多粒的

Now every thing we offer is to go through a delicately designed procedure, even a coffee cup, a desert bag, or a coffee bean. This procedure is priceless and is the greatest achievement in the six-year history of Firstar. We have learned the westerners' work style and life attitude: perseverance and responsibility.

一只咖啡杯，一个甜品袋，一粒咖啡豆，都是要经过那么多讲究的流程，这个过程是无价的，也是我所说的这是浮士德6年来最大的成长，从浮士德咖啡我们学习到了西方人的工作和生活态度，对一件事的坚持和责任。

It was Moran's second goal in four days after he netted a last-gasp equaliser in the 3-3 draw with Manchester United at Old Trafford, and it proved to be the defender's most prolific season for the Reds, netting seven goals in 39 appearances, including five penalties

这是莫兰的四天内的第二粒进球，上一个是在老特拉福德与曼联的3：3平局中打进红军最后扳平的一球。这个赛季是这位防守球员在红军生涯中最为多产的一个赛季，39次出场进7球，包括5个点球。

Suffice to say: goals in every major domestic final, last-gasp screamers, superhuman individual performances, over 500 games, over 100 goals – many of them special – and the fourth Liverpool skipper to lift the European Cup. Job done.

我只想说：关键时刻的进球，最后时刻令人期待的一个人，超人般的个人表演，超过500次的出场，100多粒进球，当然还有更特别的，利物浦历史上第四位举起大耳朵杯的队长，相当不错。

Nanjing Lishui Meng wins Daomo Limited of the introduction of high-tech equipment and professional production machinery blades, mold equipment and hardware tools of medium-sized enterprises, is a professional team, the factory has more than 2,000 square meters of factory buildings , Scientific research, development, manufacture and sale in one, with the increasing market demand, our business has been a corresponding expansion of our products according to national standards and standards of production enterprises, strictly in accordance with the ISO9001 quality management system requirements , Complete the processing equipment, a reasonable manufacturing process, improve the detection means strong design capability, to provide high-quality products, the company specializing in the production of various models bending machines, Flap machine, mold and Chongjian, etc. Specifications of plastic spiral Qieli Ji hob, cutting plate machine blade, on paper at the next round Paper knife, the shape of the cardboard boxes with knives, knife type roll, spiral knives, steel rolling knife, thrown knives, plastic models with blades, Cardboard slotted knife-cutter, repair knife edge, high-alloy.

南京溧水猛胜刀模有限公司系引进高新设备与专业生产机械刀片、模具及五金工具设备的中型企业，是一个有专业人员组成的团队，工厂拥有2000多平米的厂房，集科研，开发，制造销售于一体，随着市场需求的不断增加，我们的业务也得到了相应的扩展，我们的产品均按国标及企业标准组织生产，严格按照ISO9001质量体系要求进行管理，齐全的加工设备，合理的制造工艺，完善的检测手段较强的设计能力，为客户提供高质量的产品，本公司专业生产各型号折弯机，折边机，冲剪机等模具及各种规格塑料螺旋切粒机滚刀，剪板机刀片，切纸分纸上下圆刀，各形号纸箱用刀具，横切辊刀，螺旋刀，轧钢刀，甩刀，各型号塑料用刀片，纸板开槽刀，分切刀，修边刀，高合金。

Every treatment combination has 4 replications.(1)The germination indexes of the former are higher than that of the latter; the germination of the former is stimulated by NaCl density of 0.3 %, while that of the later is restrained by all kinds of NaCl density.(2)With increasing of temperature and PEG indesity,the order of germination rate and vigor of Bromus stamineus and Bromus inermis is decreaced.(3)Under treatment of broadcast sowing and plant density of 549kermels·m~(-2), Bromus stamineus has the highest bud rate, fresh weight, seed yield, while treated with 25cm row spacing and 412kermels·m~(-2) plant densities, tillers, plant height, growth rate increased to the top;(4) Under treatment of broadcast sowing and plant density of 549kermels·m~(-2), its growth rate is took second place, while treated with 15cm row spacing and 824kermels·m~(-2) plant densities, it has the highest seed yield

3撒播时，引进种在播种密度为549粒·m~(-2)处理下单位面积出苗率最高，分蘖数最多，鲜重最大，单株最高，种子产量最大；生长速度在播种密度为412粒·m~(-2)处理下生长最快，密度为549粒·m~(-2)处理下稍次之，结合方差分析可以初步确定它在撒播时的播种密度为549粒·m~(-2)；(4)条播时，在行距为25cm、播种密度为412粒·m~(-2)处理下单位面积分蘖数最多，鲜重最大，单株最高，生长速度最快；种子产量在行距为15cm、播种密度为824粒·m~(-2)处理下最大，在行距为25cm、密度412粒·m~(-2)处理下次之；结合方差分析，就3种规格的行距来说，引进美国无芒雀麦条播时的行距为25cm，播种密度为412粒·m~(-2)；就这两种播种方式来说，应该选择撒播。

1,After transfected the mutated mtDNA of colorectal carcinoma,the mtDNA D-loop region of the transfected cells displays new mutation points.2,The external source pieces of the mutated mtDNA can integrate to nuclear genome after transfection.3,There's no differences in apoptosis between combinations after transfected the mutation of mtDNA in NIH3T3 and LST cells.4,The mutated mtDNA may affect the action mechanism of occurrence and development in colorectal carcinoma through affecting its mtDNA mutation or integrating exogenetic mtDNA to its nuclear which may cause the abnormal expression of oncogene or anti-oncogene.

（1）转染突变的大肠癌细胞mtDNA后转染细胞的mtDNA均可发生多处的突变位点。（2）通过转染后突变的外源性的mtDNA可以整合到核基因组内。（3）突变的mtDNA转染LST细胞及NIH3T3细胞后，不影响转染细胞的凋亡改变。（4）mtDNA的突变可能通过影响体细胞mtDNA的突变和通过外源性mtDNA在核内的整合从而影响癌基因或抑癌基因的表达异常，从而参与肿瘤的发生发展。线粒体DNA；D―环区；突变；质粒；pcDNA3.1；转染

Electron microscope observations showed that the liposome suspension was heterogeneous and the particle size ranged from 20 nm to 700 nm with a average diameter of 163 nm. The vesicles showed unilamellar and multilamellar structure which consisted of several concentric bilayers.

电镜观察表明，该脂质体多呈球形的单层或多层结构，脂质体粒径分布均匀，粒径范围20-700 nm，平均粒径163 nm，主要粒径分布位于50-200 nm(84.80%)。

The chromatin unfolding assay showed that ,like the wild-type transactivation domain, two variants that represent benign polymorphisms did not induce chromatin unfolding or only induced subtle change. Contrary to the behaviors of the wild type and two benign variants, four cancer-predisposing mutations in the transactivation domain superactivate the chromatin unfolding. The results suggest that the chromatin unfolding assay can aid in the characterization of deleterious mutations in the C-terminal transactivation domain of BRCA1 and may provide more reliable presymptomatic risk assessment.

对这些重组质粒的染色质伸展活性检测表明，野生型pwt和两种良性多态性突变体不具有染色质伸展活性或只有极微弱的染色质伸展活性，而其他4种乳腺癌易感突变体均具有过强的染色质伸展活性，提示利用染色质伸展技术可预测BRCA1转录激活区基因型与乳腺癌发生风险的表现型的关系。

The results showed that there was a more better effect of filmformation and absorbency to use poly 1788 as an adhesive and polyvinyl pyrrolidone(K:90) as a hydrophilic polymer. The silicon dioxide with particle size 0.3-1 μm as a inorganic pigment had a better absorbency. The polyethylene glycol modified with ethylenediamine had a more better characteristic of dye immobilization, absorbency and definition than the polyethylene glycol modified with diethylenetriamine, of which the polyethylene glycol of molecular weight of 400 modifiedwith ethylenediamine had more best performance.

研究结果表明：采用聚乙烯醇1788作黏合剂、聚乙烯吡咯烷酮(K值为90)作亲水性高分子可得到比较理想的成膜效果和吸墨效果；粒径分布范围在0.3-1 μm的二氧化硅作为无机颜料具有较好的吸墨性能；用乙二胺改性的聚乙二醇比用二乙三胺改性的聚乙二醇具有更好的固墨性、吸墨效果以及清晰度，其中乙二胺改性的分子量为400的聚乙二醇所得到的烷基多胺的效果为最好。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。