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Then transplantation of the sciatic nerve from donor rats to the recipient rats in two groups were progressed.7 days after nerve transplantation,the contents of cd4 and cd8 in peripheral plasma of recipient rats were detected by flow cytometry.8 weeks after nerve transplantation,transplanted nerve were observed under light microscope.results:compared with nonirradiated group,the percentage of cd4 and the ratio of cd4 to cd8 in uvb irradiated group were significantly decreased(p<0.05,p<0.01),cd8 percentage was significantly increased(p<0.01).conclusion:uvb-irradiated donor splenocytes were injected in the recipient rats through caudal veil,which could induce immunological tolerance to allogeneic transplantation.

未照射组注射未经紫外线照射的供体脾细胞,照射组注射经紫外线照射的脾细胞,7 d后移植供体神经。术后7 d行外周血t淋巴细胞亚群检查,术后8 w行移植神经组织学检查。结果:照射组cd4+百分率、cd4+/cd8+比值较非照射组明显降低(p.01,p.05),cd8+百分率较非照射组明显升高(p.01)。组织学检查显示,照射组的再生神经较非照射组多。结论:尾静脉内注射经紫外线照射的异基因脾细胞可诱导对异体神经移植的特异性免疫耐受。紫外线;脾细胞;免疫耐受;坐骨神经

DATA EXTRACTION: Totally 122 literatures about the cellular oncosis were collected, and 26 of them were reviews, There were 26 literatures concerned to the cardiovarcular disease; other literatures studied the detection and identification of cellular oncosis in non-cardiovascular system.

资料提炼:共收集到122篇关于细胞胀亡的研究文章,其中26篇为综述类文章,与心血管疾病关系密切的文献也有26篇,其他文献主要体现了细胞胀亡在心血管外的系统的发现和鉴别,并且文献中区分细胞胀亡和细胞凋亡的方法多重复。

objective:to analyze the difference between mammary duct ectasiaand plasma cell mammitis.methods:the data of 24 cases of mde and 28 cases of pcm,including clinical manifestations,distinguished diagnoses,surgical therapy,pathological results,were analyzed.results:the clinical manifestations of mde are nipple discharge and breast mass,which should be distinguished with breast mass and early stage of breast cancer.the treatment is local resection.major pathological changes are duct ectasia and obvious periductal inflammatory changes.the clinical manifestations of pcm are breast mass and inflammatory changes,which should to be distinguished with advanced breast cancer and inflammatory breast cancer.the surgical treatment is extensive resection.the major pathological changes are inflammatory reaction and multiple abscesses in breast tissue.conclusion:mde and pcm are significant different,and they are different progressing stage of disease,thus the two diagnoses are independent.

目的:分析乳腺导管扩张症和浆细胞性乳腺炎临床上的差异。提出各自独立诊断的论据。方法:结合24例乳腺导管扩张症和28例浆细胞性乳腺炎对两病的临床症状、鉴别诊断、手术治疗、病理结果进行比较分析。结果:乳腺导管扩张症临床表现为乳头溢液和乳腺肿物,主要与乳腺肿物和早期乳腺癌鉴别诊断,手术以局部切除多见,病理表现为导管扩张及导管周围明显炎性改变。浆细胞性乳腺炎临床表现为乳腺肿物和炎性改变,主要与晚期乳腺癌和炎性乳腺癌鉴别诊断,手术切除的范围较大,病理变化以乳腺组织的炎性反应和多发性脓肿为主。结论:乳腺导管扩张症和浆细胞性乳腺炎有明显的差异,应作为这两种疾病独立诊断。

Results: Of all the semen samples, 8 were HCMV positive, 4 HSV-Ⅱ positive, but none were both HCMV and HSV-Ⅱ positive. HCMV late antigens were positively and HCMV early antigens negatively expressed in the spermatogenic cells of the 8 HCMV positive cases. In the 4 HSV-Ⅱ positive cases, 3 were positively and 1 weakly positively expressed. In the semen of the 12 positive cases were found large numbers of immature spermatogenic cells, with different manifestations of apoptosis, such as chromatin pycnosis, vacuoles, damaged nuclear membrane, and apoptotic bodies, but without virus infection-induced specific morphological alteration.

结果:83例精液样本PCR检测HCMV阳性8例,HSV-Ⅱ阳性4例,未发现2种病毒同时阳性病例。8例HCMV阳性病例ICC标记生精细胞HCMV晚期抗原均见阳性表达,HCMV早期抗原均为阴性表达;4例HSV-Ⅱ阳性病例ICC标记生精细胞3例阳性表达,1例弱阳性。12例病毒阳性病例均发现较多未成熟生精细胞,并有不同程度的核染色质固缩、出现空泡、核膜破损、核崩解、凋亡小体等凋亡现象,但未能找到病毒感染的特异性形态学改变。

Ornithine decarboxylase, a rate-determining enzyme of the polyamine biosynthetic pathway, is involved in the control of cell cycle, differentiation, cell growth and apoptosis. Previous studies described that protein kinase Cd regulates ODC activity. In this thesis, human leukemia cell line, HL60 cell, was used for the treatment with a specific PKCd inhibitor, rottlerin, to measure the effects on cell growth.

鸟胺酸去羧化(ornithine decarboxylase,ODC)为多胺酸起始与决定反应速率的酵素,对细胞周期、细胞生长、分化及细胞凋亡扮演一重要角色;在寻找肿瘤治疗及预防药物研究中,纯化植物天然物质是其中来源之一,研究者筛选药物时,测定对鸟胺酸去羧化活性是否具抑制效果是一个重要的试验。

Animals, as material, were used to study the toxicity of outer substance and made sure the estrogen effect of BBP by whole animal experiment, and the chronic toxicity of BBP was studied, designed and valued through several dosages and different exposed time. The result showed that BBP could make testis, epididymis and prostate withdrawl. Tectology changes were observed in testis, epididymis and liver. Especially the atrophy and degeneration of seminiferous tubules, decrease of spermatogenic cell and the turbulence of Sertoli cell and spermatogenic cell of seminiferous epithelium were also observed. The linkage between cells vanished.

明确了BBP的雌激素效应,并分别用多剂量和不同染毒时间来研究、设计、评价BBP在体内的慢性毒性,结果表明:BBP能引起雄鼠睾丸、前列腺、附睾性腺的萎缩和肝脏肿大,改变睾丸、前列腺、肝脏的组织形态学,尤其引起睾丸曲细精管萎缩、变性,各级生精细胞减少,生精上皮内生精细胞和Sertoli细胞结构明显紊乱,细胞间连接结构消失等。

To explain relationship between the effect of CLD on senile Demention and neuron in hippocampal area. The change of natural aging mice's neuron in hippocampal area were studied by applying microscope and transilluminating electric microscope. Results: Comparing with the young control, the old control mice's hippocampus had less small pyramid cells, the nerve cells denaturalizing and their branches reducing in the microscope. Hippocampal nerve cells were degenemiceing, with nucleus membrance crinkling like wave shape were not clear, and chromatin condensing with high electron density, heterochromatin accruing, part cell organs inclosing nucleus, nucleus condensing in the EM.

为了阐明菖龙丹抗老年性痴呆作用与海马区神经元结构的关系,运用光镜和透射电镜观察了菖龙丹对自然衰老小鼠海马区形态的影响,结果显示老年对照组小鼠光镜下海马锥体细胞较青年对照组数量减少,细胞体积变小,神经元变性,分枝减少;电镜下海马神经细胞发生退化改变,核膜皱缩,呈波浪状,膜结构不清晰,核染色质浓缩,电子密度高,异染色质较多,部分胞浆细胞器向细胞核聚集,有的可见细胞核固缩。

Methods A routine culture system of melanocytes was established, cells were irradiated with different dosages of UVB(30 and 90mJ/cm2 respectively), and then incubated with different concentrations of TMP for 72h. MTT method, NaOH assay and DOPA oxidization reaction were employed to determine the cell proliferation, melanin synthesis, tyrosinase activity of melanocytes.

常规分离培养正常人黑素细胞,分别用30mJ/cm2、90mJ/cm2的UVB照射细胞,然后加入100μg/ml、300μg/ml、600μg/ml的TMP孵育至72h,采用噻唑蓝法测定细胞增殖情况;多巴氧化法测定酪氨酸酶活性;NaOH裂解法测定黑素含量。

After culture on coverslips and bovine cortical bone slices for 1, 3, 5, and 7 days, morphologic features, TRAP staining and bone absorptive lacunae were observed to evaluate osteoclast cells.

结果显示,当培养细胞在RANKL和M-CSF联合作用下,TRAP阳性染色的单核和多核破骨细胞可在3天内迅速形成,骨片吸收实验显示骨片上出现典型的骨吸收征象;在M-CSF(50ng/ml)协同作用下,RANKL呈剂量依赖型诱导TRAP阳性破骨细胞生成。

The numbers of ANAE(superscript +) T cells in the spleen and head kidney were more than those of thymus, therefore, it is suggested that the matured T cells are emigrated thymus to spleen and head kidney.

实验结果进一步证明了胸腺中的T细胞多为不成熟的T细胞,此外,还含有大量的IgM细胞;而脾脏、头肾内的ANAE T细胞多于胸腺,推测胸腺成熟的T淋巴细胞迁移到头肾和脾脏。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。