多细胞的

Results: TFF2 peptide was distributed in cytoplasm of epithelial cells of the intercalated duct, striated duct and interlobular duct, and positive reaction was found in the cavity, especially near the cavosurface of epithelial cells in the striated duct and interlobular ducts.

结果：TFF2免疫反应阳性物质主要位于闰管、纹状管和小叶间导管上皮细胞胞质，特别是近管腔面更多，纹状管和小叶间导管管腔内亦有阳性物质分布；颗粒区管的少颗粒暗细胞或无颗粒细胞强阳性；多颗粒细胞弱阳性或阴性。

Majority of aderent cells from BM were fibroblast-like cells, and small parts were endothelioid cells. Aderent cells from PB and CB at the fifth generation contained more endothelioid cells and mononuclear and macrophage-like cells besides fibroblast-like cells.

初始培养和传代培养后，骨髓间充质干细胞多为成纤维样细胞，仅有少量内皮样细胞；而传至第5代的外周血、脐带血间充质干细胞除有成纤维样细胞外，还有较多的内皮样细胞和单核－巨噬样细胞。

However among the experimental groups no positive labeling was found in 2h group, positive cells were labeled in SFO and choroids plexus of lateral ventricles in 6h group, especially in ependymal cells of body part of SFO, but no positive cells were found in the other regions.

观察实验组可见：2小时组未发现阳性结果。6小时组在SFO与侧脑室脉络丛，发现阳性细胞，在SFO体部的室管膜细胞阳性较多；其它部位未见阳性细胞。8小时与16小时组在海马、下丘脑、背侧丘脑、脉络丛等多处均有阳性细胞。

The plasticity of NSCs was detected by the differentiating test. HESCs were induced into dopaminergic neurons by means of adding Sonic Hedgehog Peptide and fibroblast growth factor eight (FGF8) early under the condition of mocked microenvironment and different development stage of dopaminergic neurons in vivo ,meanwhile made comparison with traditional method . The marker of dopaminergic neurons was tested by immunocytochemistry and RT-PCR, the content of dopamine and its derivation homovanillic acid were measured by HPLC-ECD.

模拟体内多巴胺神经元分化发育的不同阶段及微环境，采用早期添加音猥因子及成纤维细胞生长因子-8(FGF8)的方法，诱导hESCs定向生成多巴胺能神经元，并与传统的诱导方法相比较，免疫荧光细胞化学和RT-PCR法检测多巴胺能神经元的标志，高效液相色谱法检测诱导后细胞及细胞培养上清液中多巴胺及其代谢产物高香草酸的含量。

The frequencies of micronuclei and multinucleate cells in BEAS-2B cells were significantly increased by DU and these increments of micronucleated cells could be inhibited effectively by DMSO.

贫铀诱发微核细胞、多核细胞增多，DMSO对微核细胞增多有抑制作用，而对多核细胞增多无明显的抑制作用。

The number of DC is more in the cases of those that have more necrotic areas and lymphocytes infiltration.

树突状细胞形态不规则，绝大部分DC分布于坏死灶和汇管区间质中与淋巴细胞伴行，肝细胞内一般不常见，坏死灶越多，淋巴细胞浸润越多的病例中DC细胞也越多。

About 48%(90/194) were below 29 years of age. Ninety-nine of 194 cases had either radiotherapy alone or supplementary treatment with radiotherapy following surgery. We analyzed multiple prognostic factors. The factors of importance included postoperative radiotherapy; female in glioblastoma multiforme; the primary tumor dose above 5000 rads and/or whole brain irradiation in malignant astrocytoma and glioblastoma multiforme; the primary tumor dose above 4500 rads in ependymoma, medulloblastoma and oligodendroglioma; and spinal axis irradiation above 2000 rads in ependymoma and medulloblastoma that produced a better prognosis than in other groups.

男与女之比例为2.7比1，年龄分布自1至81岁，平均年龄33.3±19.3岁，中值年龄为34岁，29岁以下占48%(90/194)，其中99例单独或手术后辅以放射腺治疗，分析同种脑瘤的预后因素，其中多形神经胶母细胞瘤有手术后放射线治疗或女性，多形神经胶母细胞瘤合并星细胞瘤原发肿瘤剂量5000雷得以上或曾全颅腔照射，神经管胚细胞瘤，间胶质母细胞瘤，及室管膜瘤原发肿瘤剂量4500雷得以上，室管膜瘤及神经管胚细胞瘤有脊轴照射2000雷得以上预后均佳，且统计上有显著的差异。

The periostea of both experimental and control side of the mandibular ramus were taken and prepared, 2 of each 5 rabbits in a group were prepared for HE stain detection and 3 for proliferating cell nuclear antigen immunohistochemical detection.Results:1, The newly formed bone was detected on the lateral aspect of mandibular ramus after periosteal distraction. The bone was shaped like a hill. It looked very low and was full of holes at postoperative day 28. With the time of consolidation period lengthened, the newly formed bone matured gradually. X-ray examination showed the new bone shaped like a hill. The average values of new bone height at postoperative days 28,35,42 and 56 were 1.86 + 0.15mm, 2.29 + 0.29mm,3.19 + 0.13mm and 4.70 + 0.45mm. Histological examination of both HE stain and picricacid-fuchsin stain showed the increase in the number of osteoblasts and the change in the orientation of collagen fibers and bone trabecula. There were no significant differences between newly formed bone and original bone on the lateral aspect of mandibular ramus at postoperative day 56 histologically.2 Compared with the control side, the distracted periostea proliferated obviously under the microscope, and the number of periostealcells increased with satiation of cellular nuclear per unit area. The images of PCNA immunohistochemical stain of periosteum showed that the experimental periosteum proliferated obviously after distraction compared with the control side.

结果：骨膜牵张成骨的实验研究南京医科大学硕{学位论文l、骨膜牵张后，可见下领升支外侧的骨皮质上有新骨形成，新骨呈山峰状凸起，术后第28天的新生骨较低平，多孔隙，随着固定时间的延长，新骨逐渐成熟；下领升支前后向切线位X线投照显示新骨呈山峰样隆起；经测量，术后第28、35、42和56天组平均新生骨厚度分别为x.86士0.15mm、2.29士0.29mm、3.19士0.13mm和4.70 土0.45mm；脱钙骨组织的HE染色和不脱钙骨组织的苦味酸一品红染色的组织学观察均显示了新生骨在成骨细胞数量上的增长，以及胶原纤维和骨小梁排列方向上的变化，术后第56天的新生骨在组织学上与原升支骨组织已无明显区别。2、HE染色显示，与对照组相比较，实验侧骨膜增生明显，细胞间排列紧密，单位面积内骨膜细胞数增多，细胞核饱满；骨膜PCNA 免疫组化染色显示，与对照侧相比较，实验侧骨膜在牵张后出现了明显的增生迹象，PCNA阳性细胞分布紧密，单位面积内阳性细胞数较对照组多，靠近骨表面的骨膜中的阳性细胞数更多而且分布更为紧密。

In SW620 cell line, there were more fibroblast-like cells with many microvillus and pseudopodia on the cell surface and loose inter-cells conjunction.

SW620瘤细胞纤维母细胞样细胞较多，表面有较多的微细绒毛和细长胞突，瘤细胞间黏附不紧密。

Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400

图1 SHEE培养在盖坡片上，活细胞单层生长，胞浆较丰富，细胞核大小一致，有核仁×400 图2 SHEE培养细胞细胞核椭圆形，核仁较大，胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状，有伪足贴壁，表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁，呈星状或多角形，有丰富伪足和胞浆突，核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞，胞核和胞浆PI染色呈红色或淡红色，蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞，染色质凝集呈块状，右上角有一固缩细胞核TdT标记×400

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。