多肽

ABSTRACT Antitumor Activity of Dendritic Cells Activated with gP96 Peptide Complex Department of Thoracic Surgery, Peking University People's Hospital Objectives: To investigate the expression level of gP96 in human lung cancer samples; purify gP96-polypeptide from human and mouse lung adenocarcinoma cell lines; prepare tumor vaccine by in vitro gP96-polypeptide stimulation of monocyte-derived dendritic cells and test its efficiency both in vitro and in mice models.

研究人肺癌组织热休克蛋白gP96—多肽复合物的表达水平；肺癌细胞株来源的肿瘤组织gP96—多肽复合物的提取和鉴定；外周血来源树突状细胞培养和鉴定：将gP96—多肽复合物和DC体外共同孵育制备疫苗，在体外激活T淋巴细胞的能力及在小鼠体内的抑制肿瘤生长作用的研究。

Most of these peptides are amidated at their C-terminals, which is essential for their full activities. The a-amide comes from posttranslational modification that is catalyzed by α-amidase (α-AE or PAM). The amidation reaction is a rate-limiting step in the biosynthesis of regulatory peptides.

大部分活性肽的C-端是酰胺化的，而且α-酰胺基为多肽的完全生物活性所必需；α-酰胺基来自多肽的翻译后加工，由α-酰胺化酶催化完成，是酰胺化多肽生物合成途径中的限述步骤。

The paper also studied the antioxidation capacity of soybean peptides in the food system and non-food system. The results showed that the soybean peptides can prolong the inducing period of the axunge oxygenation and cooperate with hydroxybenzene antioxidant which was more obvious in the food system. In the non-food system, the soybean peptides can control the autooxidation speeds of octadecadienoic acid and diphenolic compound and the effect on the latter was more obvious.

论文对大豆多肽在食物体系与非食物体系中的抗氧化性能做了研究，结果表明：大豆多肽在食物体系中能够延长油脂氧化酸败的诱导期，而且与酚类抗氧化剂协同作用，效果更为明显；在非食物体系中，大豆多肽能够抑制亚油酸的自氧化及邻苯三酚的自氧化速率，其中对后者的抑制作用较前者显著。

The present invention discloses a new kind of polypeptide, flavoprotein subunit-9.68, polynucleotides encoding this polypeptide and DNA combination process to produce the polyupeptide.

本发明公开了一种多肽――黄素蛋白亚基-9.68，编码此多肽的多核苷酸和经DNA重组技术产生这种多肽的方法。

P28 紫色 Formation of a peptide bond 肽键形成–4侧链具有的反应–形成二硫键-其它反应 Tyr,His,Arg Review Isoelectric Point Titration滴定 of an amino acid 滴定 Gly幻灯片 18 His Lys Glu Reaction of amino acids Section 4 Protein isolation and purification 蛋白质分离及提纯 Working With Proteins Experimental techniques for protein analysis and characterization Purification steps A cell contains many types of proteins In the lab we want to isolate a single protein for experiments Purification steps We first grow cells or isolate tissues that contain the protein of interest We break open the cells to produce a crude extract Use centrifugation离心 to separate soluble from insoluble material We fractionate 分离 the protein mixture based on properties of such as size, charge affinity or solubility.

丹磺酰氯与氨基酸反应生成荧光性质强和稳定的磺胺衍生物，用于多肽链NH 用于多肽链 3末端氨基酸的标记烃基化反应(1) 2，4-二硝基氟苯2，4-dinitrofluorobenzene，二硝基氟苯(二硝基氟苯 DNFB也叫做试剂。DNFB在弱碱性溶液也叫做Sanger试剂试剂中与氨基酸发生取代反应，生成黄色化合物二硝基二硝基苯基氨基酸(dinitro phenyl amino acid， DNP氨基酸氨基酸)苯基氨基酸氨基酸(2)苯异硫氰酸酯(phenylisothiocyanate， PITC)在弱碱性条件下，与氨基酸反应在弱碱性条件下，生成苯乙内酰硫脲 PTH衍生物，(phenylthiohydantoin， PTH)衍生物，即PTH-氨基酸，此反应又称之Edman反应，该反应是蛋白质或多肽氨基酸序列测定常用的反应。

The invention discloses an antibody against cattle piron and method for preparation, wherein the antibody is obtained by using polypeptide selected from the following polypeptide family as the antigen immunological animal, said polypeptide family is formed by the following polypeptides,(1) polypeptide of the amino acid residue sequence 1 in the sequence table,(2) polypeptide obtained by removing 1-24 amino acid residues continuously from No.

本发明所提供的制备牛朊病毒抗体的方法，是以选自下述多肽家族之一的多肽为抗原免疫动物得到的抗体；所述多肽家族由以下多肽组成：1具有序列表中序列1的氨基酸残基序列的多肽；2从序列1的自氨基端第1位氨基酸残基开始向羧基端连续去除1至24个氨基酸残基，和/或从序列1的自氨基端第264位氨基酸残基开始向氨基端连续去除1至23个氨基酸残基得到的多肽。

Our company has more than ten years experience in produce the polypeptide products, technical capability is rich, there are three advanced production lines, the main products have collagen polypeptide , wheat polypeptide , soybean polypeptide , fish polypeptide , maize polypeptide ,etc., can use extensively in medicines, medical raw materials, health products , nutriment , function food , condensed food , food strengthener, young baby food and old food additive , sport beverage and food, etc.

我公司已有十多年生产多肽类产品的经验，技术力量雄厚，拥有三条先进的生产线，主要产品有胶原多肽、小麦多肽、大豆多肽、鱼胶原多肽、玉米多肽等，可广泛应用与药品、医药原料、保健品、营养品、功能食品、强化食品、食品强化剂、幼婴儿食品及老年食品添加剂、运动饮料及食品等。

According to the spetial conformational analysis and physical/chemical property analysis and by the aid of computer it can make molecular design of mimic polypeptide and designs the polypeptide molecule with activity for neutralizing LPS, and adopts the solid phase polypeptide chemical synthesis method to synthesize antibacterial/antiendotoxin mimic peptide with total or partial BPI bio-function.

本发明公开了通过计算机模拟设计、人工合成的66个抗菌／抗内毒素模拟肽，根据对BPIN端三个LOOP区的空间构象分析及物理化学性质分析，在计算机辅助下进行模拟多肽的分子设计，设计出具有中和LPS活性的多肽分子，用固相多肽化学合成方法合成抗菌／抗内毒素模拟肽，本发明的抗菌／抗内毒素模拟肽具有全部或部分BPI的生物功能，可以用于治疗G－细菌感染、人内毒素血症，具有分子量小、可以稳定重复生产的特点。

This study is design and preparation of active peptide analogues of mung bean trypsin inhibitor Lys fragment, Trichosanthes trypsin inhibitor I, carcinoembryonic antigen peptide and aspartame using solid and liquid phase peptide synthesis methods.

本论文研究活性多肽类似物的设计、制备和功能，分为四个部分，采用多肽固相和液相合成的方法，分别合成了活性多肽绿豆胰蛋白酶抑制剂 Lys片段长链16肽、天花粉胰蛋白酶抑制剂、癌胚抗原肽、甜味肽等一系列类似物，并测定了这些类似物的生物活性，有以下新的研究结果：1、furin酶抑制剂的设计、制备和功能；2、芳香氨基酸芳环之间的π-π共轭能部分补偿二硫键的作用；3、癌胚抗原肽Gly4被L-Pro替代可增加与HLA-A2分子的结合；4、肽键对甜味肽保持甜味是必须的。

Eight polypeptides were isolated from HBsAg and designated as p-1 through p-8 in the order of their decreasing mobilities and have molecular we...

本文报告HBsAg多肽的分离，各个多肽的分子量和它们的抗原性，并对一些主要多肽作为乙型肝炎疫苗的可能性进行讨论，认为HBsAg的多肽有可能作为乙型肝炎疫苗预防乙型肝炎病毒的感染。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。