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Methods:The peptide sequences of human CYP1A2 were analyzed according to its bioinformatics, and synthesized on the basis of hydrophilicity, antigenicity, accessibility and flexsibility. The synthesized peptides were crosslinked with keyhole limpet hemocyanin, which was used to immunize rabbits for production of Ab.

利用生物信息学方法分析CYP1A2蛋白的序列,根据亲水性、抗原性、柔韧性及表面性等指标选择多肽序列,合成CYP1A2多肽,与载体蛋白钥孔戚血蓝素(Keyhole limpet hemocyanin, KLH)偶联,免疫日本大耳白兔制备抗CYP1A2抗体。

Methods PIWIL polypeptide was synthesized and conjugated to Keyhole limpet hemocyanin as an immunogen. Then PIWIL-KLH conjugations were injected into rabbits subcutaneously to produce polyclonal antibodies.

合成特异性PIWIL多肽,以马来酰胺活化匙孔血蓝蛋白作为载体构建多肽免疫原,通过致敏大白兔,制备兔抗人PIWIL多克隆抗体,用亲和层析法纯化抗体。

Methods ①Human peptides of CYP450 enzymes and their proteinic sequence were analyzed by means of bioinformatics, and certain sequences were chosen and synthesized according to four indexes (Hydrophilicity Plot, Flexible Regions, Antigenic Index and Surface Probability Plot) after homology analysis by software Blast. The synthesized peptides were conjugated to keyhole limpet hemocyanin to increase antigenicity.

①利用生物信息学方法分析CYP1A2、CYP3A4及CYP4A11蛋白的序列,利用DNAstar软件中蛋白质的亲水性结构、柔韧区、抗原指数及表面概率结构指标选择多肽序列,应用Blast软件对所选序列进行同源性分析并合成多肽,与载体蛋白钥孔戚血蓝素偶联,免疫大耳白兔制备抗CYP1A2、3A4及4A11抗体。

Methods AGOs polypeptide was synthesized by chemical method, and conjugated to Keyhole limpet hemocyanin as immunogen. Then AGO-KLH conjugations were injected into rabbits subcutaneously to produce polyclonal antibodies.

合成特异性AGO多肽,以马来酰胺活化匙孔血蓝蛋白(keyhole limpet hemocyanin, KLH)作为载体构建多肽免疫原,通过致敏大白兔,制备系列兔抗人AGO多克隆抗体,然后用亲和层析法纯化抗体。

The formula of instant powder with sheep bone collagen polypeptides for children was studied. Several powdered vegetables and oat flour were added into the powdered milk and pulse flour too.

以奶粉、豆粉为基料,在添加羊骨胶原多肽的同时,适当添加各种蔬菜粉、燕麦粉及其他辅料,生产儿童专用型羊骨胶原多肽速溶粉。

In order to elucidate the relationship between the structural features of leginsulin gene in legume plants and their phylogenetic significance,we have cloned the cDNA sequence of leginsulin gene from radicles of broad bean via RT-PCR techniques according to the leginsulin gene sequence we previously obtained from soybean.The cloned cDNA encoded for a precursor protein consisting of the signal peptide,mature leginsulin and an additional 45 amino acids of another polypeptide.

为探明豆科植物中豆类胰岛素基因的结构特征与进化关系,在已获得大豆豆类胰岛素基因的基础上,以蚕豆种子胚根mRNA为材料,采用RT-PCR技术,克隆了蚕豆豆类胰岛素基因的cDNA序列,编码的前体多肽包括信号肽、成熟型豆类胰岛素及另一多肽的45个氨基酸残基。

The 57 kD precursor, 37-39 kD acidic subunit and 22-23 kD basic subunit of glutelin and the 13 kD prolamine could be showed clearly.

各种水稻能够显示清晰的蛋白组分条带主要有:谷蛋白37~39 kD酸性亚基、22~23 kD碱性亚基、谷蛋白57 kD前体多肽和13 kD醇溶蛋白多肽

The protein deposited is beta-amyloid peptides derived from beta-amyloid precursor protein, similar to the peptided found in senile plaques in neuropil of normal aging brains and Alzheimer disease brains.

沉积的蛋白是源自β-淀粉前体蛋白的β-淀粉多肽,类似于在正常老化大脑和阿尔茨海默病的大脑中神经毡、老年斑中发现的多肽

Our company main products of rice starch and rice protein peptide powder, is a handful of domestic production of rice starch and rice powder protein, peptide, one of the manufacturers.

本公司主要产品为大米淀粉和大米蛋白多肽粉,是目前国内少数几个生产大米淀粉和大米蛋白多肽粉的厂家之一。

However, these algorithms could produce a significant number of incorrect peptide identifications.

然而,它们仍存在大量错误的多肽鉴定。我们提出一种新的串联质谱识别多肽序列的计量算法。

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