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The results showed that precursor feeding (phenylanine, sodium acetate and benzamide) and mixed sugar as production medium resulted in significant increase of taxol production not only in the one-liquid-phase cultures of Taxus chinensis var. mairei but also in the two-liquid-phase cultures. And the integrated function of precursor feeding and mixed sugar in the two-liquid-phase cultures gave more significant increase of taxol production significant increase of taxol production, which was four times more than that of the control (the one-liquid-phase culture without precursors and mixed sugar).

结果表明前体饲喂(苯丙氨酸,醋酸钠和苯甲酰胺)和蔗糖与麦芽糖配伍作为生产培养基糖源不仅对红豆杉细胞悬浮培养,而且对其两液相培养的紫杉醇产量提高均有明显的促进作用,与对照组相比,在南方红豆杉细胞两液相培养中,采用前体饲喂和蔗糖与麦芽糖配伍作为生产培养基糖源,紫杉醇产量提高4倍多。

Methods: The olfactory nerve layer and glomerular layer was isolated from the olfactory bulb of three-day old rats, Enzyme digestion method was used to isolate cells and the cells were purified by differential adhesion method. Freshly isolated OECs were plated onto poly-L-lysine-coated tissue culture and then incubated for 2 days. The purity of OECs was identified by immunocytochemistry of NGFRp75 and S100 and Hoechst33342 counterstain method.

从新生SD大鼠(3d)嗅球中迅速分离嗅神经层和嗅颗粒层,采用酶消化法分离细胞,差速贴壁法纯化细胞,接种于多聚赖氨酸包被的培养板内培养2d,采用NGFR p75和S100蛋白双标免疫组化、以Hoechst33342复染鉴定OECs的纯度。

Two cases were isointense or hypointense signal on T1WI and heterogeneous hyperintense signal on T2WI and T2WI STIR. Conclusion Enhanced CT clearly showed calcification and blood supply of the tumor while MRI showed small hemorrhage, cystis foci, necrosis and central stellate scar of the tumor. MRI can provide more useful information in detecting chromophobe cell renal carcinoma.

结论肾脏嫌色细胞癌属少血供肿瘤,CT能清晰显示肿瘤内钙化灶和血供情况,而MRI容易检出肿瘤内小出血灶、囊变坏死灶以及中央星状瘢痕,可对肾脏嫌色细胞癌的诊断提供更多更有价值的信息。

There are 12 cases were death. ConclusionsPersisting hypoxemia always results in hyperpotassemia, cellular acidosis, increasing of blood consistence, heart overexertion as well as hepatocyte edema,degeneration even cytoclasis and so on.

死亡12例。结论持续存在的低氧血症可导致高血钾,细胞内酸中毒,血液粘稠度增加,心脏负荷增加,肝细胞水肿、变性甚至坏死等,严重的可以出现多脏器功能衰竭、DIC发生甚至死亡。

GTW-containing serum sign ificantly suppr essed pro liferation of spleno cytes . serum of GTW fed for more day s suppressed

细胞增殖反应有明显的抑制作用,且IC50分别为0.26μg/ml 和0.16μg/ml,雷公藤多苷含药血清对细胞增殖反应也有明显的抑

SIgA cell distributed in interstitice Harderian Gland, mainly in epithelium and around gland epithelium of the duct and acini, especially mature plasma cell clung to gland epithelium.

气管中SIgA细胞主要分布在气管黏膜上皮之间和固有膜处,局部黏膜上皮形成小隐窝,隐窝内和隐窝下的固有膜中有较多的SIgA型浆细胞。

In present study, we demonstrated that μ opioid receptor and D1 dopamine receptor could form heteromeric complexes in membranes preparation from HEK293T cells transiently cotransfected with both receptors and from mice striatum.

在本工作中,我们通过免疫共沉淀和BRET法证明,HEK293T细胞上瞬时表达的μ阿片受体和D1多巴胺受体能形成异源二聚体,并证实μ阿片受体和D1多巴胺受体异源二聚体存在于小鼠纹状体中。

The confocal laser scanning microscopy revealed that the cyto-skeleton of MEL and MEL-M organized incompactly while the F-Actin organization of MEL-W was compact.

由激光共聚焦显微镜观察到,MEL和转突变型p53的MEL细胞骨架排列松散,而转野生型p53的MEL细胞骨架交联多且致密。

MAIN OUTCOME MEASURES: Morphologic changes of differentiated ventral mesencephalic precursors; Expression of DN-related common marker genes before and after the induced differentiation.

主要观察指标:①多巴胺能前体细胞诱导分化后形态变化。②常见多巴胺能神经元标记基因在诱导分化前后过程中的表达。

Methods: 1. A model of Transwell has been developed for the co-culture nearly pure microglia cells and dopaminergic neurons.

研究方法 1、用原代培养的方法建立小胶质细胞与中脑多巴胺能神经元体外培养的Transwell细胞模型。

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推荐网络例句

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。