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There are many reports about analysis of isozymes between male sterile lines and their maintainers, but different crops or varieties in same crop expressed dissimilarly. There were few reports about comparison study of isozymes between male sterile line in cabbage and its maintainer at present. Most reports about analysis of isozymes between male sterile lines and their mainainter were limited 1-2 isozymes.

有关细胞质雄性不育系及其保持系花药同工酶分析的报道已有相当多,但不同作物表现不一样,同一作物不同品种表现也有差异,而且目前有关甘蓝雄性不育系和保持系同工酶比较研究的报道极少,有关细胞质雄性不育系与保持系的同工酶分析的报道多限于1—2种酶。

In my experimentⅠcompare the purposes of different combinations of endoenzyme(MseⅠ-HindⅢ, MseⅠ-PstⅠ, MseⅠ-EcoRⅠ) and find that MseⅠ-HindⅢeasily finds thepolymorphism by primers combinations suiTab for AFLP analysis.And by means of this there aremany polymorphism strips. So the double endoenzyme of AFLP was exercisedfor the analysis of soybean cytoplasmic male sterility.In the cource of my experiment, 64 primerpairs of MseⅠand HindⅢwere exerted to analyse the twe mtDNA pools which are made ofsterilities and holdings and the polymorphisms of sterilities and holdings.

运用扩增片段长度多态性的方法,对独特的杂交大豆试材进行分析研究,通过比较MseⅠ-HindⅢ、MseⅠ-PstⅠ、MseⅠ-EcoRⅠ不同酶切组合的效果,结果表明MseⅠ-HindⅢ较容易找到多态性引物组合,而且具有较高的多态性,本试验选用MseⅠ-HindⅢ进行双酶切大豆线粒体DNA从而完成AFLP的分析工作,试验用64对MseⅠ和HindⅢ引物对两个不育系及保持系材料的线粒体DNA制成的pool进行AFLP标记分析,分析了不育系与保持系之间的多态性; 4。

The sterile line of Zhenshan 97A, Baoyuan A, g37A and Peiai64S had more Ca〓 precipitatesthan those in the fertile anthers since the pollen mother cell stage (which were mainly deposited in the epidermis, endothecium and vascular bundle).

野败不育系珍汕97A、包台型不育系包源A及温敏核不育水稻培矮64S和无花粉型不育系g37A的花药均从花粉母细胞时期开始出现较多的Ca〓沉淀,主要分布在药壁外层、药室内壁和药隔中。

Xinjiang Daye'is higher than male sterile lines. 2、The pistil characters of the male sterile plants BC1-2、BC1-19、BC1-21、BC1-22、BC1-23 are improved. Hairy cells of stigma of the male sterile plants enwrap loose mastoid cells. The area of mastoid cells exterior is larger. Hairy cells of stigma of the male sterile plants are longer and abduction.Their Style is larger and wide. The cubage of the epidermal cell is larger and the arrangement of passage cells is tight.

BC1-2、BC1-19、BC1-21、BC1-22、BC1-23的雌蕊的性状得到改良,改良的杂交后代不育株的柱头毛状体细胞将中心的乳突细胞包裹较疏松,乳突细胞明显突起且裸露面积较大,毛状体细胞较原不育系MS-4的相对长而外展且分泌物较多,;改良的杂交后代不育株的花柱较原不育系的花柱宽且长,表皮细胞的体积较大,通道细胞排列较紧密。

The dynamic changes in community height, coverage, abundance, above ground biomass and plant diversity in Stipa bungeana typical steppe was investigated by a quadrat method. There were six treatments: Unexclosure, 5-year old exclosure (5 a), 10-year old exclosure (10 a), 15-year old exclosure (15 a), 20-year old exclosure (20 a), and 25-year old exclosure (25 a) plots.

采用样方法对云雾山干草原区本氏针茅草地群落进行长期定位监测,对未封育样地和封育5,10,15,20和25年样地群落的平均高度、盖度、多度和地上生物量以及植物多样性进行实地调查分析,以研究围栏封育对草地植物群落特征动态变化的影响。

The study suggest:(1) The observation of satellites in root tip and multiplex PCR makers could accurately identify the homozygous and the heterozygosis of 1BL/1RS translocation.(2) Through the restoring degree we could confirm the success of the transfer. The combination of the restoring degree and the agronomy property could increase the efficiency of practical application.(3) This study proposed the transformation from the physiological male sterile to the genetic male sterile, and then establish a method of utilize the strong superiority crosses.

其中复合引物PCR更适合于回交后代中大量目标种子的筛选;(2)恢复基因转育后代定株与不育系测交,依据测交恢复度可准确确定恢复基因在回交后代的延续,恢复基因定株定向转育与性状选择结合可大大提高转育后代的实际应用效果;(3)本研究提出的粘类小麦雄性不育恢复基因Rfv1定向转育体系,可有效地实现小麦由化控强优势组合向三系强优势组合的定向转化,即生理型不育途径向遗传型不育途径的定向转化,进而建拓一套杂交小麦强优势组合多途径利用新体系。

As women prepare for pregnancy, it is important to acknowledge some of the more established causes of infertility.

对于准备怀孕的女性而言,更多的了解一些已经确定的不育的原因是重要的,它们包括

The main results were showed as follows:Through screen and identification of morphology in M_2 and M_3, respectively, the mutants with mutative traits of plant were found. In which, 11 mutations were leaf variations, including light-green leaf, deep-green leaf, durative yellowish leaf, temporary yellowish leaf, inlaid yellowish leaf, wrinkly leaf, smooth-edged curly leaf, splitting-shaped curly leaf, peduncular-shaped leaf, round leaf and larger leaf, 12 were mutations on plant type, including excessive branches, lacking branches, highness, dwarf-like, erect posture, reclinate posture, strong stalk, slender stalk, tufty branches, wrinkled plant, purple stalk and hair-covered plant, 14 were flower variations, including dense flowers, light-yellow petals, white petals, white-mosaic petals, wrinkled petales, back-rolled petals, narrow petals, larger petals, smaller petals, apetalous flowers, variable-numbered petals, fertile pistil protraction, sterile pistil protraction and multi-anther, and 5 were physiologic mutants, including genic male sterile, cytoplasmic male sterile, bud-dead, and early and later bloom.

主要结果如下:通过田间M_2筛选和M_3重复鉴定,在田间农艺性状方面共获得浅绿叶色、深绿叶色、转绿黄化叶、持续黄化叶、边缘黄化叶、皱缩叶、光叶型卷叶、裂叶型卷叶、薹叶形叶、宽圆叶和大叶等11种不同的叶部性状突变体;同时还筛选到多分枝、少分枝、高杆、矮杆、株型紧凑、株型松散、粗茎、细茎、丛生分枝、整株皱缩、紫茎、整株被毛等12种植株性状突变体,密花、浅色花瓣、白色花瓣、白斑花瓣、皱瓣、卷瓣、窄瓣、大瓣、小瓣、无瓣、花瓣数目不定、可育型柱头外露、不育型柱头外露和多雄蕊等14种花器性状突变体,以及细胞核雄性不育、细胞质雄性不育、死蕾、早花和迟花等5种生理性状突变体。

Compared with different type male-sterile line found at binucleate microspore stage non-1B/ 1R type male sterile lines and YS type male sterile lines both increase l7kD polypeptide compared with maintainers.

同时通过对比不同类型的不育系发现非1B/1R类K型不育系732A和YS光温敏不育小麦A3017在小穗发育的二核期均多1条分子量大小为17kD的多肽。

The results showed that the varieties and activity of POD isoenzyme expression exist in difference in anther of male sterility line and maintainer in different development stage in sweet pepper and hot pepper. POD isozyme activity of anther was very low in meiosis stage in male sterility line of sweet pepper. There is a POD special band in meiosis and pollen mature stage in male sterility line of pepper respectively.

结果表明不育系与保持系,在花药发育的不同时期POD同工酶表达种类及表达活性之间存在差异,其中甜椒不育系在减数分裂期POD同工酶的活性非常低,辣椒雄性不育系在减数分裂期及花粉成熟期分别多一条POD特征带。

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