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The main collagens in skin are type I and III. In this research, total collagens were separated from rat skin by enzymatic digest and acetum methods. The collagens were denatured at 60℃ and digested with trypsin. Characteristic peptides typical for collagen type I and III were identified with high performance liquid chromatography/mass spectrometry.

哺乳动物皮肤真皮中胶原蛋白含量约为70%,主要为是I型、III型胶原蛋白,本实验利用稀酸溶解和酶法提取了大鼠皮肤中的总胶原蛋白,将胶原蛋白粗提品在60℃变性后用胰蛋白酶进行降解,液相色谱/质谱联用法分析了两种胶原蛋白的特征多肽,利用特征多肽比较了不同生长期大鼠皮肤中I型和III型胶原蛋白相对含量。

Physiological significance in the alleviative bioactivity: Polypeptide's physiological efficiency in the organic body not only determined by the compatible acceptor but also polypeptide's structure and concentration.

调节生理活性作用明显:由于多肽在机体内发挥生理作用,除了有其对应的受体外,还取决于多肽的结构和浓度。

The results of this study indicate that when these cyclic peptides self-assemble as either parallel or antiparallel stacking modes, hydrogen bonds could be formed between side chains of Asn, Asp, Gln and Glu.

结果表明,四种环状多肽无论以平行还是以反平行的方式聚集,聚集体中相邻两个环状多肽的侧链之间都能形成氢键。

Aim: To study the amino acid-composition of purified 3 major components(arachin, conarachin I and conarachin II) and the five purified polypeptides. To separate the methionine-rich proteins and analyze their changes of synthesis and degradation during development and germination in peanut seeds.

目的:分析花生球蛋白、伴花生球蛋白Ⅰ和伴花生球蛋白Ⅱ及其组成多肽的氨基酸组成,筛选高甲硫氨酸多肽并研究其在花生种子发育和萌发过程中的合成降解规律。

In chapter 3, we developed a CE-ESI-MS method to analysis mixture peptides (peptide hormones of braine and intestine and bradykinin-related peptides), and applied to analysis of human saliva with satisfactory results.

在第三章,我们采用CE-ESI-MS对由脑肠肽和激肽组成的生物多肽进行了分析研究,并将建立的方法应用于分析人体唾液样品中的多肽

The third passage chondrocytes were divided into blank group, different desity PAP groups, different desity glucosaminsalfate groups which were passaged to 4th generation and contrast to the 2nd passage group. The chondrocytes of different groups were detected with the method of histochemistry for S-A-β-gal,and with alcian blue test for the content and constructure of GAG of ECM, immuocytochemistry for type Ⅱcollagen and PCNA, MTT assay for proliferation, RT-PCR for type Ⅱcollagen and Aggrecan, flow cytometry for cell life cycle and proliferation index,by which to observe PAP's function regarding to the appearance and functional status in the process of chondrocyte's cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养,同时以P2代软骨细胞为对照组,进行组化检测老化相关β-半乳糖苷酶,阿力新蓝染色检测胞外基质硫酸GAG含量和结构,MTT比色检测增殖,免疫细胞化检测PCNA和Ⅱ型胶原,RT-PCR检测Ⅱ型胶原、Aggrecan蛋白,流式细胞仪分析细胞周期和增殖指数等方法,对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

The paper still studied the soluble characteristic of soybean peptides in the acid beverage, and reviewed the color, flavor and stability of the beverage such as apple, haw and orange juice after adding soybean into them and the optimum proportion.

论文研究了大豆多肽在酸性饮料中等电点可溶的特性,研究开发了大豆多肽苹果汁、山楂汁、橙汁等饮料,考察了大豆多肽果汁饮料的色泽、风味、稳定性等指标,并确定出最佳配比方案。

Biological activities of SPThe inhibitory effect of SP on oxidation of lipids and the acitivity of lipoxygenase, the scavenging or quenching effect of SP on free radicals, and the influences of SP on the malonaldehyde content in mice liver juice, liver mitochondria and liver red blood cell were studied. The results showed that SP can inhibit the auto-oxidation of lard and soybean oil at 60 and can inhibit the activity of lipoxygenase significantly; SP also had scavenging effect on hydroxylradicals generated by Fenton system with IC50 value 6.220 mg/ml, under the concentration range of 1 mg/ml-50mg/ml, the scavenging rate of SP to -OH increased strongly when the concentration of SP increased, the maximum scavenging rate was 85.4%.

大豆多肽的生物活性研究研究了大豆多肽抗油脂氧化、抑制脂肪氧合酶及清除自由基的作用,以及对小鼠肝组织匀浆、肝线粒体和红细胞丙二醛含量的影响,结果显示:在60℃时,大豆多肽对猪油与豆油的自动氧化均有一定的抑制作用:对脂肪氧合酶的抑制作用显著:对Fenton体系产生的羟基自由基具有明显的清除作用,其IC_(50)值为6.220mg/ml。

The present application provides methods of purifying polypeptides having a Fc region, for example, antibodies or antibody fusions, by adsorbing the polypeptides to a Fc binding agent, such as, for example, Protein A or Protein G, followed by a wash with a divalent cation salt buffer to remove impurities and subsequent recovery of the adsorbed polypeptides.

本申请提供了通过将具有Fc区的多肽,例如,抗体或抗体融合吸附到Fc结合剂,例如,A蛋白或G蛋白上,然后用二价阳离子盐缓冲液洗涤,以除去杂质,随后回收被吸附的多肽来纯化所述具有Fc区的多肽的方法。

Highly active, small-molecule furin inhibitors are attractive drug candidates to fend off bacterial exotoxins and viral infection. Based on the 22-residue, active Lys fragment of the mung bean trypsin inhibitor, a series of furin inhibitors were designed and synthesized, and their inhibitory activity toward furin and kexin was evaluated using enzyme kinetic analysis. The most potent inhibitor, containing 16 amino acid residues with a Ki value of 2.45×10~(-9) M for furin and of 5.60×10~(-7) M for kexin, was designed by three incremental approaches.

本论文研究活性多肽类似物的设计、制备和功能,分为四个部分,采用多肽固相和液相合成的方法,分别合成了活性多肽绿豆胰蛋白酶抑制剂Lys片段长链16肽、天花粉胰蛋白酶抑制剂、癌胚抗原肽、甜味肽等一系列类似物,并测定了这些类似物的生物活性,有以下新的研究结果:1、furin酶抑制剂的设计、制备和功能;2、芳香氨基酸芳环之间的π-π共轭能部分补偿二硫键的作用;3、癌胚抗原肽Gly4被L-Pro替代可增加与HLA-A2分子的结合;4、肽键对甜味肽保持甜味是必须的。

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