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The results showed that there were immature cells (2%-10%) and erythroblast, and puncture of bone marrow revealed myelodysplastic features involving multiple hemopoietic lineages in bone marrow of 19 patients.

结果表明: M6患者的外周血中均可见幼稚细胞(2%-10%)及有核红细胞,骨髓穿刺细胞学检查显示19例伴有多系细胞发育异常,累及二系细胞或三系细胞。

Results:(1)Among 40 rats, 36 rats were successfully established and the rate of success is 90 percent;(2)All the successfully established models demonstrated polydipsia, polyuria and the body weight was not increased. 6 rats suffered cataract after 3 months, and 4 rats died in 6 months;(3)There was an approximately 61% loss of retinal ganglion cells in the central retina and the thickness of retina thinner under microscope ( P 0.01 ).(4) Electroscope changes include the thinner of retina, disorganization of the membranous disc of the rod cells and the thickness of basal membrane of vessal.(5)In normal group, 1 month dibetes mellitus and 1 month treatmen group, there was no expression of ERK1/ERK2 on the retina tissues. In 3 month diabetes mellitus group, the expression of ERK1/ERK2 was positive.

结果:①40只建模大鼠中36只建模成功,建模成功率为90%;②建模成功的大鼠都表现出多饮、多尿、消瘦、体重不增的表现,有6只大鼠在3个月后出现白内障,有4只大鼠在喂养接近至6个月时死亡;③HE染色光镜下6个月大鼠后极部视网膜节细胞层细胞数明显减少,减少约61%(P.01),后极部视网膜明显变薄(P.01);④电镜观察,视网膜变薄,视杆细胞膜盘紊乱,血管基底膜增厚等表现;⑤正常组、糖尿病组和治疗组1个月大鼠视网膜中未见ERK1/ERK2的表达,糖尿病组视网膜组织中3个月时可见少量表达,ERK1/ERK2表达部位为神经节细胞层和内核层;6个月时表达强阳性,部位表达不仅见于内核层、神经节细胞层,色素上皮层也见表达。

Some key techniques related to the close and continuous process were investigated by the application of H9N2 avian influenza virus with Vero cells, such as the susceptibility of cell to influenza virus, virus production with cell microcarrier culture method, cell bead-to-bead transfer, virus production through bead-to-bead transfer, cell culture and virus production with serum free medium, metabolism analysis, and repetitiously intermittent bead-to-bead transfer of cell for virus production to simulate the close and continuous process.

通过使用Vero细胞增殖禽流感病毒H9N2,本文针对封闭连续工艺过程的一些关键技术开展研究,包括细胞对流感病毒敏感性分析、细胞微载体培养生产病毒工艺、细胞珠到珠转移、转移后细胞对病毒增殖敏感性验证、细胞无血清培养生产流感病毒、代谢分析、可模拟连续操作的多次间歇式珠到珠转移培养细胞生产流感病毒等方面。

Results Pores were detected both on electrified erythrocytes and leukocytes with round or ellipse shapes. The erythrocytes often have one or more pores while the leukocytes often have more pores looked like cribble. The rates of perforated cells were increased with the prolonging time of electrification, 5 s with 6% and 1min increased to 40%.

结果 电击后,即可以观察到红细胞与白细胞的细胞膜穿孔,其中红细胞穿孔可为一个或多个,形状多为圆形或卵圆形,白细胞穿孔则为多个,呈筛网状;穿孔细胞数随电击时间延长而增多;电击5s时为6%左右,以后逐渐增加,至电击1min时,穿孔细胞数可达40%左右。

Results: NKA-I cells was first found in the duodenum mucose on the 14th day of fetus, earlier than in the jejunum ( 16th day ) and ileum ( 17th day ), and its Positive reaction and the number of cells increased with further development, after birth the 30th day, it became quite identical to that of adult rat; NKA-I cells were seen in the colon mucosa on the 17th day of fetus, later on the number kept decreasing till grown up stage; No change was observed in the stomach mucosa except that few cells showed up on the 15th day of fetus; No positive cell was found on the oesphagus wall.

结果:NKA-I细胞首先见于胚胎14 d的十二指肠粘膜,早于空肠(胚胎16 d)和回肠(胚胎17 d),其阳性反应强度及细胞数量随发育而增加,30 d时与成年鼠相似;结肠粘膜内于胚胎17 d见多个细胞,但此后数量较少直至成年;胃粘膜于胚胎15 d见个别细胞外,以后无明显变化;食道壁始终未见阳性细胞。

Results:(1)The primary cells in cultured were observed from the edge of the cultured tissues after 10-15 days. The cultured cells were round , oval, fusiform and multiangular.

结果(1)组织块培养10-15天后可见细胞从其边缘向外生长,倒置显微镜下呈梭形、圆形、椭圆形,并有多个突起,电镜下可见表面有较多的微绒毛,细胞间缝隙连接,胞质内见较多次级溶酶体。

Following small injections into the rostral part (namely the dysgranularfield, DI) of the insulpr cortex, retrogradely labeled neuronswere primarily in the parvicellular ventroposterior medial nucleus of the thalamus, the "waist" area of the PBN.

标记细胞有卵圆形、梭形和不规则形,大小平均15u。将HRP注入岛皮质后都,标记细胞见于VPLpc和PBN:VPLpc的标记细胞多位于其中间部,密度较低,以圆形卵圆形细胞为主,梭形和三角形细胞较少见。

Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection. A signal significantly greater than background binding was observed from the third to the fifth round of selection on CHOEGFRGFP1 and CHOK1 cell, but a part of polyclonal scFv bound EGFR specially. About 45% of the selected clones contained a fullsized insert of 1 kb. One unique human antiEGFR scFv (F4scFv) was isolated by analyzing with cell ELISA and DNA sequencing.

结果 经过5轮筛选,细胞裂解液中洗脱出噬菌体效价有500倍以上增长;细胞ELISA结果显示多克隆pscFv与CHOEGFRGFP1细胞和CHOK1细胞均有明显结合,但有部分特异性结合EGFR;菌落PCR显示约45%克隆中含有完整的1kb scFv片断;经细胞ELISA、 DNA测序检测共获得1株EGFR特异性单链抗体,命名为F4scFv。

When inoculating, the modalities ofcells maybe round, triangle or virgulate; and began to be adhesive for 48h; andformation of colony could be formed on day 4-5; Many cells grew in clonalmanner on day 7, and the cultured cells were characterized by large spindle-shapedappearance by the time of 10 days.

刚接种时细胞形态呈圆形、三角形或棒杆状,48h 后细胞开始贴壁,4-5d 可见有集落形成,第7d 形成多个细胞克隆,第10d 细胞呈长梭形,2w 左右可达到基本融合;传代细胞呈均匀的纺锤形,基本上无悬浮细胞。

The basolateral M cell surface is invaginated to form an intracellular central pocket into and out of which lymphocytes and macrophages.

M细胞是一种特化的上皮细胞,多分布于Peyer's结圆顶区的周边,FAE的顶端未见M细胞;其结构有别于相邻的肠上皮细胞,表面没有长而整齐的微绒毛,取而代之的是粗而短的微皱褶,胞质内终末网不发达,溶酶体较少,基底膜向顶部穹隆状突起,形成一&口袋&样结构,其中含有多个免疫活性细胞。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。