠的记忆功能及脑组织超微结构改变。结果缺血组水迷宫表现同对照组、都可喜组及依达拉奉组相比有显著差异(P＜005)，依达拉奉组与都可喜组相比未见显著差异。同时电镜观察结果也发现依达拉奉组及都可喜组与模型组及假手术组相比神经细胞病理改变较轻，且依达拉奉组线粒体及前膜突触小泡增多，代谢活动较都可喜组旺盛。结论依达拉奉可能通过减轻海马神经元的损伤来改善慢性脑缺血大鼠空间学习记忆障碍，证明依达拉奉对慢性缺血致VD大鼠具有治疗作用。

Change is unpredictable, change is risky, and change is painful, and most immortally, is most of time, as I mentioned during the interview, we do not know what to change, when to change, how to change to minimize loss and maximize gain, and that requires leadership.

变化是难以预料的，变化是冒险的，而变化是痛苦的，而最千古，是大部分时间，正如我在采访中，我们不知道会有什么改变，何时改变，如何改变，以最大限度地减少损失，最大限度地获取，它需要领导。

Objective To establish rat model with polycystic ovary with two different methods:testosterone plus human chorionic gonadotropin and progestogen plus HCG,compare the rat models induced by testosterone plus HCG with the patients with polycystic ovary syndrome in the aspects of ovarial morphous and gonadal hormone,and investigate whether testosterone with HCG is the effective method to establish rat model with polycystic ovary syndrome,than compare two methods(T+HCG and P+HCG)finally to draw a conclusion if there are any difference or similary between the two methods and which one is more ideal and convenient.Methods 60 SD female rats of 23 days were divided into three groups in random.

目的 本实验用睾酮联合绒毛膜促性腺激素及炔诺孕酮联合HCG两种方法分别建立多囊卵巢大鼠模型，将模型鼠的卵巢形态及血清激素方面改变与人类多囊卵巢综合征的病理改变加以比较，探讨睾酮联合HCG是否是建立多囊卵巢大鼠模型的有效方法，并比较两种造模法是否存在异同点，判断哪一种造模方法更为理想及简便有效。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

And destruction usually occurs on angle.(2)Around the keyhole, the stress centralizes, column" edge opt to inflects in local.(3)The change of friction coefficients or bolt pretension forces can"t affect the performance of top-and-seat angel connections so much.(4)The parameters of angel influence joints performance greatly.

破坏一般是由于顶角钢上应力过大，形成塑性铰而导致的；(2)柱翼缘在与顶角钢相连的螺栓孔周围，存在应力集中，容易导致局部屈曲；(3)材料抗滑移系数的改变和螺栓预拉力的大小对于节点受力性能的影响并不明显；(4)角钢参数的改变对节点的各项性能影响显著，增加角钢厚度，节点的强度、刚度和延性都能得到明显的提高；增加角钢长度，节点的强度和刚度也有较大的提高，延性则略有下降；(5)柱轴压比过大时，节点的承载力、刚度以及变形能力明显下降；(6)螺栓中心线到角钢肢背距离增大时，节点的强度和刚度有所下降，但延性略有增加。

Study of peripheral nerve lesions and the effects of the recipe of supplementing Qi and activating blood circulation on it in streptozotocin-induced diabetic rats: It was showed that the weight, MNCV and SNCV were significantly reduced, H reflex were significantly prolongated, the level of blood sugar were significantly increased in diabetic rats compared to normal rats at 1 week, 3 week and 5 week after STZ injection (p.05), some changes of the sciatic nerve structure were occurred after 5 weeks of the diabetic progress.

STZ大鼠周围神经功能变化及益气活血方对其影响的实验研究：STZ诱发的糖尿病大鼠，成模1W后体重减轻、血糖升高、SNCV、MNCV减慢、SNCVamp减小、SNCVlat及H反射延迟，以上各项指标与对照组相比差异显著（P均。05）：成模5W后上述改变更为明显。成模5W后大鼠坐骨神经纤维出现髓鞘变性、融合、部分神经纤维变性等形态改变。

Results Group C had the symptoms such as reduced activity, acceded, indulge in lying and weight loss after 3 weeks of immune injection, 14 out of 16 SD rats in Group B had the same symptoms as Group C after 4～5 weeks of immune injection, the serum enzymes in model groups increased significantly compared with those of the control group, model group C was much higher than model group B; the duration shorted, amplitude decreased, multiphase wave increased in electromyogram of model groups; MRI examination revealed samples from model group B and C had one positive case each, which presented T1MI isodensity or hypodensity signal, T2MI and STIR serial hyperdensity signal, revealing muscle inflammation; all rats'skeletal muscle from model group C and 11 out of model group B had pathological changes, which exhibited striated muscle focal fiber degeneration, necrotized and inflammatory cell infiltration, interstitial vessel wall thickening, random cardiac muscle samples had 3 positive changes, which had similar changes to skeletal muscle, there was 1 positive change from lung sample.

结果：模型C组于免疫注射第3周左右开始出现活动减少，倦怠嗜卧，食欲体重下降等表现，模型B组有14只SD大鼠于免疫注射第4～5周出现上述症状，较C组为轻；模型组肌酶谱中，肌酸激酶、乳酸脱氢酶、谷草转氨酶与对照组比较明显升高，模型C组较模型B组升高更显著；模型组肌电图时限缩短，波幅降低，多相波增多；磁共振检查模型B组和C组选送标本中各有一例有阳性改变，表现为T1MI等或稍低信号，T2MI及STIR序列为高信号，提示肌肉炎症水肿改变；模型B组有11只，模型C组全部大鼠骨骼肌出现病理改变，表现为横纹肌局灶性分布的肌纤维变性炎细胞浸润，间质小血管壁增厚、扩张，随机选送的心肌标本中有3例有阳性改变，表现与骨骼肌相仿，选送的肺标本中有1例有阳性改变，表现为蛋白渗出，炎症细胞浸润和小血管改变。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。