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We also confirmed in this paper that it is injected somatic cells but not broken cytoplasm formed multi blastomere through mitosis in the reconstructed embryos.

在本实验中还通过用DNA染料(Hoechst-33342)对核移植体进行染色的方法,对融合后成纤维细胞在核移植体内不同时间的运行及发育模式进行了观察,确认了是成纤维细胞经有丝分裂形成多个卵裂球而不是由于细胞质碎裂形成的两个或多个碎块。

The results showed that pendimethalin is a herbicide interfering cell division,The mito-sis of pendimethalin treated root was arrested at metaphase with contracted and condensed chromo-somes and produced a series of abnormal,polymorphic cells such as multinucleate,multinucleolus,micronucleus and polyploid cells.

结果表明,除草通是一种影响细胞有丝分裂的除草剂,其作用方式在于阻止分生组织细胞从前期向中、后、末期的过渡,引起染色体凝集、短缩以及多核、多核仁等一系列畸形与异常变化。

Gradually, bulky cells with filamentous structures predominated the cell population and multinucleated giant cells accumulated.

逐渐地,细胞群中多呈含有丝状细胞质构造的肿胀细胞且可见到多核巨细胞的出现。

The results of observation on the microstructure of Karelinia caspica, Tamarix stromongolica, Salsola collina. Cheno Dodium glaucum, stipa bangenana show that the nutritional organs of these halophytes present some adaptability structures as follows:(1) sunken stomas, thick cuticle and orderly arrangement of epidermal cell;(2) equilateral leaves with developed palisade tissue, or squama-shaped leaf;(3) well-developed water-storing tissue in stems and leaves;(4) crystal cells, salt glands in most of the leaves and assimilating branches.

通过对花花柴、甘蒙怪柳、猪毛菜、灰绿藜、长芒草五种盐生植物显微结构观察,这五种盐生植物营养器官的形态结构均呈现一定的适应性特征:(1)气孔器下陷,角质层加厚,表皮细胞排列紧密而整齐;(2)多为等面叶,叶片栅栏组织发达,或退化为鳞片状,而具同化枝;(3)茎、叶多为肉质化,贮水组织发达;(4)具有含晶细胞,枝叶具盐腺。

Its main features are: algae body as a single cell or even into a variety of groups; cell walls composed of silica and pectin, but also by the structure of the shell and the shell under the combined set of the Cheng, vertical section view was "soap-box"-shaped; in the upper and lower shell surface of the shell are patterns arranged in patterns on both sides of the main mode of symmetry and radial symmetry is divided into two categories, for the classification of the most important basis; be able to campaign Type in the shell has a shell side seam, can not be changed no shell seam types; pigment body sheet or ribbon, 1 or 2, or for multiple small discoid, yellow green or brown, containing chlorophyll a, c, also contains fucoxanthin and silicon A flavin and other photosynthetic pigments; storage material mainly for oil droplets; reproduction mainly for cell division and cell division in diatoms obvious features are: each cell division, resulting from two sub-cells,, only one and the mother cell and so big, the other one is smaller.

其主要特征是:藻体为单细胞或连成各式群体;细胞壁由硅质和果胶质组成,而且在结构上都是由上壳和下壳套合而成,纵断面观呈&肥皂盒&形;在上下壳的壳面上有花纹,花纹排列的方式主要分为两侧对称和辐射对称两大类,为分类上的最重要依据;能运动的种类在壳面都有壳缝,不能动的种类均无壳缝;色素体片状或带状,1个或2个,或为多个小盘状,黄绿色或黄褐色,含叶绿素a、c,还含有墨角藻黄素和硅甲黄素等光合色素;贮藏物质主要为油滴;繁殖方式主要为细胞分裂,硅藻的细胞分裂的明显特点是:每次细胞分裂所产生的2个子细胞中,仅有1个和母细胞等大,另1个则稍小。

At present time people has found that various oxidants exist in injured cells, including peroxide and other free radical (eg: peroxide anion and hydroxide radical ion); these ROS also exist in abnormal cells arose from cell cleavage block and cell function loss.

目前已经发现,损伤的细胞中含有较多的各类氧化物,包括过氧化氢以及其它自由基(例如过氧化物阴离子和氢氧根离子);在因细胞分裂阻断以及细胞功能丧失所引起的异常细胞中也含有这些活性氧类物质。

Biochemical indicator such as TG and SF were unregulated while 1 system or 2 system reduced in peripheral blood; Bone marrow hyperplasia showed active or active obviously. Complete morphological nucleated cells mature erythrocytes or platelet hemophagocytic cells were found in 100% marrow slides. Marrow hemophagocytic cells were quantified as 0.5 to 11 per micro liter Median 4.2 per micro liter. Phagocytes showed smaller size single nucleus on one side. Submitting as rings cell plasm showed gray or salmon pink and cell showed disorder fringes.

结果: 符合HPS者30例诊断为HPS早期21例;HPS早期有持续高热峰值≥38.5 ℃、发热期≥4天发病与感染相关;以病毒感染最多见,生化指标TG增高、SF增高;外周血细胞一系或二系减低;骨髓增生活跃或明显活跃,骨髓片(100%)找到吞噬了完整形态的有核细胞、成熟红细胞或血小板的噬血细胞骨髓噬血细胞0.5-11个/ul中位数4.2个/ul吞噬细胞体积偏小多为单个核核偏一侧,可呈印戒样,胞浆灰兰或淡红色,细胞边缘不整齐。

Biochemical indicator such as TG and SF were unregulated while 1 system or 2 system reduced in peripheral blood; Bone marrow hyperplasia showed active or active obviously. Complete morphological nucleated cells mature erythrocytes or platelet hemophagocytic cells were found in 100% marrow slides. Marrow hemophagocytic cells were quantified as 0.5 to 11 per micro liter Median 4.2 per micro liter. Phagocytes showed smaller size single nucleus on one side. Submitting as rings cell plasm showed gray or salmon pink and cell showed disorder fringes.

结果: 符合HPS者30例诊断为HPS早期21例;HPS早期有持续高热峰值≥38.5 ℃、发热期≥4天发病与感染相关;以病毒感染最多见,生化指标TG增高、SF增高;外周血细胞一系或二系减低;骨髓增生活跃或明显活跃,骨髓片(100%)找到吞噬了完整形态的有顾细胞、成熟红细胞或血小板的噬血细胞骨髓噬血细胞0.5-11个/ul中位数4.2个/ul吞噬细胞体积偏小多为单个核核偏一侧,可呈印戒样,胞浆灰兰或淡红色,细胞边缘不整齐。

To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin, and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay, the antibody was purified with rProtein A sepharose fast flow and dephosphorylated antigen membrane. The antibody titrate reached 1:10 000 after purification and its special property was enhanced with absorption treatment of dephosphorylated antigen membrane. In addition, we used rabbit anti-PRAS40 antibody and the phosphorylated PRAS40 (Ser183) antibody to detect PRAS40 expression in several cell lines, including the normal cells HL7702, HEK293, tumor cells HepG2, A549 and S180. There were no quite difference among these cells; otherwise, we observed the decreased phosphorylation level of Ser183 after amino acid withdrawal treatment.

为了制备PRAS40(Ser183)磷酸化多克隆抗体,本实验通过蛋白疏水性抗原性分析设计多肽抗原,用其免疫家兔获得抗血清, ELISA检测其效价为1:10 000; Western blotting法检测发现,通过rProtein A Sepharose亲和层析纯化并经非磷酸化的抗原条吸附处理后的抗体可以明显提高磷酸化抗体的特异性;用PRAS40抗体及PRAS40(Ser183)磷酸化抗体对正常细胞HL7702、HEK293及肿瘤细胞HepG2、A549、S180的检测显示:磷酸化的Ser183在不同细胞中表达差异不显著,而在经细胞饥饿处理的HEK293细胞中却明显观察到了S183磷酸化水平随氨基酸含量降低而减弱的现象。

Four weeks later, in the baicalin group, some spindle-shaped UCB-MSCs began to present shrinkage, with slender processes on the cell edge, and some UCB-MSCs tended to be spherical-, conical-, and triangle-shaped appearance, with many slender processes on the pseudopodia.

细胞形态学观察:脐血单个核细胞培养第2~3天开始贴壁生长,2周左右达高峰,3周后细胞生长达80%~90%融合,此时脐血原始间充质干细胞呈较均一的长梭形。4周后,黄芩苷组原来呈梭形的胞体一部分发生收缩,细胞边缘出现细的突起;一部分胞体逐渐近似圆形、锥形、三角形,伪足有多个细长突起。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。