多细胞的

The progress of studies on Stylosanthes in the aspects of stylo anthracnose caused by the fungus Collectotrichum gloeosporioides and its genetic diversity and epidemiology, breeding, cytogenetics, cytobiology, tissue culture, protoplasm fusion, gene clone, genetic transformation, classification and molecular makers was reviewed in this paper.

摘要综述了柱花草的炭疽病危害、炭疽病原菌的遗传多态性及流行性、柱花草的抗炭疽育种、细胞遗传学、细胞生物学、柱花草组织培养、原生质融合、基因克隆、遗传转化、柱花草分类、分子标记、航天辐射育种等方面的研究进展。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

In order to overcome the problem of multidrug resistance in human epidemic carcinomata anti-adriamycin cells (KB-A-1), the antisense and antigene oligonucleotides were used to investigate their effectiveness on inhibiting the mdr1 gene expression. The effectiveness of antisense or antigene oligonucleotide on inhibiting the multidrug resistance was detected by MTT colometric assay and ELISA.

中文题名反义与反基因寡核苷酸及其萘二酰亚胺偶联物对靶基因表达的抑制作用研究副题名外文题名 The inhibition of the targeted gene expression by the antisense or antigene oligonucleotides and their naphthylimide-conjugated derivatives 论文作者李军生导师张元兴魏东芝教授学科专业生物化工研究领域\研究方向生物化学与分子生物学学位级别博士学位授予单位华东理工大学学位授予日期2001 论文页码总数106页关键词基因表达基因治疗反义寡核苷酸反基因寡核苷酸核酸馆藏号BSLW /2001 /Q78 /264 针对人表皮癌抗阿霉素细胞株（KB-A-1）的多药抗药性问题，本文从反义核酸和反基因核酸角度，通过MTT法检测细胞生长情况，ELISA法检测基因表达产物P-gp表达水平的变化，对寡核苷酸抑制肿瘤细胞MDR1基因表达的机制进行了探讨。

Results In dioestrous mice, many iNOS+ cells were found in the stroma of endometrium. There was no obvious differences between the immunized mice and the control mice. In the uterus on 3 day of pregnancy, however, there was obvious difference about the iNOS quantity in uterus between the immunized and the control mice. There was strong positive reaction on the epithelia of endometrium and the endometrial gland in the lamina propria in control mice, while the staining of iNOS in the uterus of SA-30 immunized mice was very weak, only a few of iNOS+ cells were observed in the myometrium.

结果 SA-30免疫后对间情期小鼠子宫内的iNOS没有明显影响，无论是免疫组还是对照组，在子宫内膜基质中均有大量阳性细胞分布，两者无明显差异；在妊娠第3d时，对照组的小鼠子宫中有较多iNOS表达，阳性标记主要集中在内膜上皮及固有膜中的子宫腺上，经SA-30免疫的小鼠子宫中，iNOS表达则显著减少，整个内膜标记极弱，只在子宫肌层内有少量iNOS阳性细胞分布。

Results In dioestrous mice, many iNOS+ cells were found in the stroma of endometrium. There was no obvious differences between the immunized mice and the control mice. In the uterus on 3 day of pregnancy, however, there was obvious difference about the iNOS quantity in uterus between the immunized and the control mice. There was strong positive reaction on the epithelia of endometrium and the endometrial gland in the lamina propria in control mice, while the staining of iNOS in the uterus of SA-30 immunized mice was very weak, only a few of iNOS+ cells were observed in the myometrium.

结果 SA-30免疫后对间情期小鼠子宫内的iNOS没有明显瘙痒影响，无论是免疫组还是对照组，在子宫内膜基质中均有大量阳性偏瘫细胞分布，两者无明显瘙痒差异；在妊娠第3d时，对照组的小鼠子宫中有较多iNOS表达，阳性标记主要集中在内膜上皮及固有膜中的子宫腺上，经SA-30免疫的小鼠子宫中，iNOS表达则显著减少，整个内膜标记极弱，只在子宫肌层内有少量iNOS阳性偏瘫细胞分布。

In cross-sectioned root form 2 middle bark layer next Internal bark layer especial structure of regularly array; epidermis cell of stems and leaves have developed thickened epiderm, leaves epidermis covers hair, it formed developed vascular bundles.(4)Chromosomes of Agropyron cristatum is tetraploid .

根横切面上形成了在紧靠内皮层的两层中皮层细胞有规则整齐排列成两环的特殊结构；茎和叶表皮细胞均具有发达的角质层，叶表皮多密被毛，其形成了发达的维管束及维管束鞘。

In the biological functionaddition, both biochanin A and hesperitin might inhibited the colony -formation forming ability of K562 cells through up-regulated Notch signaling. Besides,The treatment with biochanin A and hesperitin enhanced the erythroid-differentiation of K562 cells. It is important to investigate Tthe molecular mechanisms of biochanin A and hesperitin to regulate the endogenous CBF1-dependent Notch signaling will be further investigated.

在生物功能方面，biochanin A与hesperitin可能经由Notch讯息传导的活化，抑制了K562细胞群落的形成，除此之外，这两种药物均明显促进K562细胞往红血球分化。biochanin A与hesperitin对於CBF1所媒介之内生性Notch讯息传导的分子调控机制仍有许多问题尚未厘清，需要更多的实验与研究来讨论。

Pylori infection can result in hypochlorhydria,the molecular mechanisms underlying this phenomenon remain poorly understood.Most of formerly study focused on the immunoregulatory cells.

以往的研究多集中在免疫调节细胞，我们的研究结果表明，VacA毒素能够直接诱导胃壁细胞顶膜通透性的增加，导致胃酸分泌的减少。

Bone marrow mesenchyma stem cells are stem cells that occupy self-renewal and multiple differentiations into cells, which have been recognized as the optimal origin for liver cell.

骨髓间充质干细胞既具有自我更新的能力，也具有在合适的微环境里向多系横向分化的潜能，在体外可以被诱导分化为肝细胞，理论上可提供丰富的肝细胞来源。

A large multinucleate cell found in growing bone that resorbs bony tissue,as in the formation of canals and cavities.

破骨细胞一种巨大的多核细胞，发现于吸收骨组织的生长骨中，如在管和腔的形成时的生长骨中

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。