多肽

To synthesize the octreotide, Phe-Leu-Glu-Glu-Leu and C_(15)H_(31)-CO-Lys-Thr -Thr-Lys-Ser peptides, the strategies are as followings: Fmoc- group as protective group for α-NH_2; TBTU, DIEA and HOBt as coupling reagent; and ethanedithiol -phenol - thioanisole - H_2O - TFA ( 2.5:5:5:5:82.5 , V/V ) as cleaving solution.

采用Fmoc固相合成法，以苯并三哗四甲基脲四氟硼酸盐、二异丙基乙胺和1-羟基苯骈三氮唑为缩合剂；苯酚—乙二硫醇—苯甲硫醚—水—三氟乙酸(5：5：2.5：5：82.5，V/V)为切割试剂，合成了奥曲肽、Phe-Leu-Glu-Glu-Leu及C_(15)H_(31)-CO-Lys-Thr-Thr-Lys-Ser三种小分子多肽，并对奥曲肽的合成规模进行了放大。

The results showed that the higher peptides yield was 38.96mg/g on the conditions of 1% brown sugar power,initial pH7.5,temperature 30 ℃,rotational speed 180 r/min and the substrate 7%,which was fermented 48 h.The higher yield of free amino acids was 0.344% when ferme...

结果表明，发酵培养基碳源为红糖粉添加量1%，发酵初始pH 7.5，发酵温度30℃，转速180 r/min以及底物浓度7%，发酵48 h时多肽含量达到38.96 mg/g，当发酵时间持续到72 h可得到较多游离氨基酸，含量为0.344%发酵48 h发酵液的香味较好。

Liver disease ; alcoholic ; Low molecular weight polypeptide7; Carbonyl stress ; Polymerase chain reaction ; Fluorogenic quantitative

酒精性肝病；低分子量多肽7；羰基应激；聚合酶链反应；荧光定量

Because of no signal from N-terminal protein sequencing for 43kDa protein directly, a resulting 18kDa peptide from the partial cleavage of 43kDa protein by BrCN had its N-terminal sequences of AFTFKK determined by N-terminal protein sequencing assay. Then, the 43kDa protein was convinced to the enzyme of 3phosphate glycerate kinase (PGK1) by searching YPD.

由于43kDa蛋白直接进行蛋白质氨基端序列测定时没有信号，因而用溴化氰部分化学裂解43kDa蛋白，得到的18 kDa多肽经蛋白质氨基端序列测定，得到其氨基端前6个氨基酸残基序列为AFTFKK，通过计算机检索YPD，确定43 kDa蛋白是3—磷酸甘油酸激酶（PGK1）。

The results indicate that Ax peptides of the acid subunit of soybean glycinin was most susceptible, and the β bands of β-conglycinin and basic subunit of glycinin was most resistant to digestion.

结果表明，大豆球蛋白酸性亚基的Ax多肽链最容易被水解，所有的酶对其均有作用，而碱性亚基和大豆伴球蛋白的β亚基最难被水解。

The study shows that: Kelp contains plenty of taste amino acid, mushrooms contain plenty of guanylic acid, bonito fish is rich in taste inosinic acid, and chicken soup has abundant taste polypeptide and amino acid.

研究表明：海带中存在大量的呈味氨基酸、蘑菇含有大量的鸟甘酸、鲣鱼含有丰富的呈味肌苷酸、鸡汤含有丰富的呈味多肽和氨基酸。

It contains abundant dainty substances as nucleotide, guanylic acid, inosinic acid, polypeptide and amino acid, as well as plenty of vitamin B complex.

其核苷酸、鸟甘酸、肌苷酸及多肽和氨基酸等美味物质异常丰富，还含有大量的维生素B群。

The pathogenic mechanism in halobios caused with various organic contaminations were studied by proteomic techniques including its relative analytical techniques.

研究有机污染源危害海洋生物的致病分子机理。以多维核磁共振法和X光晶体衍射为分析手段，研究重要蛋白质的精细结构和多肽药物分子设计。

By using PCR method, a 1kb fragment was gained from Heliothis amigera nuclear polyhedrosis virus genome. The whole fragment was sequenced by dideoxided chain termination methods and a full open reading frame which encode 299 amino-acids was found. The molecular weight of the peptide is about 35 kilodalton.

用PCR方法从棉铃虫核多角体病毒基因组中扩增到了1kb的DNA片段，用双脱氧链终止银染测序法测定了该片段的全序列，发现它含有一个完整的开放阅读框，编码299个氨基酸残基的多肽，分子量约35KD。

The present invention also discloses the method of applying the polypeptide in treating various diseases, such as malignant tumor, hemopathy, HIV infection, immunological diseases and inflammations.

本发明还公开了此多肽用于治疗多种疾病的方法，如恶性肿瘤，血液病，HIV感染和免疫性疾病和各类炎症等。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。