多聚甲醛

1Cubic millimeter tissue sampling was fixed with the mix of 1% glutaraldehyde and 4% paraform in 4℃.

利用兔后腿软组织损伤、海水浸泡模型，分别于损伤后浸泡于海水中2小时，浸泡后分别进行各种低渗盐水冲洗，分别于干预处理后1h、3h、6h、8h、12h，在损伤周围0.5cm范围内的肌肉组织中取材，0.5cm3损伤组织用4%福尔马林固定，HE染色，光镜下观察损伤组织的细胞浸润情况，用免疫组化法检测受伤肌肉组织凋亡相关基因Caspase3表达情况；1mm3损伤组织用1%戊二醛一%多聚甲醛混合固定液(pH7.4 ) 4℃固定，电镜观察其细胞凋亡及细胞膜、线粒体等结构的变化；生化检测Na+，K+-ATPase活性，了解其组间的差别。

The paper introduced three processes to prepare isochroman from β-phenethyl alcohol and polyformaldehyde:HCl catalysis,AlCl_3 catalysis and toluene-p-sulfonic acid catalysis,and discussed its reaction mechanism,using toluene-p-sulfonic acid catalysis method to prepare isochroman ,the yield is 46%,purity is 96%.

用β-苯乙醇和多聚甲醛为原料，进行了HC l催化法，三氯化铝催化法和对甲苯磺酸催化法的合成路线的实验，并对其反应机理进行了探讨，应用对甲苯磺酸催化法合成异色满的合成路线，得率46%，纯度96%。

On-hoard determination of picoplankton is preferred, if possible. Otherwise, it is better to use paraformaldehyde or the mixture of paraformaldehyde and glutaraldehyde as fixatives, the samples stored in liquid nitrogen should be analyzed as soon as possible.

如有可能，应该尽量现场测定微微型浮游生物样品，否则建议采用多聚甲醛或其与戊二醛的混合物作为固定剂，将样品保存于液氮中，待返回实验室后尽快进行测定。

Th e tissues were immediately fixed by using one of the following fixatives for 24 hours before conventional tissue processing:(1)2.5%glutaraldehyde and 2%polyf ormaldehyde;(2)2.5%glutaraldehyde and 0.5%cetylpyridinum chloride to fix mainly the glycosaminoglycan;(3)2%OsO4 to fix the phospholipids (PH1);(4)4%OsO4 (PH2) and (5) 3%tannic acid.

取正常大鼠腹膜组织，分别以不同固定液固定：(1)2.5%戊二醛和2%多聚甲醛；(2)2.5%戊二醛和0.5%氯化十六烷基吡啶；(3)2%OsO4(PH1组)；(4)4%OsO4(PH2组)；(5)3%鞣酸。

SF-2 boundary lubricated bearings increase:Acid poly A with formaldehyde, with high wear resistance, bearing surface arrangement of the law must be fitted with a storage pit filled with grease, especially for high load low speed of rotation, rocking motion, and frequently borne, the opening and closing the difficult parts of the formation of lubrication in boundary lubrication conditions, long-term use without refueling and maintenance, where the fuel bearing life can be more extended, currently applicable to metallurgical machinery, mining machinery, hydraulic machinery, automobile parts, construction machinery, agricultural machinery.

SF-2加界润滑轴承：酸多聚甲醛一高耐磨性，轴承表面的法律安排，必须配备与油脂填补了蓄水坑尤其是高负荷低转速旋转，摇摆运动，并经常承担，开幕和闭幕的困难部分在边界润滑条件下，长期不加油和维修，那里的燃料轴承寿命可以更延长，目前适用于冶金机械，矿山机械，液压机械，汽车零部件，工程机械，农业机械使用润滑形成。

The retinae of 4 old cats (12 years,3-3.5 kg) and 4 young cats (1-3 years,2-2.5 kg) were selected.

取老年猫(12龄，3～ 3 5kg)和青年猫(1～ 3龄，2～ 2 5kg)各 4只的视网膜，经 4 %多聚甲醛处理后，用H 。

The retinae of 4 old cats(12 years,2.5-3 kg) and 4 young adult cats(1-3 years,2-2.5 kg) were collected.

取老年猫(12龄，2.5~3 kg)和青年猫(1~3龄，2~2.5 kg)各4只的视网膜，经4%多聚甲醛处理后用H。

B:Oocytes and early embryos were permeated in 0.5%Triton X-100 for 10 minutes,then fixed in 4% paraformaldehyde/PBS.Method C:Oocytes and early embryos were fixed in 1%PFA while permeated in 0.2%Triton X-100 at the same time for 1 hour.Method D: Oocytes and early embryos were fixed in precooled methanol(-20℃) for 15 min,then permeated in 0.5%Triton X-100 for 10 minutes.

方案A：先用4%多聚甲醛固定1h，再用0.5%Triton X-100穿透10min；方案B：先用0.5%Triton X-100穿透10min，再用4%PFA固定1h；方案C：用1%PFA+0.2%Triton X-100同时固定与穿透1h；方案D：用-20℃预冷甲醇固定15min，再用0.5%Triton X-100孵育10min。

,these animals were sacrificed via the way of infusing Zamboni fluid into aorta with the help of aortic cannlation.

方法云南成年健康雄性猕猴5只，平均体重6kg左右，氯氨酮（0.25ml／kg）肌肉注射麻醉后，Zamboni液经心脏主动脉插管灌注固定动物，取瓦脊髓节段入4％多聚甲醛后固定（4℃）6小时。

To investigate whether apoptosis is one of the mechanism for cell death in spiral ganglion cells and organ of Corti cells of guinea pigs'cochlea induced by ciSPLatin.

最后一次给药后24小时内处死动物、取材，分别多聚甲醛、戊二醛固定或快速液氮冻存，均取左侧耳蜗。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。