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The brain tissue of the different fetal age rat could be cultured into neural stem cells with the most number of cells for 12.5 dags embryo, which furthermore differentiated into neurons and glial cells.

胎龄为12.5天胎鼠大脑皮质培养出的神经干细胞数量最多,可分化为神经元、神经胶质细胞及少突胶质细胞。

In the hippocampal formation there were many terminals containing AchE and they distribute mainly in the deep part of stratum oriens and both sides of granular layer in the area dentata.

AchE组化染色显示海马和齿状回内有大量AchE阳性终末,主要位于多形细胞层的深部和齿状回颗粒细胞层两侧。

In eleven months at the most, every one of those weak and devitalized cells, every one of those bent and crippled bones, will be replaced by new, strong, vigorous tissue.

最多花11个月的时间,每一个虚弱和衰老的细胞、每一个致使你残疾的细胞都会被新的、强壮的、充满活力的细胞所取代。

Objective To investigate the functional mechanism of Chinese drug disodium cantharidinate reversing multidrug resistance of tumor cells.

目的 观察斑蝥酸钠对多药耐药的白血病细胞(K562/AO2)细胞是否有逆转作用,并初步探讨其逆转机制。

The cell culture taken from a lens of mild cataractous degree was characterized by a majority of smaller epitheloid cells showing a homogeneous cell picture and a distinct and relatively large nucleus.

结果显示:轻微程度白内障水晶体上皮细胞在培养时多为较小而形状均匀的细胞,其细胞核明显而较大。

Recently, a new pluripotent stem cell establishing method was reported that, mouse and human differentiated cells could be induced reprogrammed into a pluripotent state by expressing exogenetic stem factors such as Oct4, Sox2, et al, through retroviral transduction.

通过反转录病毒载体系统,在小鼠和人类高度分化细胞中表达干细胞因子Oct4, Sox2, Klf4和/或c-Myc等基因,再经过干细胞标志因子Nanog或Oct4筛选,可以获得与ES细胞特性十分近似的诱导多能干细胞系。

Methods: A model to evaluate lymphocyte proliferation stimulated with a polyclonal activator, concanavalin A, was established by vital dye carboxyl fluorescin diacetate succinmidyl ester labeling technique. Effects of the different doses of anisomycin on the lymphocyte proliferation were estimated by flow cytometry and MTT methods. The propidium iodide labeling technique was applied to assay the effect of the different doses of anisomycin on changes of the lymphocyte cell-cycle stimulated by ConA or by phorbol ester plus Ionomycin. The percentage of the expression level of CD69 and CD25 on the activated lymphocytes was evaluated by fluorescin-conjugated monoclonal antibody double labeling technique.

以活体染料羧基荧光素乙酰乙酸琥珀酰亚胺酯染色,建立在多克隆刺激剂刀豆蛋白A刺激下评价小鼠淋巴细胞增殖的模型,通过流式细胞术和MTT法分析茴香霉素在不同剂量下对淋巴细胞增殖的作用;采用碘化丙锭染色分析茴香霉素对ConA或佛波醇酯加离子霉素刺激的小鼠淋巴细胞周期变化的作用;利用荧光标记的单克隆抗体双染技术和流式细胞术观察茴香霉素对小鼠CD3~+T细胞早期及中期活化标志分子CD69和CD25表达的影响。

Fig.3 The astrocytes in the area of gliosis around the anaplastic astrocytoma displayed Fas expression.

在多形性胶质母细胞瘤中内增生的血管内皮旦Fas强阳性,瘤细胞变为Fas阳性

Methods Both immatured and matured oocytes were stained by Mito Tracker Green FM.

方法未成熟卵母细胞和成熟卵母细胞均用Mito Tracker Green FM染色,经多聚甲醛固定后在激光扫描共焦显微镜下观察线粒体的分布。

MATERIALS AND METHODS: hMSCs labeled with enhanced green fluorescent protein were transplanted into the lateral ventricle of neonatal mouse brain. At 0, 9 and 14 days post-transplantation, MICE were sacrificed and their brains were fixed with 4% paraformaldehyde. The engraftment of transplanted cells in coronal section of the recipient mouse brain was examined wider a fluorescent microscope. Indirected immunofluorescent staining was used to detect the expression of neural proteins of these grafted cells.

材料与方法:将标记绿色荧光蛋白的人骨髓间充质于细胞植入到新生3d的小鼠侧脑室中,分别于移植后0d,9d和14d处死受休鼠,取其脑组织,4%多聚甲醛固定后行冠状面冰冻切片,荧光显微镜下观察hMSCs的植入,激光共聚焦显微镜检测植入细胞神经特异性蛋白的表达,定位双重标记的植入细胞。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。