多骨的

As tail-suspended rats model can form an abnormal model of lipid metabolism as well as bone metabolism, We added three levels offish oil to the diets of rats of this model to determine the effects of n-3 polyunsaturated fatty acids on lipid and bone metabolism, so as to determine the interaction of these two chronic diseases and clarify the mechanism of these diseases.

尾部悬吊模拟失重大鼠模型能够同时造成大鼠脂质代谢与骨代谢失调，而应用此模型进行脂质代谢与骨代谢失调相关关系及膳食(n-3)多不饱和脂肪酸干预对模拟失重条件下脂质与骨代谢的影响的研究尚未见报道。本实验通过添加以鱼油为来源的不同剂量的(n-3)多不饱和脂肪酸作为膳食干预手段，研究其对模拟失重条件下构建的大鼠脂质代谢与骨代谢异常的调节作用，以加强对脂质代谢与骨代谢相互作用生理进程的深入了解为目标，为阐明中老年人群慢性疾病并发或多发的生理生化机制提供一定的实验依据。

There was a high risk of thin cement mantle in Gruen zones 1,2 and 5 in the anteriorposterior plane and in Gruen zones 8,9 and 12 in the lateral plane.Typical areas of radiolucency were identified in Gruen zones 2～5 and 10～12 in the middledistal of the stem.Voids appeared in Gruen zones 3～5 and 10～12 in the distal of the stem.

结果(1)按Barrack标准，骨水泥质量A级87.1%，B级11.8%，C级1.1%，无D级骨水泥；(2)按Gruen分区，骨水泥鞘厚度 mm区域多见于正位片上1、2、5区和侧位片上8、9、12区；透亮带多位于假体中远端的2～5区和10～12区；气泡影出现在假体远端的3～5区和10～12区。

There was a high risk of thin cement mantle in Gruen zones 1,2 and 5 in the anterior-posterior plane and in Gruen zones 8,9 and 12 in the lateral plane. Typical areas of radiolucency were identified in Gruen zones 25 and 1012 in the middle-distal of the stem. Voids appeared in Gruen zones 35 and 1012 in the distal of the stem.

结果］(1)按Barrack标准，骨水泥质量A级87.1%，B级11.8%，C级1.1%，无D级骨水泥；(2)按Gruen分区，骨水泥鞘厚度＜2mm区域多见于正位片上1、2、5区和侧位片上8、9、12区；透亮带多位于假体中远端的2~5区和10~12区；气泡影出现在假体远端的3~5区和10~12区。

According to our results, since BaP increased the expression of COX2 and pp38 to reduce the osteoblast differentiation and this phenomenon can reverse by treatment of NS398, but not by treatment of SB203580, suggesting that COX2 activation plays an important role on osteoblast differentiation. Adlay could increase osteoblast differentiation, in co-culture system, and reverse the osteoporotic status in mice, would be a helpful healthy food for enhance osteoblast differentiation. However, about, TBT, we didn't have enough data to support its effect on the differentiation of osteoblast or osteoclast.

所以依据我们所做出的实验结果，虽然苯骈芘会增加COX2和pp38的蛋白表现，但造骨细胞的分化作用仍下降，但若同时添加NS398，则分化能力有恢复现象，这或许可推测说COX2活性在抑制造骨细胞分化上扮演了一个重要角色；薏苡仁在共同细胞培养可以增加造骨细胞的分化作用，且对骨质疏松状态的小鼠具有缓解的能力，因此或许是能促进造骨细胞分化的营养食品；至於三丁基锡由於实验的数据不多，推测其或许并不影响造骨细胞和破骨细胞的分化作用。

We present a retrospective clinical study of 23 patients with MTSUTR treated by anterior decompression, strut autografting, posterior instrumentation, and fusion.

目的 上胸椎多节段脊柱结核文献中少有报道，我们对23例上胸椎多节段脊柱结核进行了前路减压自体骨支撑植骨、后路内固定，前后方融合，并对其进行了回顾性临床研究，以平价这一手术方法对上胸椎多节段脊柱结核的临床疗效。

Defects implanted with TGF-β/BMP in animals killed at 16 weeks showed histologically new lamellar and woven bone which was formed in continuity with the cut ends of the cortex. Medullar canal was recanalized and contained marrow elements with normal appearance.

术后第12～16周3组缺损区完全性修复，其密度近正常骨，可见皮质骨和骨髓腔结构。16周时TGF-β和TGF-β/BMP组骨缺损区周围外骨痂少，而BMP组中仍可见较多尚未完全吸收的外骨痂。

The function of bone resorption by MGC was examined with coculture of bone slice and MGC. The resorption lacunae were examined with a scanning electron microscope. The activity of acid phosphatase in MGC was observed by Gomori stain. Results: MGCs showed multinuclei (more than 20 nuclei), irriegular peripheral cytoplasm with pseudopodial projections, vacuoles were seen in the cytoplasm.

用倒置相差显微镜观察体外培养的多核巨细胞的一般形态及降钙素对它的影响；用骨片与多核巨细胞共同培养法观察多核巨细胞的体外骨吸收功能，用扫描电镜观察骨吸收陷窝，Gomori染色观察多核巨细胞的酸性磷酸酶活性。

The pseudopodial movement could be inhibited by calcitonin (100 μg*L－1). Lacuna formation could be found in cocultured specimens. There were fibrils on the walls of lacunae. The MGCs demonstrated positive reaction with Gomori stain. Conclusion: The results demonstrated that MGCs had the same morphological features and the bone resorption ability as the osteoclasts.

结果：倒置相差显微镜下可见多核巨细胞胞核较多（20个以上），胞浆周边不规则，有伪足样突起；胞浆内可见较多大小不等的空泡；降钙素（100 μg*L－1）可抑制多核巨细胞的伪足样运动；多核巨细胞与灭活的骨片共同培养时可见骨吸收陷窝形成，扫描电镜下可见吸收陷窝底面有原纤维；Gomori染色时可见多核巨细胞的酸性磷酸酶呈阳性。

Histopathological observation: 4 weeks after operation, diffuse presence of empty lacunae and pyknotic nuclei of osteocytes were found in the trabeculae, and few newborn micrangium could been seen in group A; lots of empty lacunae and a small quantity of newborn micrangium could been found in group B; and large amounts of osteoblats and newborn micrangium were found around the necrosis regions in groups C and D.

组织病理学观察，术后4周A组见空骨陷窝，无新生毛细血管；B组空骨陷窝较多，有少量新生毛细血管：C、D组空骨陷窝减少，坏死区有大量增生活跃的成骨细胞及新生毛细血管。D组空骨陷窝阳性数及微血管密度分别为19.30 1.52和7.08 1.09，与其他组比较差异有统计学意义（P〈0.05）。

After culture on coverslips and bovine cortical bone slices for 1, 3, 5, and 7 days, morphologic features, TRAP staining and bone absorptive lacunae were observed to evaluate osteoclast cells.

结果显示，当培养细胞在RANKL和M-CSF联合作用下，TRAP阳性染色的单核和多核破骨细胞可在3天内迅速形成，骨片吸收实验显示骨片上出现典型的骨吸收征象；在M-CSF(50ng/ml)协同作用下，RANKL呈剂量依赖型诱导TRAP阳性破骨细胞生成。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。