大改变

Rat glioma cell C6 was infected by the deficiency recombinant retrovirus (named C6TK). The sensitivity of C6TK cells to GCV or ACV increased 450 or 10 times than that of C6 cells respectively. Rat C6 glioma model was successfully built, and SD rats inoculated intracerebrally C6TK cells had normal gliomas. The average survival periods of the rats were about 15 days. However, after treated with ACV, the growth of C6TK tumors obviously regressed, and the survival periods extended to 57.8±8.1 days, especially in rats injected with mixed PA317TK and C6 cells or in situ PA317TK cells, which the tumors nearly disappeared after ACV administration and the survival periods extended to over 120 or 71.4±36.1 days respectively, P＜0.001.

结果：构建了带有HSV-tk基因的反转录病毒载体GINaTK，应用脂质体转移方法将GINaTK导入反转录病毒包装细胞PA317，成为产病毒细胞PA317TK，用带有HSV-tk基因的复制缺陷型反转录病毒感染C6细胞，命名为C6TK细胞，对GCV和ACV的敏感性分别高于亲本450倍和10倍；成功地建立了大鼠脑胶质瘤模型，并证实转染细胞C6TK的成瘤效应未改变，存活期约为15天；而经ACV治疗后，含有C6TK细胞的肿瘤生长明显被抑制，大鼠生存期延长为57.8±8.07天，尤其是采用PA317TK细胞混和治疗组和原位治疗组，肿瘤基本消失，大鼠生存期显著延长，混和治疗组存活120天以上，原位治疗组存活至71.4±36.1天。t检验，P值均小于0.001。

Results: GINaTK retroviral vector containing HSV-tk was constructed and transduced into PA317 retrovirus packaging cell by lipofectin (named PA317TK). Rat glioma cell C6 was infected by the deficiency recombinant retrovirus (named C6TK). The sensitivity of C6TK cells to GCV or ACV increased 450 or 10 times than that of C6 cells respectively. Rat C6 glioma model was successfully built, and SD rats inoculated intracerebrally C6TK cells had normal gliomas. The average survival periods of the rats were about 15 days. However, after treated with ACV, the growth of C6TK tumors obviously regressed, and the survival periods extended to 57.8±8.1 days, especially in rats injected with mixed PA317TK and C6 cells or in situ PA317TK cells, which the tumors nearly disappeared after ACV administration and the survival periods extended to over 120 or 71.4±36.1 days respectively, P ＜0.001.

结果：构建了带有HSV-tk基因的反转录病毒载体GINaTK，应用脂质体转移方法将GINaTK导入反转录病毒包装细胞PA317，成为产病毒细胞PA317TK，用带有HSV-tk基因的复制缺陷型反转录病毒感染C6细胞，命名为C6TK细胞，对GCV和ACV的敏感性分别高于亲本450倍和10倍；成功地建立了大鼠脑胶质瘤模型，并证实转染细胞C6TK的成瘤效应未改变，存活期约为15天；而经ACV治疗后，含有C6TK细胞的肿瘤生长明显被抑制，大鼠生存期延长为57.8±8.07天，尤其是采用PA317TK细胞混和治疗组和原位治疗组，肿瘤基本消失，大鼠生存期显著延长，混和治疗组存活120天以上，原位治疗组存活至71.4±36.1天。t检验，P值均小于0.001。

Methods: The changes of lung pathology in newborn rats was observed after LPS was injected intraperitoneally.

腹腔注射LPS致新生大鼠肺损伤的病理改变，应用免疫组化和RT -PCR的方法分别检测肺组织MMP - 2蛋白和t-PA ，PAI- 1mRNA的表达改变。

For example,when the camera moves from a sunlit to a shaded area within a shot ,it is often necessary to change exposure. The iris diaphragm of the lens can be changed,but this would change the depth of field and may be more noticeable than shutting down the variable shutter .

例如，一次摄像中当相机把镜头从阳光充足的地方移动到阴暗的地方时，必然需要改变曝光大小，镜头的可变光圈可以改变，但可能会影响景深，回比你关闭可变快门影响效果更大。

To change the shape of by applying lath and plaster or boarding.

改变形状用板条，灰泥或大木板来改变的形状

To change the shape ofby applying lath and plaster or boarding.

改变形状用板条，灰泥或大木板来改变的形状

The results indicate that, in either single or double blowing, larger erosion rate is mainly found in the slag line and near purging plug area of ladle lining; erosion is accelerated when gas flow rate increases; the erosion rate of ladle lining with double blowing is larger than that of single blowing; erosion rate distribution is obviously different as gas flow rate increases; in heavy blowing, serious erosion centers around slag line area and expands gradually outwards; as purging plug gets close to lining wall, malconformation of erosion rate in ladle lining becomes more prominent and the erosion rate of lining near purging plug increases.

结果表明，无论单吹还是复吹，较大的蚀损速率主要分布在钢包渣线及近透气砖区域，吹气量的增大会加速钢包内衬的蚀损。采用复吹时，钢包内衬的蚀损较单吹时更快。随着吹气参数的改变，钢包内衬蚀损速率的分布状况差异明显。大吹气量下，较大的蚀损集中在渣线区域且呈环向扩展；透气砖安装距包壁越近，钢包内衬蚀损不均衡性也越突出，近透气砖内衬蚀损加重。

Methods: Mixed bacterium was used for making salpingitis caused sterility rats model, treated by different doses of TLF contrasted with Fuyankang , the pathology and morphallaxis of oviduct with Light and Transmission electron microscope was observed, the content of IL-2、IL-6 in serum and oviduct was checked by ELISA method, pregnant results also was traced.

用"混合菌接种法"制作大鼠慢性输卵管炎性不孕动物模型，以不同剂量的通络助孕方和对照药妇炎康对模型大鼠治疗后，观察光镜和电镜下输卵管病理形态学改变情况，用ELISA法检测血清和输卵管组织中IL-2、IL-6含量，并观察大鼠受孕情况。

The corresponding change of the blood flow of the ophthalmic arteries takes place when the internal carotid artery stricture lead to acute cerebral ischemia. The Vmean, Vmax, RI, PI of the ophthalmia artery showed significant reduce, but the change of Vmin was no difference.

当颈内动脉狭窄引起脑缺血时，眼动脉与脑动脉发生相似的血流动力学改变，眼动脉的平均血流速度、最大血流速度、阻力指数、搏动指数较前均明显降低，而最小血流速度不发生明显改变。

The experimental result shows that the projected area of wind direction perpendicular will change with the different angles of flat panel and has more influences on air flow-field of ordinate axis.

经实验发现，因集热板角度改变，使得与风向垂直面之导流板投影面积改变，对於纵轴之空气流场影响较大。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。