多细胞的

The long-term opening of mitochondria permeability transition pore results in the release of cytochrome C, apoptosis inducing factors, Ca2+ and other apoptosis factors into cytoplasm, which can destruct the whole cell structure and function by activating the main member of apoptosis protein family——caspase, or destroying the intranuclear chromoplasm independently, or affecting other protein relying on Ca2+, and finally lead to the formation of apoptotic body.

线粒体是真核生物能量和代谢的中心，也是细胞凋亡信号传导途径中起关键作用的细胞器，越来越多的研究表明，在凋亡信号的刺激下，线粒体发生膨胀，其外膜膜电位降低，外膜破裂后释放出膜间隙的诸多凋亡效应因子，在细胞凋亡中起着主开关的作用。

(1) The high throughput and effective Agilent oligomicroarray can screen novel therapy targeted genes by analyzing the DEGs in development and progression of HCC;(2) The development and progression of HCC is a complicated process involving multigenes and multiprocedures;(3) DKK1, as a novel gene, is involved in the development and progression of HCC and may be a new therapy target.

应用Agilent寡核苷酸芯片高通量、高效率地分析原发性肝细胞癌发生发展过程中的基因表达差异，可以筛选新的治疗靶点；原发性肝细胞癌的发生发展涉及多基因、多步骤，是一个复杂的过程；DKK1可能是原发性肝细胞癌发展过程中的新的分子靶点，其表达变化涉及肿瘤进展。

Some physicochemical therapy as well as nake hepatocytes transplantation can't correct metabolic disarrangement because of severe hepatic diseases, ALF is characteristic of more complications and easily resulting in multiple organs dysfunction , and even multiple organs failure as well as higher mortality. transplant technique of encapsulated hepatocytes can inhibit immune rejection, and quarantee hepatocytes survival, the technique provides a new therapeutic method for ALF.

由于肝脏功能较复杂，一些利用物理、化学方法及裸肝细胞移植来代替肝脏解毒功能，但不足以纠正严重的肝病所致的代谢紊乱，易致多脏器功能障碍甚至衰竭，并发症多，死亡率高；而微囊化肝细胞移植技术，既可阻断受体的免疫排斥反应，又保证移植的肝细胞在体内长期存活，为急性肝衰治疗提供新的治疗方法。

Results (1)Astrocytes and oligodendrocytes in mixed culture appeared better growth and differentiation than the respectively purified neuroglial, especially for the oligodendrocytes.(2) There were many pseudopod process among the astrocytes, which appeared centripetal growth mode.(3) The passaged astrocytes kept good abilities of division and proliferation and completely fused after 1 week of growth, and the purity was over 95%.

结果 (1)混合培养的星形胶质细胞和少突胶质细胞生长分化状况均优于纯化后的生长，以少突胶质细胞表现更为明显；(2)星形胶质细胞呈向心性生长，细胞相邻的接触面有较多的伪足；(3)传代培养的星形胶质细胞保持较强的分裂增殖能力，可在1周左右生长至完全融合，其纯度可达95%以上。

Healthy spikes of high-resistant varieties had thicker cell wall and tissue of cortical sclerenchyma, more number of cortical sclerenchyma layers, more number of fibrovascular bundles, smaller area of green subcutaneous tissue in rachis, and theses differences, except number of fibrovascular bundles, enlarged over time. Considering inoculated spikes, cell wall and tissue layers of cortical sclerenchyma displayed different, and to how much extent the relationship between this difference and anti-extension ability of variety had not determined. 6. On the analysis of agronomical characters and molecular marking technology, more than 30 alien anti-Gibberella wheat materials and two mutants coming from this experiment were evaluated their hereditary multiplicity.

实验结果还证明，高抗品种和感病品种的穗轴组织结构确实存在一定差异，在健康穗中，主要表现在高抗品种的皮层厚壁细胞壁和皮层厚壁组织较厚，皮层厚壁细胞层数较多，维管束数目较多，穗轴表皮下绿色组织面积较小，抗、感品种间的差异达显著水平，随发育时间延长，高抗品种的皮层厚壁细胞壁厚度、厚壁组织厚度和厚壁细胞层数增加的幅度较大；在病穗中，主要表现在高抗品种的皮层厚壁组织细胞层数和厚壁细胞壁厚度增加的幅度较大。

Our results showed that the transportation of JA from phloem to xylem occurred mainly through ray parenchyma cells and cambium regions, and that JA could be transported rapidly upwards and downwards through vessel tube and sieve tube.

3H—JA分布在筛管、次生韧皮部的薄壁细胞和射线细胞、形成层区以及导管、次生木质部的薄壁细胞和射线细胞，其中，在韧皮部和木质部的射线细胞及筛管、导管壁周围分布最多。

The size of follicle begin primordial follicle to tertiary follicle(1.5～3mm).The number of mitochondrion increased,and the cristae increased.Gogi body change to type,cortical granule increased,and moved behind cytolemma.The proportion of small,middle and large follicles on ovary were 92.51%(92.51±5.87),4.60%(4.60±4.91) and 2.88%(2.88±2.22) respectively.

卵泡在发育过程中，卵泡细胞形成的突起与卵母细胞始终保持密切的联系：卵母细胞从原始卵泡到直径1.5～3mm的三级卵泡，细胞质中线粒体数量由少到多，线粒体内的嵴不断增加，高尔基体由不典型到变成典型，皮质颗粒由开始形成到形成较多的皮质颗粒，并逐渐地迁移到卵母细胞膜下。

RESULTS: We found that (1) tramadol, similar to the well-knownTRPV1 agonist, capsaicin, significantly increased [Ca2+] i ofTRPV1-CHO cells in a concentration-dependent fashion;(2) itseffect was reversibly prevented by the TRPV1 antagonist capsazepine;(3) repeated application of tramadol resulted in marked tachyphylaxis;and (4) tramadol did not modify [Ca2+] i in control CHO cells.

结果：我们发现以下结果：（1）曲马多和辣椒素这个熟知的辣椒素受体激动剂一样，呈浓度依赖性地显著增加 CHO 细胞 TRPV1内流钙离子；（2）该作用可以被 TRPV1拮抗剂 capsazepine 可逆性地阻止；（3）重复给予曲马多会造成显著的快速耐药性；（4）曲马多不修饰钙对照 CHO 细胞中的内流钙离子。

The transient transfection efficiency was measured by flow cytometry. The stable transfected cells were screened by G418. Results Electrotransfection had the highest transient transfection efficiency (56.8%), with the most masc clones of stably transfection and the least time taken to form big masc clones (20 days). Cationic polymer mediated transient transfection had the lowest efficiency (1.7%), with the least masc clones and the longest time taken to form the big masc clones (30 days). Cationic liposome transfection had a transient transfection efficiency of 39.9% and took 25 days to form the big masc clones.

结果 电转染有最高的瞬时转染效率(56.8%)，稳定转染得到的阳性克隆最多，且从细胞筛选到扩增至得到阳性大克隆所用的时间也最少（约20d）；用阳离子聚合物瞬时转染率低(1.7%)，稳定筛选同样可以得到阳性大克隆，但是数量很少且从细胞筛选到扩增至阳性大克隆所用的时间也最多（约30d）；阳离子脂质体转染效果居中，瞬时转染率为39.9%，从细胞筛选到扩增至得到阳性大克隆所用的时间约25d。

In order to confirm this suspect, we choosed a cultured rat granulation tissue fibroblasts model in vitro and applied RT-PCR and cell-ELISA technology to detect the changes of firbroblasts intrinsic EGF, bFGF, TGFβ-1 with their receptors gene expressions and protein synthesises after stimulated by SP. Our purpose was to explore the possible effects and patterns imposed by SP on fibroblasts intrinsic growth factors and their receptors expressions, which maybe offer theoretical basis for promoting wound healing via improving nervous functions and regulating neuropeptides secretions.

为进一步验证这一推测，同时排除在体多因素干扰，我们采用了一种大鼠肉芽组织成纤维细胞体外培养模型，采用RT-PCR与细胞ELISA技术，检测SP刺激成纤维细胞后，成纤维细胞内源性EGF、bFGF、TGFβ-1及其受体基因表达和蛋白合成的改变情况，探讨SP对成纤维细胞内源性生长因子及其受体表达的影响以及方式，以期为经由改善神经功能、调节神经肽分泌途径促进伤口愈合提供理论依据。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。