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Pairing of homologous chromosomes commences at one or several points on the chromosome and is clearly seen during PACHYTENE of meiosis I.

同源染色体两两成对平行靠拢,这一现象也称联会,联会发生在染色体的一点或多点上,可以在减数第一次分裂的粗线期观察到。

At zygotene,t he SC formation initiated at multiple sites in each bivalent but the regions nea r telomeres were preferred.SCs were shortened with development of SCs.Initiation of new SC and extension of existing SC occured simultaneously,and pairing in di fferent bivalents of a nucleus was not synchronised.

结果表明,小麦SC以多点式起始方式于偶线期开始形成;随SC的发育,新的SC形成和已有SC片断的延伸并存;此外,在同一核内不同二价体之间,染色体配对和SC形成并不同步;SC成熟于粗线期,而以破碎方式解体,消失于双线期。

The signals look like noncontinuous beads on the DNA fiber,which consist of multi-copy and arrange tandemly.Fiber-FISH results with 45S rDNA probe showed that an average signal length is about 3~11μm in many tandem copy sequence(measure number,n=8),so we estimated the size of each copy to be approximately 11~30kb in cotton genome.There are dual signals on each end of the mitosis metaphase chromosomes and pachytene chromosomes hybridized with telomere DNA probe.The signals also look like non-continuous beads on the DNA fiber,the length is about 1~9μm,so we estimated the size is about 4~27kb.The signals almost covered the whole mitosis metaphase chromosomes and pachytene chromosomes of G.arboretum Shixiya 1 hybridized with gDNA probe,the euchromatin zone and heterochromatin zone were identified clearly on pachytene chromosomes,and the signals also look like non-continuous beads.Two BAC clones 150-D-24 and 182-F-9 in DNA BAC library of Pima90 were selected as probe to hybridize with mitosis metaphase chromosomes,pachytene chromosomes and DNA fiber of G.raimondii.

棉花FISH技术系统的应用研究。45S rDNA在亚洲棉石系亚1号中期和粗线期染色体上有两对大的信号,在DNA纤维上信号为非连续的念珠状,多个拷贝串联排列,每个拷贝长度大约在3~11μm,推测每个拷贝的实际长度为11~30kb;端粒序列在亚洲棉石系亚1号中期和粗线期染色体端部都有双点信号,在DNA纤维上信号也为非连续的念珠状,不同染色体上信号长度大约在1~9μm不等,推测实际长度为4~27kb;gDNA信号几乎布满整个亚洲棉石系亚1号中期和粗线期染色体,在粗线期染色体上能明显区分出常染色质区和异染色质区,DNA纤维上的信号均为非连续的念珠状。

The results showed that: at metaphase I, the homoeologous chromosome pairing among different F1 hybrids ranged from 2.0 to 11.4 bi-valents formed by homoeologous chromosomes per pollen mother cell, and very few multivalents, and even very few bivalents were formed by two chromosomes within one genome rather than homoeologous chromosomes in some PMCs; at anaphase I, all biva-lents were disjoined and most univalents were divided.

结果表明在中期I阶段,这些杂种一代的近缘染色体联会变化很大,每个花粉母细胞中二价体形成的数目从平均2个到11.4个不等,甚至在某些花粉母细胞中,还发现极少的多价体和非部分同源染色体所形成的单基因组内二价体;在后期I时,所有的二价体分离,同时多数单价体也分离,分离的二价体和分离的单价体都移向两极,从而形成两组染色体;因为这时完整花粉母细胞中分离的二价体在两组染色体中总是对应出现,从而根据半二价体上染色体重组的位置可以分析在二价体的四分体时期发生在非姊妹染色体之间的多种染色体交换类型,如单交换、三线双交换、四线双交换、四线三交换和四线多交换。

When applied the spiny eel X chromosome library probes to the chromosomes of the two fish, different signals were distributed: in Siniperca chuatsi, besides some little signals on lot of autosomes, a large bright signal region was detected on the 8th chromosomes, which represented a synteny of spiny eel X chromosomes; but in Channa argus, no distinct synteny was found.

当以刺鳅的生物素标记X文库探针对鳜鱼和乌鳢的有丝分裂染色体进行染色体涂绘后,经过核型分析和统计分析表明,在这两种鱼的染色体上的杂交信号分布是不同的:在乌鳢中,除散在信号外,在其第八号染色体近着丝粒部位存在着一段较大的信号结合区域,应是与刺鳅的X染色体同源的保守同线群,而在鳜鱼中,只有散在信号分布于多条染色体上,没有明显的保守同线群存在。

Under normal temperature (25-30℃), the process of pollen mother cell meiosis is normal; The most of chromosomes become bivalents which like "V","O","Y","X", sticks and dots, and mainly like dots,"V" and "O"; and a few of univalent (0.79% of Tainong 1 and 0 of Irwin) and multivalent (4.76% of Tainong 1 and 3.57% of Irwin) had been observed.

常温(25~30℃)条件下,花粉母细胞减数分裂过程较为正常,双线期染色体主要形成二价体,二价体构型以&V&型、&O&型、&Y&型、&X&型、点状和棒状等形式存在,并以点状、&V&型和&O&型较多见,出现少量的单价体和多价体,其中台农1号为0.79%和4.76%,Irwin为0和3.57%;同时仅在少量中期Ⅱ和后期Ⅱ花粉母细胞中观察到落后染色体。

Based on this result, it is concluded that the change of acetylation level at the loci of hsp, mediated by histone deacetylase inhibitors, exertsimportant functions in hsp gene transcription.3, After immunolabeling with anti acelated-lysine monoclonal antibody on the polytene chromosomes of heat shocked flies, fluorescence signals were detected at the hsp loci.

3,利用抗乙酰化赖氨酸的单克隆抗体对热激处理后的果蝇幼虫多线染色体进行的免疫荧光实验结果表明在热休克诱导的hsp70基因的表达中存在乙酞化修饰。

By the method of SDS-PAGE, the main proteins of nuclear, matrix in salivary gland were proteins of 67kd, 66kd, 43kd, 38kd and 35kd Comparing the NHP composition of the nuclear matrix to that of the polytene chromosome, it can be seen that the nuclear matrix and the polytene chromosome shared many common NHP components, such as 43kd, 38kd and 35kd proteins.

通过SDS-聚丙烯酰胺凝胶电泳看到,果蝇唾腺细胞核基质的主要蛋白成份为67kd、66kd、43kd、38kd及35kd等多肽。与多线染色体骨架的蛋白成份比较,发现它们共同具有43kd、38kd及35kd等蛋白质。

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