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We optimized reaction system for labelling-antibody in which the optimal amount of the purified anti-Stx2B IgG conjugated with colloidal gold beads was 60μg/mL, the purified antibody for E.coli O157 was 57μg/mL, the optimal pH was 8.2, the size of colloidal gold partical was 20nm, the optimization of stabilizing agent was BSA, the optimization buffer was boracic acid buffer with pH 8.2, the optimization of preserving fluid and eluant was boracic acid buffer with pH 8.2 including 5mM NaCl-1%BSA, confining liquid for NC membrane was 0.01% PBST with 3%BSA, the amount of polyclonal antibody against E.coli O157 and Stx2 conjugated with colloidal gold beads for conjugate pad was 3μg respectively, the amount of anti-Stx monoclonal antibody for test line was 0.1μg and 1μg for E.coli O157, the amount of goat anti rabbit IgG for control line of both GICA were 1μg.

测定了胶体金颗粒的最优化反应体系:胶体金颗粒的大小为20nm;抗体与胶体金溶液结合的最佳pH约为8.2;最佳蛋白结合量分别为抗大肠杆菌O157 IgG为57μg/mL,抗重组Stx2B IgG为60μg/mL;最佳稳定剂为BSA;最佳缓冲液为pH8.2硼酸溶液;最佳金标保存液和洗涤液为5mM NaCl-1%BSA的pH 8.2的硼酸缓冲液;NC膜的封闭液为3%BSA的0.01mol/L PBST;Stx2试纸条和大肠杆菌O157试纸条的质控线上的羊抗兔IgG的多克隆抗体最佳点样量均为1μg,其检测线的抗Stx2的单克隆抗体的点样量为0.1μg、抗大肠杆菌O157的单克隆抗体的点样量为1μg,结合垫上的金标抗大肠杆菌O157和重组Stx2B多克隆抗体点样量均为3μg。

00Any man of mine better be proud of me [00:23.00]Even when I'm ugly he still better love me [00:26.00]And I can be late for a date that's fine [00:29.00]But he better be on time [00:32.00]Any man of mine'll say it fits just right [00:35.00]When last year's dress is just a little too tight [00:38.00]And anything I do or say better be okay [00:41.00]When I have a bad hair day [00:46.00]And if I change my mind [00:49.00]A million times [00:52.00]I wanna hear him say [00:54.00]Yeah, yeah, yeah, yeah, yeah I like it that way [01:01.00]Any man of mine better walk the line [01:06.00]Better show me a teasin' squeezin' pleasin' kinda time [01:13.00]I need a man who knows, how the story goes [01:18.00]He's gotta be a heartbeatin' fine treatin' Breathtakin' earthquakin' kind [01:26.00]Any man of mine [01:33.00]Well any man of mine better disagree [01:36.00]When I say another woman's lookin' better than me [01:40.00]And when I cook him dinner and I burn it black [01:43.00]He better say, mmmm, I like it like that yeah [01:47.00]And if I changed my mind [01:50.00]A million times [01:53.00]I wanna hear him say [01:55.00]Yeah, yeah, yeah, yeah, yeah I like it that way [02:01.00]Any man of mine better walk the line [02:08.00]Better show me a teasin' squeezin' pleasin' kinda time [02:14.00]I need a man who knows, how the story goes [02:20.00]He's gotta be a heartbeatin' fine treatin'Breathtakin' earthquakin' kind [02:28.00]Any man of mine [02:37.00]Let me hear you say yeah, yeah, yeah, yeah, yeah I like it that way [02:44.00]Any man of mine better walk the line [02:54.00]Better show me a teasin' squeezin' pleasin' kinda time [03:00.00]I need a man who knows, how the story goes [03:06.00]He's gotta be a heartbeatin' fine treatin'Breathtakin' earthquakin' kind [03:15.00]Any man of mine [03:22.00]You gotta shimmy shake [03:24.00]Make the earth quake [03:25.00]Kick, turn, stomp, stomp, then you jump [03:30.00]Heel to toe, Do Si Do [03:32.00]'Til your boots wanna break [03:34.00]Til your feet and your back ache [03:36.00]Keep it movin''til you just can't take anymore [03:39.00]Come on everybody on the floor [03:44.00]A-one two, a-three four [03:47.00]Hup two, hum [03:55.00]This is what a woman wants...

比较好的任何我的男人 [00:20.00] 以我为傲 [00:23.00] 即使当我是丑陋的他仍然比较好爱我 [00:26.00] 和我能去一个罚款的日期迟到 [00:29.00] 但是他最好准时任何的 [00:32.00] mine'll 的男人说它仅仅正确适合 [00:35.00] 当去年的洋装仅仅有点儿是紧的时候 [00:38.00] 和任何事我做或说更多是好 [00:41.00] 当我心情不好的时候 [00:46.00] 而且如果我改变我的想法 [00:49.00] 一百万乘 [00:52.00] 我想要听到他说 [00:54.00] 是的,是,是的,是,是的我那样喜欢它任何我的比较好的散步男人线的 [01:01.00] [01:06.00] 最好给我看 teasin'squeezin' pleasin'有一点时间 [01:13.00] 我需要一个知道的男人,故事去的方式 [01:18.00] 他是必须是 heartbeatin'罚款 treatin'Breathtakin' earthquakin'类型任何我的男人 [01:26.00] [01:33.00] 涌出任何我的男人比较好的不同意 [01:36.00] 当我说另外的一个女人的 lookin 的时候'比较好的超过我 [01:40.00] 和当我烹调他晚餐,而且我燃烧它的时候黑色的 [01:43.00] 他比较好的发言权, mmmm,我像那一样喜欢它是的 [01:47.00] 而且如果我改变了我的想法 [01:50.00] 一百万乘 [01:53.00] 我想要听到他说 [01:55.00] 是的,是,是的,是,是的我那样喜欢它任何我的比较好的散步男人线的 [02:01.00] [02:08.00] 最好给我看 teasin'squeezin' pleasin'有一点时间 [02:14.00] 我需要一个知道的男人,故事去的方式 [02:20.00] 他是必须是 heartbeatin'罚款 treatin'Breathtakin'earthquakin'类型任何我的男人 [02:28.00] [02:37.00] 让我听到你说是的,是,是的,是,是的我那样喜欢它任何我的比较好的散步男人线的 [02:44.00] [02:54.00] 最好给我看 teasin'squeezin' pleasin'有一点时间 [03:00.00] 我需要一个知道的男人,故事去的方式 [03:06.00] 他是必须是 heartbeatin'罚款 treatin'Breathtakin'earthquakin'类型任何我的男人 [03:15.00] [03:22.00] 你车轮摆动必须摇动吗 [03:24.00] 制造地球地震 [03:25.00] 踢,旋转,跺脚,跺脚,然后你跳跃 [03:30.00] 尾随以趾踏触, Si 做吗 [03:32.00]'胡麻你的长靴想要打破 [03:34.00] 胡麻你的脚和你的后面痛 [03:36.00] 保存它 movin''胡麻你就是能不再轮流 [03:39.00] 在地板上受到在每个人身上的影响 [03:44.00] 一-三,一-七 [03:47.00] Hup 二,嗡嗡声 [03:55.00] 这是一个女人想要的。。。

Therefore, this paper proposes an approach which builds a complete model including thread interactions and "out of order" execution in a single thread.

因此,本文提出了一种建立包含多线程交互及线程内代码乱序执行的完整模型,并利用模型检测工具进行穷举检测的算法。

One of the most common ways to describe a wood's tonal properties is in terms of its frequency range,which is often broken down into low-end frequencies,mid-range and high-end frequencies.picture it as a visual spectrum,as we've done in the chart to the right,with the lower frequencies on the left and the higher frequen-cies on the right.the graph line for each wood visually depicts its general tonal range.rosewood and ovang kol,for example,tend to resonate with more low-end frequencies,whereas koa,cocobolo and maple tend to sound brighter from having more top-end frequencies.note also rosewood's"scooped"midrange and ovang-kol's fuller midrange.the doted lines for walnut and koa denote the expansion of low-end frequency range as the guitar opens up after a period of playing it.

最常见的方式描述了木材的声音特性是在其频率范围内,这往往是细分为低端频率,中端和高端frequencies.picture它作为一个视觉频谱,因为我们'维生素E这样做的图表,以权,以较低的频率在左边和更高的频率,种上right.the图线的每一个木材视觉描绘的一般音调range.rosewood和ovang科尔举例来说,往往会产生共鸣更多的低端频率,而兴亚, cocobolo和枫树往往从健全光明有更多的高端frequencies.note也花梨木的&收购&的中端和ovang -科尔的充分midrange.the doted线的核桃和兴亚指扩大低端频率范围内的吉他开辟了一段时间后,打它。

I used 165/46 litz instead of larger litz because regenerative radios tend to be "Q multipliers". I thought that the 660/46 wire would make the radio too sharp and perhaps unstable.

我用了165/46李兹线而没有用更多股数的原因,是因为再生电路有&倍Q&作用,我想660/46李兹线可能使得谐振曲线太过尖锐而使得电路变的不稳定。

And -- are perfectly safe to use in single-threaded servers, because even if the client app is multi-threaded and makes method calls from different threads, the COM library serializes method calls into our server.

从不同的线程++和-是十分安全使用单线程服务器,因为即使客户端应用程序是多线程,并使方法调用,COM库序列化为我们的服务器方法调用。

D be provided with one or more raised longitudinal ridges, if a parallel conductor flexible cord.

若是平行的导体软线,就要配上一个或更多纵向的脊线。

He says the sail will consist of up to 100 aluminum or copper alloy wires—each about 12.4 miles, or 20 kilometers, long and 20 microns in diameter (one micron equals about one four hundred-thousandths of an inch)—attached to reels that can adjust wire length. Each wire would be charged at up to 20 kilovolts via a solar-powered electron gun attached to the sail.

据Janhunen介绍,这种空间帆由多达100根铝或铜合金线组成——每根大概12.4英里(20公里)长,直径为20微米,这些线都绕在一个轮轴上因此可以调节线的长度。

The thread geometry includes single thread active devices and multiple thread devices.

线的几何形状包括单线有源器件及多线器件。

A mathematic model of cord tension of any lamina is built up by calculating the stiffness model under inflation and static loading case of tire also.

该模型表明帘线受力特征与轮胎加载条件、轮胎断面的形状和尺寸、帘线的规格、帘线的铺设角和排列间距、带束层的结构形式、带束层的宽度等诸多因素的耦合作用密切关联。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?