多糖

The mechanism of PPS accelerating IEC-6 cells migration was also researched with gene-chip technique. The Altas Clontech Rat cDNA Chip which consisted of 1176 spots representing different functional categories such as heat shock/stress, cell cycle, transcription factors, hormones, metabolism, etc was used. Result showed in cell migration model of IEC-6 cell line, compared with control group, expression of 38 genes in total 1176 genes varied in cells treated by PPS, including 21 genes up-regulated and 17 genes down-regulated. Date analyze showed the mechanism of PPS accelerating IEC-6 cells migration mainly related with promoting the numbers and activity of cellular ion channel, especially 〓 channel.

从以上实验结果中我们可以得出以下结论：四君子汤总多糖具有促进IEC-6细胞增殖、迁移、粘附的作用；四君子汤总多糖无论是对IEC-6细胞增殖、或者迁移的作用均优于各单味药多糖，可能是各种单味药多糖综合作用的结果；多糖可能是四君子汤"益气健脾"药效的主要成分；小肠上皮细胞可能是四君子汤总多糖的作用靶点之一，提示临床采用四君子汤及"益气健脾"法治疗"脾虚证"的疗效机制可能与增强机体的整体免疫和肠道粘膜免疫功能有关。

Considering the experimental results, we start from plant and animalcule polysaccharides'structure, pharmacological activity and the structure activity relationship of polysaccharides, with emphasis on polysaccharides'macromolecule's action in solution、chain conformation 、the characters of solution、molecular weight and polysaccharides'interaction.Combining other reports on polysaccharides compounds, we have analyzed the mixing rules of polysaccharides , and brought out five possible approaches of polysaccarides mixing.

结合实验结果，本论文以植物多糖、微生物多糖的结构、药理活性以及构效关系为基础，以多糖高分子在溶液中的行为、链构象、溶液性质、分子量以及多糖在溶液中的相互作用为重点，结合大量的复合多糖研究报道，分析归纳了多糖配伍复合的规律，提出了5 个多糖复合的可能途径。

Chapter 4 Capillary Zone Electrophoresis with Amperometric Detection forComposition Analysis of LaminarinThe composition of laminarin was firstly determined by analyzing its hydrolysis monosaccharides with capillary zone electrophoresis - amperometric detection.

第四章毛细管电泳一安培检测法测定昆布中多糖成分本论文首次用毛细管电泳一安培检测法测定了昆布多糖水解单糖的成分，实现对(来源：Aa4BC9b2b论文网www.abclunwen.com)昆布多糖的测定。在选定的最佳条件下，昆布多糖水解产物岩藻糖、半乳糖、葡萄糖、甘露糖、木糖在20min内实现完全分离而且在铜电极上有着很好的电流响应。通过对昆布多糖中水解单糖成分含量的计算显示该多糖中岩藻糖、半乳糖、葡萄糖、甘露糖、木糖的摩尔比为

Give birth to content and food engineering according to university of industry of project technology chief, Dalian courtyard dean Zhu Bei osmund professor introduction, this project developed microtherm vacuum to permeate first flavor to style of technology, stage warms up and cool antiseptic processing technique, in the treatment that uses at eating abalone product namely, solved original feed the difficult problem of technology of not easy pilot such as abalone product appearance, mouthfeel, aroma, color namely, built the manufacturing technology system that feeds abalone namely, to develop other ocean shellfish fed a product to lay good foundation namely; Use first from dissolve enzymatic with outside polysaccharide of abalone of extraction of method of source enzymatic compound, raised the recovery rate of polysaccharide greatly; Use enzymatic law and the method that Sevag law photograph combines, solved effectively take off the difficult problem of miscellaneous albumen in dividing abalone polysaccharide, raised polysaccharide purity, maintained polysaccharide active, the technical system of the preparation that built abalone polysaccharide, detached, rarefied, analysis, draw lessons from goodly to developed polysaccharide of other and marine animal to offer.

据项目技术负责人、大连工业大学生物与食品工程学院院长朱蓓薇教授介绍，该项目首次开发了低温真空渗透调味技术、阶段式升暖和冷却杀菌加工技术，并应用于即食鲍鱼产品的加工中，解决了原有即食鲍鱼产品外形、口感、香气、颜色等不易控制的技术难题，建立了即食鲍鱼的生产技术体系，为开发其他海洋贝类即食产品奠定了良好的基础；首次采用自溶酶与外源酶复合的方法提取鲍鱼多糖，大大提高了多糖的提取率；采用酶法与Sevag法相结合的方法，有效地解决了脱除鲍鱼多糖中杂蛋白的难题，提高了多糖纯度，并保持了多糖活性，建立起了鲍鱼多糖的制备、分离、纯化、分析的技术体系，为开发其他海洋动物多糖提供了良好的借鉴。

The research mainly reveals, the mixing principle to yield the synergistic effect of different polysaccharides mixed according to Chinese traditional medicine's rules; The principle of polysaccharides from different resources but with the similar main-chain and the same medicine active characters yielding synergistic effect; The principle of polysaccharides from different resources and different structure but with the same medicine active characters yielding synergetic effect; The principle of polysaccharides from the same resource and with the semblable main-chain structure and the same pharmacological active characters yielding synergetic effect; The principle of polysaccharides of different pharmacological active characters and with its ingredients synergic improving pharmacological effect.

根据中药复方的组方进行多糖复合；以不同来源、相似的主链糖单元、相同的药理活性多糖进行多糖复合；以不同来源、不同结构、药理活性相同多糖产生协同效应来进行多糖复合；以相同来源、相似主链结构以及相同药理活性多糖产生协同效应来获取复合多糖；以具有不同的药理活性多糖进行配伍，各有效成分的作用相互协同互补，扩大药理作用范围来获取复合多糖的配伍原则具有一定的可行性。

30U、P30W、P30S1 and P30S2 were isolated from PIP30, and P60U、P60W、P60S1 and P60S2 were isolated from PIP60, and P1W、P1S1 and P1S2 were isolated from PIWE1-10, and P10W、P10S1 and P10S2 were isolated from PIWE10-100, and P100U、P100W、P100S1 and P100S2 were isolated from PIWE100 using DEAE Sepharose F. F. The proliferation rates of lymphocyte in vitro of each polysaccharide fractions obtained during the isolation and purification were detected in order to observe their immune activities. The results showed that crude polysaccharides both obtained from ethanol precipitation and ultrafiltrate had the promoting effects on lymphocyte proliferation in various degrees. P30U、P30W、P30S1、P30S2、P60U、P60W、P60S2、P1S1、P1S2、P10S1、P10S2、P100U、P100W、P100S1 and P100S2 had the promoting effects on lymphocyte proliferation.

IP30经阴离子交换层析(DEAE-Sepharose Fast Flow)得到分级多糖P30U、P30W、P30S1和P30S2，PIP60经阴离子交换层析得到分级多糖P60U、P60W、P60S1和P60S2，PIWE1-10经阴离子交换层析得到分级多糖P1W、P1S1和P1S2，PIWE10-100经阴离子交换层析得到分级多糖P10W、P10S1和P10S2，PIWE100经阴离子交换层析得到分级多糖P100U、P100W、P100S1和P100S2；采用体外刺激淋巴细胞增殖实验对离子柱分级分离的多糖进行活性实验表明P30U、P30W、P30S1、P30S2、P60U、P60W、P60S2、P1S1、P1S2、P10S1、P10S2、P100U、P100W、P100S1和P100S2是有活性的。P60W、P60S1、P1S1、P10S1和P100S1经凝胶柱(Sephacryl S系列材料)层析获得五个纯多糖组分P60w1、P60s1、P1SP1、P10SP1和P100SP1。

The results indicated that GLP was a linear (1→3)β-D-Glcp main chain linkage. Its monosaccharide component was predominantly composed of D-Glc, and small amount of galactose, mannose, xylose and idose, residues of branches terminated with substituted at 1→6 by a small number of singleunit β-D-Glcp side-chains, it's also observed that the (1→3)-linked β-D-glucan contained a triple-helical conformation, which was composed of a repeating unit with a structure as below:.

结果表明：灵芝多糖含有三螺旋体构型，GC-MS分析灵芝多糖的主要单糖组分为葡萄糖，还有少量的半乳糖、甘露糖、木糖和艾杜糖，IR及1HNMR分析多糖为β-构型，HIO4氧化、Smith降解和甲基化分析表明：多糖主要为(1→3)糖苷键连接构型，并伴有少量的1→6位支链键连接的结构，灵芝多糖是由D-葡萄糖单元通过β-(1→3)糖苷键连接葡聚多糖，其主要构型特征为(1→3)β-D-线性连接的骨架结构。

By this method, the productivity of 3. 18g/l extracellular polysaccharides was obtained. The optimum extraction method was obtained by RSA. It was determined as follows: 91. 4 ℃, 2. 9h, the weight ratio between mycelia and water was 1 to 3. The productivity was 12. 38% of dry mycelia. Set the purifying methods of Grifola frondosa: Precipitated polysaccharides part by 60% ethanol→removed protein by Sevag method→removed coloring matter by H〓O〓→removed salts by dialyse→DEAE-cellulose column chromatogram→ purified polysaccharides groups.

对于胞内多糖的提取，采用湿菌体经捣碎再高压破壁的方式破碎菌体细胞，采用响应面分析法得出优化后的提取条件为：提取温度91.4℃，提取时间2.9h，料水比1：3，此提取条件下胞内粗多糖的率为干菌体重的12.38％建立了灰树花多糖分离纯化的技术路线 60％乙醇沉淀的粗多糖→Sevag法脱蛋白→H〓O〓脱色→透析脱盐→DEAE-纤维素层析分离→多糖组分。

In order to select monomer of Chinese herbal polysaccharide with better immunity, four CHPs, APS, ABPS, IRPS and DOPS at six doses were added in the splenic lymphocyte of chick in vitro, and cultured 48 h. The splenic lymphocyte proliferation was determined with the MTT method.

为了筛选免疫增强作用较好的单味中药成分，将6种浓度的黄芪多糖、牛膝多糖、板蓝根多糖、山药多糖等4种中药多糖分别加入到体外培养的鸡脾脏淋巴细胞中，培养48h后，用MTT法测定淋巴细胞增殖的变化。

The results show that though the strain is growing slower in the nitrogen-free medium than in the nitrogen-containing medium, the content of capsular polysaccharides produced by the strain in the nitrogen-free medium is higher than those in the nitrogen-containing medium, the content of capsular polysaccharides produced by the strain in the nitrogen-free medium is higher than those in the nitrogen-containing medium. The capsular polysaccharides produced by the strain in the culture which contains different mineral powders will reach the highest content on the third or the fourth day in its growing period. The strain's capability of releasing potassium from k-feldspar and biotite in the nitrogen-containing medium is higher than that in the nitrogen-free medium because in the nitrogen-free medium, the strain and its production of glucoprotein are less than those in the nitrogen-containing medium.

结果表明，尽管该菌在无氮培养条件下的菌体数量远小于有氮培养条件，但无氮培养条件下所提取的细菌多糖多于在有氮条件下培养所提取的细菌多糖；胶质芽孢杆菌在以添加钾长石粉或黑云母粉制作的无氮培养基中生长可形成大量多糖，采用丙酮法进行细菌培养液多糖提取，发现细菌培养的第3天所提多糖含量最高；胶质芽孢杆菌在以添加钾长石粉或黑云母粉制作的有氮培养基中生长亦可形成较多的多糖，且在细菌培养的第4天所提多糖含量最高；胶质芽孢杆菌在有氮条件下对含钾矿物的释钾率高于在无氮条件下的释钾率，这可能与该菌在有氮条件下生长更快、可产生较多菌体细胞有关。

By using competitive adsorption of activated carbom to remove unfavorable influence of carbon in carbonaceous gold concentrate on leaching process, the leaching rate of gold concentrate can be improved to 88% as compared with 10%by direct cyanidation.

本文论述了利用活性炭竞争吸附作用，消除含炭金精矿中炭在浸出过程中的影响，使金精矿的浸出率可由直接氰化的10％提高到88％。

The only thing you're ever gonna be king of is King of the Stupids!

你只配做傻瓜的国王！