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AIM: To determine subpopulations and cytokine expression of nave and memory T cells by polychromatic flow cytometry.

目的: 利用多色流式检测技术探讨初始和记忆T细胞亚群与细胞因子表达之间的关系。

As an ideal prime immunization vaccine,the DNA vaccine has the advantages of high purity,continuous expression of antigens with low dose, repeatable immunization and inducement of memory CTL with high titer.Similarly, recombinant virus can not only highly express the target antigen,simulate the inducement process of virus infection on body immunity,and induce various cytokines,but also be beneficial to the interaction of high-affinity antibodies with T cell receptors,and the generation of Th1 reaction and CTL reaction.

DNA疫苗抗原纯度高,持续表达低剂量的抗原,可多次重复免疫以及诱导高效价记忆性细胞毒性T淋巴细胞反应,是理想的用于初次免疫的疫苗;重组病毒能高效表达目的抗原,模拟病毒感染诱导机体免疫的天然过程,能诱发多种细胞因子,而且有利于高亲和性抗体和T细胞受体的选择,可以有效地产生Th1型和CTL反应。

More prophase and prometaphase cells may be obtained by this technique.

在培养基中加入5-溴脱氧尿嘧啶核苷使细胞同步化6—8小时,可以得到较多的前期和前中期分裂细胞。

Eventually we might be able to take T regs out of cord blood in order to have a source from which to grow more.

最终,我们可能会把T调节细胞从脐带血中提取出来作为培养源以便生成更多的T调节细胞。

Rbkinesin-6 ablated cells could not finish the telophase/cytokinesis phase, and became binuclear/multinuclear cells.

Rbkinesin-6缺失的细胞无法完成有丝分裂末期/胞质分裂,进而转变为双/多核细胞。

To investigate the effects of Cytochrome P450 17α-hydroxylase/17, 20-lyase (CYP17) small interfering RNA on the expression of CYP17 and androgen biosynthesis in theca cells from women with polycystic ovarian syndrome.

探讨细胞色素P450 17α-羟化酶/17,20裂解酶(CYP17)小干扰RNA对CYP17基因表达和多囊卵巢综合征卵泡膜细胞雄激素合成的抑制效果。

Experimentally, multi-pores are observed on leukocyte lysed with 3D electrodes and just single pore with planar electrodes. This is believed to be due to more uniform electric field of 3D electrodes than that of planar electrodes. Intracellular elements are extracted through the membrane as observed by increased intensity of Trypan blue when diluted by saline solution. We also demonstrate the lysing percentages at same flow velocity to explain the performance of 3D electrode is superior to that of planar electrode.

在实验方面,可发现细胞在3D 电极的形况下,是有多个破孔出现,有别於在平面电极的单个破洞,也发现当细胞出现破孔时,经由Trypan blue的辅助观察以及在食盐水稀释情况下,细胞内液是往外流出的,另外由裂解比率的结果,3D电极也是优於平面电极。

Recent study have found that statins have lipid-lowering effect, multi-effect, including antioxygen, anti-inflammatory, immunomodulatory, lower levels of cytokines, improve endothelial cell function and prevention of thrombosis, reduce inflammation reactions.

近来的研究发现他汀类药物除了降脂作用外,还具有多效性,包括抗氧化,抗炎,免疫调节,降低细胞因子水平,提高内皮细胞功能和防止血栓形成,降低炎性反应等。

An accurate immunophenotyping of leukemia ca n be realised when leukemic cell colony is displayed and analyzed by CD45/SCC p attern and gating strategy, which is important for clinical treatment and progno sis judgment.

利用CD45 Percp/SSC设门技术及流式细胞仪多参数分析技术,可对白血病细胞群体进行准确的免疫表型检测和研究,有助于指导临床治疗及判断预后

More than 95% purity achieved after removal erythrocyte, nonparenchymal cells and dead cells with multi-step low-speed centrifugalization.

通过多步低速离心可去除红细胞、其他非实质细胞和死亡的肝细胞,其纯度可达95%以上。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。