多肽

The 3rdpassage chondrocytes were divided into blank group, different concentration PAP groups,different concentration glucosaminsalfate groups and were sequently passaged to 4thgeneration. The 2nd passage chondrocytes was contrasted as young cells group. Thechondrocytes of different groups were detected with the methods of histochemistry forS-A-β-gal, and with alcian blue test for the content and constructure of GAG of ECM,immuocytochemistry for typeⅡcollagen and PCNA, MTT assay for proliferation, RT-PCRfor typeⅡcollagen and Aggrecan, flow cytometry for cell life cycle and proliferationindex,by which to observe PAP"s function regarding to the appearance and functional status inthe process of chondrocyte"s cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养，同时以P2代软骨细胞为对照组，进行组化检测老化相关β-半乳糖苷酶，阿力新蓝染色检测胞外基质硫酸GAG含量和结构，MTT比色检测增殖，免疫细胞化检测PCNA和Ⅱ型胶原，RT-PCR检测Ⅱ型胶原、Aggrecan蛋白，流式细胞仪分析细胞周期和增殖指数等方法，对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

In the first part, twelve peptides, including eight conservative amino acid residues in the amino acid sequence of hydrophilic S helix of AOX. were synthesized by solid-phase method. The polypeptide was coupled to a -chymotrypsinogen, and the antibodies were obtained through immunizing rabbit by injecting this complex.

第一部分，我们按照交替氧化酶(Alternative Oxidase，AOX)位于线粒体内膜外侧亲水区S螺旋的氨基酸序列，用固相法合成由12个氨基酸残基组成的多肽，将此多肽与α-牛胰凝乳蛋白酶原A相连，用作半抗原，免疫家兔制备人工合成12肽的抗体。

Objective To isolate new conotoxins of Conus textile from the South China Sea,provide new conopeptides for drug discovery and design, and investigate the effects of living environment on toxin secretion by comparing the compositions of the conotoxins of this species from different areas.

目的从中国南海织锦芋螺中分离新的芋螺毒素，为药物发现、药物设计提供新的活性多肽或前体多肽，比较不同地域织锦芋螺毒素的构成，研究环境对织锦芋螺毒素分泌的影响。

The fragment encoding the polypeptide of wheat MLO protein C terminus located in cytoplast was cloned into the vector pET-30a and expressed in the strain BL21 of E coli.

本研究将编码小麦MLO蛋白羧基端约130个氨基酸多肽的基因片断克隆到表达载体pET-30a中，在大肠杆菌中表达了该多肽的融合蛋白。

Interaction between fusion peptide of influenza virus A and its related antisense peptides was studied. A peptide was screened out with high binding ability to fusion peptide and the dissociation constant was shown at the level of umol/L. In order to quantitatively characterizing the affinity interaction, a system consisted of quartz crystal microbalance biosensor and flow injection analysis was designed and established.

据此初步结果，研究了甲型流感病毒融合肽与其反义肽的相互作用；提出了基于反义肽的组合化学法筛选目的多肽或蛋白质的亲和配基的全新的研究思路，筛选到了与融合肽具有强相互作用的多肽，其与融合肽的解离常数达到umol/L。

We also inserted an Enterokinase recognition site between the hSOD gene and the small peptide gene, then we can cleave away the small peptide from fusion protein easily.

在构建时还在小分子多肽与人SOD的连接区插入肠激酶识别位点，这样可以使小分子多肽能从融合蛋白中切割下来。

Methods PIWIL4 peptide was synthesized using chemical method, and conjugated to Keyhole limpet hemocyanin as immunogen.

方法合成特异性PIWIIA多肽，以马来酰胺活化匙孔血蓝蛋白作为载体构建多肽免疫原，通过致敏大白兔，制备兔抗人PIWIIA多克隆抗体，然后用亲和层析法纯化抗体。

398 Neutral protease applied in technology for preparation of gelatin hydrolyzate was discussed.

水解明胶亦称胶原多肽，是胶原蛋白或明胶经过一定的降解而得到的多肽混合物。

Dialysis experiment and Mass spectrograph analysis showed that peptides with the molecular weight below 5000D were 90%;below 3000D were 44%.

经透析试验和质谱分析证明，活性肽粉中多肽主要集中在5KD以下，占蛋白质总量的90%左右，其中3KD以下多肽含量占44%左右。

The amphioxus GDF8/11 cDNA encodes a polypeptide of 419 amino acid residues that showed high homology with MSTNs of molluscan, teleostean, avian, and mammalian species and GDF11 of mammalian and zebrafish species.

文昌鱼GDF8/11 cDNA编码一个419个氨基酸的前体多肽，这个前体多肽与软体动物、硬骨鱼类、鸟类和哺乳动物的MSTN以及哺乳动物和斑马鱼的GDF11具有高同源性。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。