多肽

The values obtained in this paper are helpful for deeply understanding the structure and stability of theα- andβ-peptide conformers.

本文的计算结果对深入研究α-和β-多肽构象的空间结构和稳定性有重要参考价值。

The hydrogen bonding energies for the twentyα- andβ-peptide conformers were evaluated by the substitution method.

应用取代法估算了这20个α-和β-多肽构象中不同类型的N-H…O=C分子内氢键键能。

After metal exposure (at PFD 35 mmol m-2 s-1 and temperature 21±1 8℃), algal samples were withdrawn, separated from the medium by filtration on a Whatman GF/C filter, washed with deionised water and extracted for thiol peptide analysis.

在金属接触（在光强35浓度 m-2 s-1和温度21 ±1 8℃），藻类样品被撤回，脱离介质过滤的瓦特曼石墨/碳过滤器，洗涤水和提取deionised为硫醇多肽分析。

Methods: Encapsulate synthesized MG7 mimotope peptide into nanoemulsion using magnetic and ultrasonic technique. Dialyse method was used to sterilize, HPLC to determine encapsulation efficiency.

人工合成胃癌MG7-Ag的模拟表位多肽，用磁力超声法将其包封于纳米乳剂中，通过透析纯化，HPLC检测其包封效率。

First, the significant of co-factor InsP6 and center water in the mechanism of auxins are explored by docking; second, the experiments that compared with the docking rusults of two situations involving fully rigid and selective flexible of active residue of the receptor TIR1 illuminate that selective flexibility docking by AutoDock4 reports more rational results, so that, AutoDock4 dockings are implemented with TIR1-Auxins; third, AutoDock4 docking by TIR1-Auxins-Aux/IAA illuminates that auxin as a molecular glue enhances the interaction between TIR1 receptor and Aux/IAA substrate by the weak interactions, such as hydrogen bond and hydrophobic interaction, furthermore, the weak interactions between receptor protein and ligands greatly influence on auxin activity of auxin ligands.

首先， 通过分子对接计算研究辅酶InsP6以及中心水分子在生长素反应中的重要作用；其次，比较受体大分子完全刚性以及活性残基部分柔性的两种情况下的分子对接结果，说明AutoDock4实现了受体分子活性残基的部分柔性而使对接结果更加合理，进而使用AutoDock4方法对TIR1-Auxins体系进行对接计算；最后，对TIR1-Auxins-Aux/IAA体系进行分子对接计算，结果表明，生长素配体分子作为&分子胶水&直接与受体大分子TIR1以及底物多肽Aux/IAA形成强的弱相互作用，如氢键作用、疏水相互作用，促进了受体TIR1与Aux/IAA底物之间的结合，进而说明氢键作用和疏水相互作用等弱相互作用对于生长素分子的活性具有很大的影响。

Utilizing the reversed-phase high performance liquid chromatogram to carry out further purification to the deer placenta polypeptide, the best purifying condition of separation is: Chromatogram column Hypersil ODS C18 (4.6*250mm), A liquid 0.1% TFA /ACN, B liquid 0.1% TFA / water, in 30min10% A ～50% A and 90% B ～50% B linear gradient elutes, velocity of flow 1.0mL/min, temperature 30℃, detecting wavelength 214nm.

利用反相液相色谱技术对鹿胎盘多肽进一步纯化，最佳分离纯化条件为：色谱柱 Hypersil ODS C18(4.6×250mm )，流动相 A 液0.1% TFA/乙睛，B 液0.1%TFA/水，30min 内10%A ～50%A ，90%B ～50%B 线性梯度洗脱，流速1.0mL/min ，温度30℃，检测波长214nm 。

The present application also features methods of eluting the purified polypeptide as well as the incorporation of the methods within a purification train.

本申请的特征还在于洗脱被纯化多肽的方法及纯化系列中该方法的并入。

Objective To construct the eucaryotic recombinant plasmid of pYES2/LactoferricinB and express in yeast of S.cerevisiae and preliminary verify the antibacterial activity.

目的构建牛乳铁多肽的真核表达质粒pYES2／Lactoferricin B，实现其在酿酒酵母S.cerevisiae中的表达，初步检测其不同变异的体外抗菌活性。

Objective To construct the eucaryotic recombinant plasmid of pYES2/LactoferricinB expressing in yeast of S. cerevisiae, of which the expressed protein antibacterial activity was verified in preliminary.

目的 构建牛乳铁多肽的真核表达质粒pYES2/Lactoferricin B，实现其在酿酒酵母S.cerevisiae中的表达，并初步检测其不同变异的体外抗菌活性。

Furin is also involved in many diseases such as the activation of viral glycoproteins and bacterial exotoxins and the metastasis of cancer.

多肽或蛋白质以前体形式合成后，需要前体加工酶的剪切加工才能获得完全的生物活性。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。