多粒的

In order to further study, with a new method of preparation of DNA fibers which has conquered the disadvantage of the way of squashing in liquefacient nitrogen,high-quality fibers were been gotten from maize which has large genome and contains numerous types of highly repeat DNA elements.

为了进一步分析，对现有的提核方法进行了改进，采用了新的方法制备DNA纤维，克服了液氮研磨法的不足，并在基因组比较大，重复序列较多的玉米上获得成功，同时用伸展DNA纤维的荧光原位杂交技术测定了端粒DNA在玉米染色体上的拷贝数。

In this materialistic world, more and more people of our generation have lost themselves and they can't successfully orient themselves.

在这个物欲横流的世界，年轻的一代里，越来越多的人迷失了自己，找不到正确的位置，我们都是大海里的一滴水，沙漠里的一粒沙，渺小得像尘埃。

Recombinant expression vector k14/c-myc-paav was constructed bearing the mouse c-Myc cDNA derived by the skin specific human keratin 14 promoter, then the linear vector was microinjected into the two pronucleus of the C57BL/6 fertilized eggs to produce transgenic mice.256 microinjected eggs were transferred, 6 of the foster mothers were pregnant and 44 adult mice were produced, two of them were positive transgenic mice confirmed by PCR and Southern blot analysis, transgenic rate was about 4%.

以CMV-PAAV载体为基础载体，以皮肤特异人K14（人角蛋白14）基因启动子替换原质粒中的CMV启动子后，在其多克隆位点区依次插入鼠c-Myc基因的cDNA序列和gfp基因的编码序列，构建了高效表达c-Myc基因的真核表达载体。通过双原核显微注射技术，将线性化的载体DNA注入到C57BL/6小鼠受精卵的两个原核中，然后将注射后状态良好的胚胎移植到代孕鼠的输卵管中，共移植了256枚受精卵，6只受体怀孕，最终获得44只成年小鼠。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

The company's alloy products used in high-quality tungsten carbide alloy powder as raw materials, the world's leading production technology, advanced selection of bilateral pressure XDZY-16A automatically Press, XDZL dumping rotating tube feeder system, XDPW spray drying system Tablets of equipment, XDYT vacuum sintering dewaxing one furnace, precision control of temperature and pressure control equipment for fully automated operation, with more than climate control and ensure stability in the production process.

本公司生产的合金制品系采用优质的碳化钨合金粉末为原材料，采用世界领先的生产工艺，选用先进的XDZY-16A双侧压自动压机，XDZL倾旋筒制料机，XDPW喷雾干燥制粒设备，XDYT真空烧结脱蜡一体炉，精密控制设备温度和压力控制为全自动化操作，带多气氛控制，保证了生产工艺过程稳定。

While the positive immunofluorescent staining of cenexin could only be seen in one of the centrioles, suggesting that the cenexin protein might be a special component of human matured sperm centrosome.

值得注意的是，利用抗Centrin多克隆抗体对18例男性不育病人精子的免疫荧光观察发现一些精子中Centrin蛋白的分布异常，主要表现为Centrin弥散，未显示正常大小的近端和远端中心粒。提示中心体蛋白异常是否为引致某些男性不育症的因素之一，值得进一步研究。

Experiments were performed in a gas-solid fluidized bed to determine the changes in the electrostatic charges with addition of various fine polyethylene particles,which were the same material as the coarse polyethylene particles,to better understand their role in influencing electros.

前人已经在流化床中的静电研究上做了很多工作[4-5]，但由于聚合物粉料粒径的多分散性，以及流化床中气固运动的复杂性和随机性，对气固流化床中静电产生和累积机理的研究相对较少。

The vacuum gas quenching process of ring die of pellet mill is analyzed and discussed based on selection of heat treatment equipment,setting of process parameters,comparison of gas quenching cooling medium and control of deformation amount and others.

对饲料制粒机环模的真空气淬工艺，从热处理设备的选用、工艺参数的设置、气淬冷却介质的比较及变形量的控制等多个方面进行了分析和讨论。

Our main products are the cork esries,such as cork granule,cup pad,cooking set pad,memo board, bottle stoppers,ball core of softball,baseball and badminton, and so on, with are always welcome in the market.Our scope of business is ceaselessly extended.At the same time,the other main products,sealing gasket series,which are famous for the rich assortment and good performance in leakproof and accurate size.They are well-assorted accessories which many engine companies of automabile and motorcycle confide in.

本厂生产的软木纸、软木粒、茶具垫、餐具垫、留言板、瓶塞、棒球、垒球、羽毛球芯等软木制品，深受国内外用户的欢迎，业务不断发展，同时生产内燃机密封垫片方面的产品，素以机型品种齐全，防漏密封性能好，几何尺寸精确而知名，深受国内多家大型的汽车、摩托车发动机制造公司的信任，并得以长期配套生产。

Observing through SEM and TEM, we could found that the diameter of zeolite particle is during 10~20nm, the diameter of straight chain diatomite is between 8μm and 20μm, and the diameter of discoid diatomite is between 15μm and 20μm. From the diagram of TGA and DGA, we could draw the conclusion that the adsorption capacity of diatomite could be increased by heating method under appropriate temperature. The results of FTIR analysis show that there are several libration between 1510 cm~(-1) and 1529 cm~(-1), and this result can sufficiently prove the adsorption of benzene hydrocarbons.

4对材料进行了表征，通过SEM和TEM可以观察到分子筛粒径大小在10～20nm之间，直链结构的硅藻土直径约为8～20μm，盘状硅藻土圆盘直径约为15～50μm；经过TGA和DGA证实了，对硅藻土进行适当温度的活化可以提高硅藻土的吸附效果；经过IR分析，可以发现在1510～1529cm~(-1)之间出现了多个振动峰，这证明了分子筛对苯系物的吸附效果。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。