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The 3-color cytometry can be successfully used to characterize the subsets of immune cells in decidua and periphery blood.

早孕妇女蜕膜中具有与外周血不同的免疫细胞组成,应用多色荧光标记的流式细胞术能够简单、直接地鉴定各免疫细胞亚群。

We observed that a majority of dsRNA was localized in the nuclear periphery discontinuously after 24 hours of transfection. Time course showed that the labeled dsRNAs were maintained in cultured silkworm cells for up to 5 days. After six days, the signal was too weak to be detected.

用荧光显微镜观察FAM标记的dsRNA在细胞内的定位,发现在转染24小时后,dsRNA开始越来越多的聚集在细胞核的周围,并呈不连续分布,细胞内的荧光强度也越来越强。dsRNA在家蚕细胞中可以维持5天的时间,但到第6天时细胞内已检测不出荧光信号。

ABSTRACT: Objective To investigate the effects of epigallocatchin gallate on telomerase activity of bladder carcinoma cell line T24 and BIU87 and its possible mechanisms.

目的 探讨茶多酚对膀胱肿瘤细胞株T24和BIU87的端粒酶活性影响及其可能机制。方法用MTT法测定治疗组、阳性对照组、阴性对照组的细胞抑制率,用流式细胞仪测定细胞凋亡率。

It is suggested that the fetal liver cells injected into the bone marrow cavity can settle down and proliferate here and the volume of injection should be limited.

这一研究结果说明,直接注入骨髓腔内的胎肝细胞,能在骨髓腔内定居、增殖,实验结果还提示,用骨髓内注射法进行造血细胞移植时,注入骨髓腔内的细胞悬液量不宜太多。

Methods After fixed by paraformaldehyde, vascular endothelial cells from pigs were co-cultured in vitro with natural killer cells from Chinese monkeys at different ratios. The change of the cytotoxicity of natural killer cells occurring after this contact and the content of IFN-γ and TNF-α in the supernatants were detected.

将中国猕猴的NK细胞与多聚甲醛固定后的猪血管内皮细胞按不同比例进行体外混合培养,检测NK细胞在以不同比例接触靶细胞后的杀伤活性变化以及培养液上清液中的IFN-γ及TNF-α含量。

In other areas, sheets of epithelioid cells with prominent nuclear pleomorphism were seen; scattered tumor giant cells and multinucleated tumor cells were also present (Figure 1, B ).

在其他区域,可以见到成片的具有明显多型现象的上皮样细胞;散在的瘤巨细胞和多核瘤细胞也可以见到(图1B)。

After 24 hours of incubation,the cells became bigger,irregular and typical DCs with pseudopodium. The floating cells were separately induced to CIK cells and LAK cells. CIK cells proliferated rapidly and became a lot of large growth-colonies.

悬浮的细胞分别用于诱导生成CIK和LAK细胞,3天后CIK细胞生长迅速,出现大量的生长集落,体积明显变大呈多形性。

The results showed that SiO2 and total protein contents, PAM numbers and LDH activity in silicotic rats decreased gradually with the severity of silicotic pathologic changes.

结果表明,矽肺大鼠BALF中SiO2粉尘和总蛋白量以及细胞总数和LDH活性,均随矽肺病变加重而逐渐减少。虽然,BAL对不同程度矽肺病变均能洗出一定量的细胞和非细胞成分的物质,但以早期病变组排出量最多。

In this report, immunohistochemistry and in situ hybridization techniques were perfected to examine the expression of bFGF, fibroblast growth factor receptor-I and bFGFmRNA of cultured HEP-2 laryngo-tumor cell and 803 gastro-tumor cell and of the solid tumor tissue formed by implanting the cultured cells into subcutis of nude mice, intending to elucidate the correlation between the expression level and the tumor formation.

本研究建立了免疫组化和原位杂交的检测方法,通过对体外培养的HEP-2喉癌细胞和803胃癌细胞和这两株细胞在裸鼠皮下形成的实体瘤组织中bFGF,纤维细胞生长因子受体-1(fibroblast growth factor receptor-1,FGFR-1)和bFGFmRNA的检测试图揭示三者在体外和体内表达以及与肿瘤形成的关系,从而为这方面的研究提供更多的材料。

Japonicum, covering 90% of its total genes; find 8400 protein-coding genes; set up the largest functional gene database and clone libraries in the world; for the first time, identify as many as 3260 proteins from variety of life stages of S. japonicum; analyse the eggshell proteins and tegument proteins, which were the putative targets for drugs and candidates of vaccines; reveal a large quantity of genetic polymorphisms in the genes of S.

对人CD34+造血干/祖细胞进行了转录组和蛋白质组学研究,鉴定了370个蛋白质,这是迄今为止鉴定最多的CD34+细胞蛋白,并且鉴定了各种转录组研究没能发现的133个CD34+细胞蛋白,发现了CD34+细胞具有可塑性的新的证据,证明了反义核酸未能完全阻止其相应基因的表达,并提出一些蛋白的异质性是由于翻译后修饰造成;2。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。