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in order to establish a rapid, sensitive and specific detection method for identification of four kinds of food-borne pathogenic bacteria including salmonellla spp, listeria monocytogenes, staphylococcus aureus and bacillus cereus by using multiplex pcr, according to invasion protein a geneof salmonellla spp, transcriptional regulatory protein gene of listeria monocytogenes, autolysin gene ofstaphylococcus aureus and gyrase b subunit gene of bacillus cereus, four pairs of specific primers were designed for multiplex pcr amplification.

小 摘要:为建立一种利用多重pcr技术检测和鉴定沙门氏菌、单核细胞增生李斯特菌、金黄色葡萄球菌和蜡样芽孢杆菌的方法,根据沙门氏菌侵袭力蛋白a基因、单核细胞增生李斯特菌蛋白转录调控基因、金黄色葡萄球菌自溶素基因、蜡样芽孢杆菌的促旋酶b亚单位基因设计引物,进行多重pcr扩增,并对多重pcr反应体系进行优化。

Based on the histogram multifractal spectrum estimation, a kernel density estimation of multifractal spectrum is suggested, and moreover a edge dection method by multifractal spectrum is present, simulation results show that image edges detecting through this method have good global and local character, and are more finner.

多重分形频谱直方图估计方法的基础上,提出了一种基于核估计的多重分形频谱估计方法,同时提出了一种基于多重分形频谱分析的图象边缘检测方法,利用该方法所检测出的图象边缘具有良好的全局性和局部性,也就是说它能够检测出比较精细的图象边缘; 4。

Focused on a resonance-grounded power system,a new method of selecting faulted line based on multifractal spectrum of the zero-sequence current is presented.

系统阐述了多重分形和多重分形谱的小波局部极大模计算方法,提出了利用多重分形谱对线路的零序电流进行分析,根据谱图进行判断故障线路的新方法。

Results: The MBCs of ethanol, glutaraldehyde disinfectant, ShiKang disinfectant and quaternary ammonium disinfectant against multidrug-resistant Acinetobacter baumannii were 45%, 10000 mg/L, 6.3~12.5 mg/L and 100 mg/L, and the MBCs were 40%, 5000 mg/L, 3.1~6.3 mg/L and 50 mg/L Conclusion: MBC and MIC could represent the sensitive dose of multidrug-resistant Acmetoborter baumannu to disinfectants, and eight strains of multidrug-resistant Acinetobacter baumannii have certain resistance to some common disinfectants.

结果:乙醇、戊二醛消毒液、施康消毒液、季铵盐消毒剂对多重耐药鲍曼不动杆菌的MBC值分别为45%、10000 mg/L、6.3~12.5 mg/L、100 mg/L;MIC值分别为40%、5000 mg/L、3.1~6.3 mg/L、50 mg/L、50 mg/L。结论:MBC值和MIC值能基本反应多重耐药鲍曼不动杆菌对消毒剂的敏感剂量,8株多重耐药鲍曼不动杆菌对部分消毒剂产生一定程度的耐药性。

The life, which shuttles back and forth on the edge of multiple cultures, grants Amy Tan multiple visual angles. These visual multiple angles proceed to decide her openness and mobility of her literary creation.

穿梭于多重文化边缘之上的生活赋予了这位作家以多重的视角,这种多重的视角进而决定了其文学创作的开放性与流动性。

The question is this: Which is the wisest course for the Latter-day Saints to pursue—to continue to attempt to practice plural marriage, with the laws of the nation against it and the opposition of sixty millions of people, and at the cost of the confiscation and loss of all the Temples, and the stopping of all the ordinances therein, both for the living and the dead, and the imprisonment of the First Presidency and Twelve and the heads of families in the Church, and the confiscation of personal property of the people (all of which of themselves would stop the practice); or, after doing and suffering what we have through our adherence to this principle to cease the practice and submit to the law, and through doing so leave the Prophets, Apostles and fathers at home, so that they can instruct the people and attend to the duties of the Church, and also leave the Temples in the hands of the Saints, so that they can attend to the ordinances of the Gospel, both for the living and the dead?

这个问题是这样子的:哪一个才是后期圣徒最明智的方向?继续尝试多重婚姻,即使在国家法律的抵挡,六千万人民的反对,承受财产被没收和失去所有的圣殿,和所有在其中的给活人和死人仪式,还有总会会长团、十二使徒和教会家庭的领袖们的被关的情况之下(在这些情况之下仍然会停止继续多重婚姻)?或者,在因我们固执於这个原则的实行并为此受苦之后停止多重婚姻并且服从法律,这样子让我们的先知、使徒、和父亲们留在家里,所以他们可以教导人群参与教会圣工职责并让圣殿留在圣徒的手中,所以他们可以参与给活人和死人的福音的仪式?

Then the spatial index is constructed which includes not only the external approximate expression but also interior approximate expression of spatial object and is extended from tranditional index only with external approximate expression. The index structures of MR-tree and MRD-tree based on multi-approximate expression are set up with the prototype index of R-tree, and the relevant algorithms about the index of insertion, deletion and search are provided. Then, the efficiencies of constructing index based on the multi-approximate expression and window range query are analysised and compared. In addition, the several algorithms and efficiency of soving the maximum enclosed circle and maximum enclosed rectangle are discussed and analyzed during constructing index based on multi-approximate expression.

首先,论文讨论了空间数据的特点、空间数据库的关键技术以及空间对象间的拓扑、距离以及方向关系,分析了空间对象的外部和内部近似表达,然后将传统索引中只利用空间对象的外部近似扩展为既有空间对象的外部近似又有空间对象的内部近似,即以空间对象的多重近似来构建索引,并以R-tree为索引原型构建基于多重近似的MR-tree和MRD-tree索引结构,然后给出了相关索引的插入、删除及查找算法,并通过实验比较分析了基于多重近似索引与基于外部近似索引的构建效率及窗口范围查询效率。

A plastic model was proposed which develops from the combination of boundary surface theory and multi-shear mechanism plastic theory. This model avoids the uncertainty and complication associated with the revision of Mansing's rule when a multi-shear mechanism plastic model is used, and is able to simulate the rotation of principle stress axes during seismic activities and the large shear deformation during liquefaction.

将边界面理论和多重剪切机构塑性理论结合起来并加以改进建立了多重剪切机构边界面塑性模型,避免了多重剪切机构塑性模型通过修正系统修正曼辛准则时的不确定性、复杂性;且能模拟地震时主应力轴的偏转和液化时剪切大变形。

Significantly different scalings of the mean IMF multiplicities with neutron,charged-particle,light charged-particle multiplicities,and Zbound are observed for the two reactions.

观察到两个反应系统中,中等质量碎片多重性、中子多重性、荷电粒子多重性与轻荷电粒子多重性之间的关联存在着明显的差别。

Sighficantly different scalings of the mean IMF multiplicties with neutron, charged-particle, light charged-particle multiplicities, and Z_bound are observed for the two reactions.

观察到两个反应系统中,中等质量碎片多重性、中子多重性、荷电粒子多重性与轻荷电粒子多重性之间的关联存在着明显的差别。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。