多肽

The invention provides a method when alkaline protease hydrolyses from big molecular collagen into minor polypeptides, free amino acids in enzymatic hydrolysate increase gradually, a positive correlation is shown between increased amount and hydrolysis degree, so the hydrolysis degree can be calculated by detecting total amino acids or a certain kind of amino acid content in enzymolysis liquid.

本方法是利用碱性蛋白酶水解大分子胶原蛋白生成小分子多肽的同时，酶解产物里游离氨基酸含量逐渐增加，其增加的量与水解度大小呈正相关，因此，通过检测酶解液中氨基酸总量或某一种氨基酸含量可计算出水解度的大小。

Within the immunised host, they mimic the effect of live attenuated viral vaccines when continuously expressing the polypeptide in question.

在受免个体体内，DNA疫苗能否持续表达多肽尚有疑问，但其能诱导类似弱毒苗的免疫效应。

Primary structure : a polymer, the sequence of amino acids and any interchain and intrachain In disulfide bonds of a protein.

蛋白质分子多肽链中氨基酸的排列顺序称为蛋白质的一级结构

Protein disulfide isomerase is one of main retention proteins in ER, which is an abundant protein in luman of the ER especially in secretory cells.

蛋白质二硫键异构酶是内质网腔内的主要驻留蛋白之一，在分泌细胞的内质网腔中含量非常丰富，其主要功能是催化细胞内分泌蛋白和膜蛋白新生多肽的二硫键形成。

METHODS: Two polypeptides named peptide 1 and 2 were synthesized based on the bioinformatics analysis of the sequence of hMR-1 by using software TMHMM and DNAStar, then coupled with keyhole limpet hemocyanin for immunization.

TMHMM、DNAstar等软件分析hMR-1蛋白序列，选取2段优势抗原序列合成多肽并与钥孔戚血蓝素偶联，混合后免疫家兔。

To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin, and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay, the antibody was purified with rProtein A sepharose fast flow and dephosphorylated antigen membrane. The antibody titrate reached 1:10 000 after purification and its special property was enhanced with absorption treatment of dephosphorylated antigen membrane. In addition, we used rabbit anti-PRAS40 antibody and the phosphorylated PRAS40 (Ser183) antibody to detect PRAS40 expression in several cell lines, including the normal cells HL7702, HEK293, tumor cells HepG2, A549 and S180. There were no quite difference among these cells; otherwise, we observed the decreased phosphorylation level of Ser183 after amino acid withdrawal treatment.

为了制备PRAS40(Ser183)磷酸化多克隆抗体，本实验通过蛋白疏水性抗原性分析设计多肽抗原，用其免疫家兔获得抗血清， ELISA检测其效价为1：10 000； Western blotting法检测发现，通过rProtein A Sepharose亲和层析纯化并经非磷酸化的抗原条吸附处理后的抗体可以明显提高磷酸化抗体的特异性；用PRAS40抗体及PRAS40(Ser183)磷酸化抗体对正常细胞HL7702、HEK293及肿瘤细胞HepG2、A549、S180的检测显示：磷酸化的Ser183在不同细胞中表达差异不显著，而在经细胞饥饿处理的HEK293细胞中却明显观察到了S183磷酸化水平随氨基酸含量降低而减弱的现象。

In order to overcome defect of traditional koumiss, the technology for the koumiss processed from juice mixed with milk peptides hydrolyzed by protein enzyme was studied by orthogonal methods and contrast tests.

为克服传统乳酒缺陷，该文采用蛋白酶水解法及酒精酵母乳酸菌混合发酵法研究了牛乳蛋白多肽复合果汁乳酒的生产工艺。

AIM: A new hepatocyte stimulate peptide from shark liver was purified and characterized.

目的：从鲨鱼肝脏中分离纯化得到了一种鲨鱼肝刺激多肽。

Objectives:To explore the effect of Shahepatide,an a ctive polypeptide extracted from shark liver,on secretion of IFN-γ and TNP-β from human periperal blood mononuclear cellsand to explore its liver-pr otective effect in mice with immune liver injury.

目的：探讨从海洋生物鲨鱼肝纯化获得的一种活性多肽对人外周血单核细胞分泌IFN-γ及TNF-β的影响，以及对小鼠免疫性肝损伤的保护作用。

We found that the volume of nucleophile RO〓- can effect the hydrolysis of carboxyrster, the nucleophilicity of RO〓- and the Lewis acidity of metal macrocycle also effect the carboxyester hydrolysis.

β淀粉样多肽是阿尔茨海默病病人脑中不溶性淀粉样沉淀的主要组成部分，且β-肽在人体中以何种形式存在主要取决于其N-端1-28残基[β-（1-28）]的构型。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。