多粒的

Results: The light microscope pictures of group A(no ［α-32P］ ATP in the perfusate) showed that there was no specially labeled silver pellet in or out of rat liver cell. But the light microscope pictures of group B (37 MBq ［α- 32P］ATP in the perfusate) showed that distribution of numerous ［α-32P］ATP autoradiographic silver pellets in rat liver cell, and no autoradiographic silver pellet was found in the hepatic sinus and vessel.

结果：灌流液中不加［α-32P］ATP的A组光镜照片显示大鼠肝细胞内外均未见特异性标记银粒显影，而灌流液中含37 MBq［α-32P］ATP的B组光镜照片显示大鼠肝细胞内可见多个大小不等的［α-32P］ATP自显影银粒分布，而肝窦及肝小叶中央静脉等血管内则几乎无黑色自显影银粒分布。

In this paper, an escherichia coli-saccharopolyspora erythraea shuttle vector containing the erya promoter region was constructed using the enhanced green fluorescent protein gene as a reporter. the shuttle plasmid was transformed into sac.erythraea a226 and streptomyces lividans jt46 by peg mediation, respectively. fluorescence microscopy confirmed that the egfp was expressed in both strains.

本文克隆了erya基因的启动子perya，以绿色荧光蛋白基因为报告基因，构建了大肠埃希菌-糖多孢红霉菌穿梭型质粒。peg介导原生质体转化法将穿梭型质粒分别转入糖多孢红霉菌a226与变铅青链霉菌jt46，荧光显微镜检测发现，此启动子在两菌株中都具有功能。

In the present paper, a theoretical model of the mobility for multidisperse particles is developed. The present model is the extension of the previous theoretical model and the semi-empirical model for the monodisperse partcles. This model includes the influence of particle size distributions and is suitable for all particles with different materials and with different size distributions.

通过理论分析，本文提出了多分散性尘粒电迁移率数学模型；此模型是典型的电迁移率模型和单分散性尘粒电迁移率半经验模型的扩展，它考虑了粒度分布的影响，适用于各种物质的尘粒和各种粒度分布的尘粒。

Moreover, we compared the transfection efficacy of small unilamellar vesicles and multilamellar vesicles.

本实验并使用了不同种类的正电性脂质、微脂粒中不同莫尔比的正电性脂质及其助手脂质，不同组成的小微泡单层微脂粒及多层微脂粒去比较其基因转殖效果。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

The results indicate that the mass size distribution of fly-ash particles in the inlet of the WFGD system is typically disturbed bimodally, the peaks are approximate at 1 and 3 μm. And the shape of particles is regular sphericity, the mass ratio of PM2.5 to PM10 is about 0.434. The total mass concentration is about 85 mg/m3. In the outlet of the WFGD system, the mass size distribution of fly-ash particles is also bimodal. The proportion of fine particles increased, the mass ratio of PM2.5 to PM10 is about 0.764. Some fine particles are conglutinated and formed irregular agglomerate. The total mass concentration is under 23 mg/m3, the removal efficiency of total particles is 74.5%. The size removal efficiency is decreased observably with the range of particle size decreasing.

结果表明，WFGD系统入口飞灰质量粒径呈典型的双峰分布，峰值分别在1和3 mm处，颗粒多呈规则球形，PM2.5与PM10质量比为0.434，飞灰总浓度约为85 mg/m3标准状态；出口处飞灰质量粒径分布也呈现双峰性，其中细颗粒比例增大，PM2.5与PM10质量比为0.764，细颗粒间相互聚集粘连形成不规则的块状结构，飞灰总浓度在23 mg/m3以下，总飞灰的脱除效率为74.5%，分级脱除效率随粒径减小而明显下降。

Porous asphalt concrete is composed of asphalt mastic, coarse aggregate, thin aggregate, stuff and admixture. Such a combination is expected to fully integrate these materials. In this way, the function of interlocking among particles can be performed, a firm net of porous asphalt framework formed and the ability to resist rut and wear developed. The different mixture results in different effects; the quality is so complicated that the factors that cause damage are multiple. Therefore, it is bothersome to choose one material that can be adjusted to various conditions everywhere, such as the traffic volume, load and the weather. The current study mainly investigates and analyzes the main factor that caused flaws after the roads paved with porous asphalt concrete have been open. The analysis aims to search for the best solutions, promote the quality, prolong its availability and finally reach the purpose of complete use.

多孔隙沥青混凝土依其组成材料由沥青胶泥、粗粒料、细粒料、填充料、添加物等组成，目的是希望藉由以上材料的组合能充分接触，发挥颗粒间互锁作用，形成牢固的连锁网的沥青混凝土架构，发挥粒料本身良好的抗车辙及抗磨损之能力，因添加物的不同所发挥出的效果亦不同，其性质甚为复杂，造成损坏之因素亦属多重性，要择其适用於各地迥异之各种条件，如交通量、荷载及气候环境等因子，极为繁琐，本研究主要探讨及分析已铺筑之多孔隙沥青混凝土铺面，经通车后产生之缺失主要因素，以寻求其最佳解决方案，提升品质与延长使用寿命，进而达到全面使用。

For tablets (including special-shaped), pills, capsules and other medicine plastic bottles, the main products include: automatic grounds for bottles, bottle-automatic machines, several tablets of electronic, mechanical few tablets of machines, variable frequency screen type machinery A few tablets, automatic Cypriot paper machine, automatic machine cap, electromagnetic induction-sealing machine, sizing automatic labeling machine, sticker labeling machine, Cypriot paper - cap - sealed the multi-one free packaging and other components The joint automatic packaging production line, its technical performance of similar products in the country with lead.

针对片剂、丸剂、胶囊等药品的塑瓶包装，主要产品有：自动理瓶机、自动清瓶机、电子数粒机、机械数粒机、变频筛动式机械数粒机、自动塞纸机、自动旋盖机、电磁感应封口机、上浆自动贴标机、不干胶贴标机、塞纸-旋盖-封口多工位一体联合包装机等自由组成的联合式自动包装生产线，其技术性能在全国同类产品中具有领先水平。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

YR-cohesion chromosome model will give a better answer to the questions such as exchange,centromere,holocentromere,synaptonemal complex,polytene chromosome,puff,lampbrush chromosome,chromosome banding,non-segregation of sister chromatid,pollentube pathway and biological evolution.

YR-染色体模型能自然、合理地解释所有遗传现象，如交换、着丝粒、全身着丝粒或弥散型着丝粒染色体、同源染色体联会及联会复合体的中央区、多线染色体与膨突、灯刷染色体、染色体分带、姊妹染色体由前期到中期不分开、花粉管会导入外源遗传物质、高等生命是怎样从原始生物进化而来的等等。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。