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Results demonstrated that the level of ROS was greater in embryo than in aleurone layer, and only very little ROS could be detected in starch. Furthermore, the ROS produced in embryo were mainly localized inside cells but not on cell membrane. By contrast, ROS from aleurone layer were accumulated on cell membrane.

结果表明:胚细胞产生的活性氧最多,糊粉层细胞次之,淀粉细胞几乎不产生活性氧;而且胚细胞产生的活性氧主要定位在细胞内,细胞膜上不产生;糊粉层细胞产生的活性氧主要定位在细胞膜。

Compared with the corresponding BEAS-2B cells, the shapes of cells and nuclears, the shapes and quantity of cell organs were not significantly changed in the 1st, 2nd, 5th passage BEAS-2BNNK cells. The 10th, 15th passage BEAS-2BNNK cells, which had swelled cell and cell organs and abnormal nuclear and enlarged the nucleoli gradually and increased the quantity of the cell organs and activated their function, showed the characteristic of transformation cells. The 20th, 25th passage BEAS- 2BNNKcells, which had obvious aberration of the cell and nucleoli and had cataclasm of nucleoli and decreased cell organs, showed the characteristic of tumor cells.

与BEAS-2B相比较BEAS-2BNNK第1、2、5代细胞及胞核形态、细胞器形态及数量无明显差别;第10、15代细胞出现肿胀,细胞核逐渐变形,出现核畸形,核仁明显增大,边集,细胞器肿胀,数量明显增多,功能活跃具明显的转化细胞特征;第20、25代细胞及胞核明显畸变,出现明显的核碎裂及多个核仁,细胞器明显减少,具明显的肿瘤细胞特征。

The theory is cell kinetics and mechanism of drugs.

主要是根据细胞动力学原理和药物的作用机理分析,如果把药物集中应用,一方面只是一过性的杀灭增殖期的白血病细胞,对静止期细胞影响不大,另一方面对正常细胞造成较大损伤,而如果药物作用于几个细胞增殖周期,对白血病细胞进行几个循环的打击,会清除更多白血病细胞,而对正常细胞损伤较小。

Grew on the normal temperature, it was shown that the deposits of calcium antimonate being the indicator for Ca(superscript 2+) localization mainly concentrated within the vacuoles and intercellular spaces and there was also some Ca(superscript 2+) deposits in cell walls. But when Garyota urens L. was treated by the temperature of 2℃ for 48 h, the level of Ca(superscript 2+) increased in cytoplasm and plasma membrane, but decreased in vacuoles and intercellular spaces considerably. At the same time, the ultrastructure of chloroplasts suffered from chilling: the membrane of chloroplasts had been damaged, the layer of thylakoids was exiguous and unclear, the photosynthetic rate decreased evidently. And when Garyota urens L. was treated by the temperature of 2℃ for 120 h, the deposits of Ca(superscript 2+) mainly concentrated within the cytoplasm, nucleus and plasma membrane and there was also some Ca(superscript 2+) deposits in vacuoles, and the ultrastructure of some cells was simultaneously damaged severely: Chloroplasts structure, vacuole membrane and nuclear membrane had been damaged fully, the structure within the cell had become unclear, and the cell only have respiration.

研究结果表明,未经低温处理的董棕幼苗叶肉细胞,焦锑酸钙沉淀颗粒大量出现在液泡和细胞间隙中,细胞壁中也可见少量沉淀,而细胞基质中则看不到焦锑酸钙沉淀;经2℃ 48 h低温处理后,细胞基质和细胞膜上焦锑酸钙沉淀增加,而液泡和细胞间隙中的焦锑酸钙沉淀则显著减少,并且超微结构已初步显示出寒害的特徵,叶绿体外膜部分破损,类囊体片层稀疏且排列不规则,光合速率明显下降等;经2℃ 120 h低温处理后,细胞间隙内的焦锑酸钙沉淀极少,有的也紧贴在细胞外壁上,而细胞基质和细胞膜上则分布有非常多的焦锑酸钙沉淀,在核基质和液泡中也可见到少量的焦锑酸钙沉淀,并且超微结构遭到了显著破坏,叶绿体结构完全被破坏,核膜与液泡膜严重破损,内部结构模糊,细胞只表现为呼吸作用,不进行光合作用。

Microscope examination:in group a,at 2 weeks,a few inflamatory cells appeared in the holes with many osteoblasts and ostoid appeared around the drilled holes.at 8 weeks,marrow formed in the drilled holes in group b,at 2 weeks,there were a large amount of osteoblasts in the drilled holes and some ostoid formed around thc drilled holes.at 4 weeks,the drilled holes were full of trabeculae.at 8 weeks,the trabeculae matured with the marrow appeared in the intertrabecular space.

组织学结果:2周时a组钻孔区出现少许炎症细胞,边缘出现较多成骨细胞并有骨组织形成,至8周时,钻孔区内形成骨髓组织,只在边缘形成骨小梁结构。2周时b组钻孔区有大量的成骨细胞,边缘有较多骨组织形成,4周时钻孔区内充满新生骨小梁结构,8周时钻孔区内骨小梁成熟,小梁有骨髓组织填充。[结论]骨髓基质干细胞对兔股骨头缺血性坏死有良好的修复作用。

Objective To study the mechanism in which the personality, copying style influenced survival time by changing immunity function in the patients with gastric cancer Methods The Minnesota Multiphase Personality Inventory, coping style questionnaires and immunity function were measured in patients with gastric cancer on late stage, gastric cancer patients with longer survival time, and normal control Results Psychopathic deviate, social introversion subscale scores of MMPI in groupⅡ were significantly lower than that in the other two groups ( P 05) Statistical difference were found between groupⅠand groupⅡ in problem saving factor and help seeking factor of CSQ ( P 05) Natural killer cell level in groupⅡ was significantly higher than in groupⅠ( P 05) There was negative correlation between the NKC and depression, psychopathic deviate, paranoia, psychasthenia, schizophrenia subscale scores of MMPI, there was positive correlation between NKC and SP, coping type in patients with gastric cancer Conclusion The gastric cancer patients with longer survival time are more extroversive and have more mature coping styles Their NKC level is relatively higher The results may be advantageous to improve the prognosis of gastric cancer

作者中文名:王健;邹义壮;唐丽丽;李杰;张东;金山;刘娟摘要:目的研究胃癌患者的生存期与个性特征、应付方式等心理因素,以及免疫功能的关系。方法对胃癌晚期患者、胃癌较长生存期患者及正常健康人分别进行明尼苏达多相个性测验、应对方式问卷、免疫功能测试,并做相关分析。结果(1)MMPI:胃癌Ⅱ组人格障碍评分(42.0±10.5)及社会内向评分(42.2±11.6),低于其他两组[胃癌Ⅰ组为(49.3±11.2)分和(48.3±9.4)分,对照组为(48.6±10.9)分和(39.9±11.5)分;P均<0.05];(2)CSQ:解决问题和求助因子分,胃癌Ⅰ组[分别为(0.68±0.27)分和(0.42±0.18)分]均低于胃癌Ⅱ组[分别为(0.82±0.18)分和(0.65±0.21)分;P<0.05和P<0.01],胃癌Ⅱ组更多采取成熟的应对方式;(3)自然杀伤细胞:胃癌Ⅱ组水平(21.4±9.2)高于胃癌Ⅰ组[(14.2±8.8);P<0.05];而NK细胞水平与MMPI的抑郁(r=-0.35)、人格障碍(r=-0.39)、偏执(r=-0.29)、精神衰弱(r=-0.30)及精神分裂(r=-0.30)等量表呈负相关,与CSQ的解决问题因子(r=0.35)及应付方式类型(r=0.34)呈正相关。结论与胃癌Ⅰ组比较,胃癌Ⅱ组更为外向,负性情绪较少,更多采取成熟的应对方式,其NK细胞水平较高,对胃癌的预后产生了积极的影响。

The cell cycle and apoptosis and the expression of P-pg, bcl-2, P53 in the MG63 and MG63/R cell lines were analyzed by flow cytometry. Results The resistance index of the MG63/R cells to cisplatin was 83.557±4.841. The cells also had resistance to doxorubicin, vincristin, methotrexate and cyclophosphamide. Disordered structure of the MG63/R cells was observed through microscopy. The cells appeared in triangle, polygon and polynucleation. The increase of granular endoplasmic reticulums and apophyses was observed through transmission electron microscopy. The proliferation ability of MG63/R increased significantly, with a low apoptosis index.

结果 经顺铂186d的诱导,建立了MG63/R,其对顺铂的耐药指数为83.557±4.841,对阿霉素、长春新碱、氨甲基蝶吟、环磷酰氨亦产生不同程度的交叉耐药;光镜观察可见MG63/R细胞排列不规则,形态呈三角形、多角形及多核现象;透射电镜显示MG63/R细胞表面突起增加,粗面内质网丰富;细胞周期分析显示细胞增殖能力明显增加而细胞凋亡明显降低;MG63/R细胞 P-pg、bcl-2阳性表达较MG63细胞明显增加,P53表达则明显降低。

The expression pattern of Triae;CYCD2;1 was analyzed by RNA blot hybridization and in situ hybridization , the results revealed that Triae;CYCD2;1 expression is associated with the proliferation tissues, particularly in meristems and leaf primordia.

组织切片结果显示35S::Triae;CYCD2;1转基因拟南芥叶片的栅栏组织和海绵组织分化不明显,多数细胞排列已不规则,茎表皮下多层细胞明显变短,苗端分生组织明显增大且具有较多未分化的小细胞。

The results showed that DpCPV-W could amplify in vitro in Sf21 cell and form normal polyhedra.

研究了文山松毛虫质型多角体病毒在Sf2 1细胞中的离体增殖行为,并进行了空斑试验,结果显示DpCPV W毒株能够在Sf2 1细胞中增殖,也能在Sf2 1细胞上形成空斑,并能产生形态正常的病毒多角体。

Study shows that strengthening body resistance and archaeus herbs have many effects,such as improving and adjusting immunity function,protecting bone marrow,improving haematogenesis function,raising digest and absorb function,improving substance metabolism,preventing gene mutation,inhibiting tumor cell proliferation,inducing tumor cell differentiation or apoptosis,resisting tumor invasion and metastasis,inhibiting formation of tumor vessel and activity of telomerase,etc.It's possibly the result of synergistic effect by multi-pathway and multi-target.

研究表明,扶正培本药物具有增强和调节机体免疫功能、保护骨髓及提高机体造血机能、增加消化吸收功能,改善物质代谢、阻止基因突变、抑制肿瘤细胞增殖、诱导分化、促使肿瘤细胞凋亡、抗肿瘤侵袭与转移、抑制肿瘤血管形成及肿瘤细胞端粒酶活性等多种作用,其发挥的扶正作用可能是多途径、多靶点联合协同作用的结果。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。