多细胞动物

At present there is no effective therapeutics to treat PD except the allopathy. The discovery of neural stem cells in the adult mammalian brain provides a new chance to effect a radical cure on PD. But how to make the limited neural stem cells proliferating enough and differentiating into the dopaminergic neurons which can repair the nigrostriatal pathway and help to eliminate the PD symptoms becomes an urgent problem.

成年动物脑内神经干细胞的发现为根治帕金森病提供了新的细胞源泉，但如何使有限的神经干细胞增殖到足够数量并转化为多巴胺能神经元，使之能够修复黑质纹状体通路，逆转帕金森病临床症状，是目前亟待解决的问题。

Methods The clpE-deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The impact of clpE mutant on the virulence of S. pneumoniae was evaluated in a mouse model. In addition, we also studied the effect of clpE mutant on adherence and invasion of host cells. Real time RT-PCR was used to measure the mRNA expression levels of autolysin A, pneumococcal surface adhesion A, pneumolysin, pneumococcal surface protein A and neuraminidase The clpE gene was replaced completely by erm cassette.

用长臂同源多聚酶链式反应方法失活clpE基因，用PCR、测序鉴定缺失菌株，通过动物实验观察clpE基因缺失株毒力改变情况，同时用细胞实验比较clpE基因缺失株和野生菌对宿主细胞的粘附和侵袭能力，最后用实时荧光定量PCR分析自溶素(major autolysin A，lytA)、表面黏附素A(pneumococcal surface adhesion A，psaA)、溶血素(pneumolysin，ply)、肺炎球菌表面蛋白A（pneumococcal surface protein A， pspA和神经氨酸酶（neuraminidase， nanA）的表达。

Results: The concentrations of sIL-2R, IL-6 and ET in serum in asthma group were higher than that in control group (P.01), Manifestation of the lung tissue under electron microscope: the lung organism of the animal was infiltrated lamellarly by eosinophile granulocyte in the model set.

结果：模型组血浆中sIL-2R、IL-6和ET的含量与正常对照组比较明显增高(P.01)，肺组织光镜和电镜下观察显示：模型组动物的肺组织可见嗜酸性粒细胞片状浸润，Ⅱ型细胞部分线粒体脊突消失、膨胀，板层小体减少，有较多排空现象。

Advance basic research has confirmed that the alkaloids extracted from thunberg fritillary bulb which is widely used in clinical medicine in vitro and in animal experiments have reversed MDR of leukemia. It will increase resistance of acute leukemia cells anticancer drug concentration and lower resistance the expression of Pgp. Pre-Clinical Experimental Research also shows that the conventional chemotherapy combined with the powder of thunberg fritillary bulb for the treatment of acute leukemia. Clinical complete response rate was higher, especially for patients with refractory or relapsed leukemia patients better clinical remission rate. In addition, Caladium particles Slices with chemotherapy in the treatment of RAL Clinical studies also show the Chinese refractory leukemia improve clinical efficacy safe and reliable.

先期基础研究已证实：临床常用的化痰散结中药浙贝母提取的生物碱体外及动物实验均有逆转白血病多药耐药的生物活性，能增加耐药的急性白血病细胞内抗癌药物浓度，降低耐药蛋白Pgp的表达；临床预实验研究也表明，常规化疗方案配合浙贝母粉用于急性白血病临床治疗，其临床完全缓解率明显高于对照组，尤其对难治及复发白血病患者临床缓解率更佳；此外，单药浙贝母颗粒配合化疗治疗RAL的临床研究亦显示中药在提高难治性白血病临床疗效方面安全可靠。

In this study, we isolated and characterized a population of non-human primate adipose tissue stromal cells containing multipotent progenitor cells.

在这个研究中，我们分离和培养灵长类动物猕猴的脂肪基质细胞及其多潜能的前体细胞。

Objective: To approach the drug resistance reversion effect of two kinds of animal origin ω-3 polyunsaturated fatty acids, to human ovarian carcinoma cell line A2780 holding doxorubicin resistance, by the vitro cytotoxicity experiment of human ovarian cancer cell and doxorubicin persister.

目的：通过人卵巢癌细胞及多柔比星耐药株的体外细胞毒性实验，探讨两种动物源性ω-3多不饱和脂肪酸对耐多柔比星人卵巢癌细胞系A2780逆转耐药性的作用。

While age-related muscle atrophy in humans and rodents is well characterized (Grounds, 2002; Welle, 2002; Doherty, 2003; Kamel, 2003; Lynch et al., 2007; Smythe et al., 2008) and satellite cells are known to become mitotically quiescent with age (Schultz et al., 1978; Schultz and Lipton, 1982), age-related changes in activation by stretch or NO were not previously described.

而与年龄有关的肌肉萎缩人类和啮齿动物的良好特征（场地，2002；电波， 2002年，多尔蒂，2003年；卡迈，2003年；证券等。，2007；斯迈思等报。，2008）和卫星细胞已知成为子宫平滑肌静态与年龄（舒尔茨等。，1978年，舒尔茨和Lipton，1982），年龄相关的激活变动伸展或NO以前未描述。

After the establishment of irritable bowel syndrome model, the energy charge of adenosine triphosphate and the ratio of ATP to the total amount of adenylic acid pool in colonic epithelial cells at the ileocecum with better colonization were determined by high performance liquid chromatography.

在建立了IBS动物模型基础上，采用高效液相色谱法对CGC定植较多的回盲部肠黏膜上皮细胞的腺苷酸能荷及ATP与腺苷酸库比值进行测定，以反映细胞活性状态。

Findings in clinical research show that life regenerative substances can activate and culture the healthy organ cells to regenerate and clone, while at the same time, these life regenerative substances can prevent the normal cells from canceration and can induce apoptosis of the cancerous tumor cells. Based on the animal experiments, we have successfully produced the nutritious foods with life regenerative substances according to the national food safety standards. Since Jan 25th 2008, more than 300 cases of cancer patients who had given up the traditional medical treatment accepted our holozoic diet therapy with the life regenerative substances, and remarkable regenerative effects have been obtained. The potential regenerative ability of the organ cells are actived by the life regenerative nutrients, thus the cancer patients continue to struggle against with cancers.

我们研究发现，人体器官的再生物质，在促进正常细胞再生克隆的同时，能阻止细胞被诱导成癌细胞，能使癌细胞凋亡性死亡，从而在进行了动物实验的基础上，将这些再生营养物质制作成符合国家标准的营养食品，于2008年1月25日开始进行了300多例放弃医学治疗的临终癌症病人的全营养食疗养生，获得了再生复原的效果，养生的病人仍能通过再生营养的作用，调动人体的再生潜能，继续与癌症病魔作斗争。

Collagen, a most abundant protein, is widely distributed in skins, tendons, cartilages and constitutes about 25%-30% by weight of all proteins in human or other animal bodies. Collagen structure is characterized by containing domains with repetition of the Gly-Xaa-Yaa prolinrich tripetide and the trimeric collagen triplehelices.

胶原是动物体内含量最多、分布最广的蛋白质，广泛分布于结缔组织、骨骼、内脏细胞间质及肌腔、韧带、巩膜等部位，占人体或其他动物体总蛋白含量的25％～33％。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。