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These patterns could result from REMI events, tandem integrations, or a combination of both.

这些现象可能是质粒多位点串联整合并且整合后发生重组的结果。

The multicopy gene saccharomycetes expressed recombinant plasmid pAOnsGH, the experiment shows, has very high expression activity to make the engineering saccharomycete product possess low production cost and high efficiency. Trial raising shows that feed with the said saccharomycetes added can raise the growth speed, disease resistance and output of the raised animals.

多拷贝基因酵母菌表达重组质粒pAOnsGH,经实验证明具有很高的表达活性,使本工程菌制品实现了生产成本低、效率高的效果,经多次水产养殖试验及养鸡试验,使用添加本酵母菌制品的饲料饲,喂养殖的动物可提高其生长速度和抗病害能力,提高单位养殖产量20%以上,增产的收入大于本制品成本投入的五倍以上。

We amied the BCP sequence and the preS2 gene sequence of HBV separately as the target region, according to HBV DNA sequence of Chinese strain, synthesized the set of specific primers, amplified the sequence by PCR method from the serum of patients with CHI, verified by restriction analysis, subcloned into pGEM Teasy vectors, employed polyacrylamide gel electrophoresis to display the deletion mutation and selected the clones with differential length to be sequenced.

本研究应用PCR技术及其他分子生物学技术,分别以HBV转录调控序列BCP以及表面蛋白编码序列pre S2为靶基因,以中国株HBV基因序列为依据,设计特异性多聚酶链反应引物,分别自43例、51例慢性HBV感染患者血清中扩增出目的片段,构建质粒Teasy-BCP和Teasy-pre S2,酶切鉴定后,采用聚丙烯酰胺凝胶电泳技术展示缺失突变,再经DNA测序以了解在慢性乙型肝炎患者体内的病毒变异情况。

An in vitro transcription recombinant plasmid 5T1d was obtained after subcloning the target gene of clone 5T1d,which contained mutant HCV 5 non-coding region cDNA,into a transcriptive vector pCDNA Ⅰ.

采用含HCV5非编码区(5NCR)缺失突变的重组质粒5T1d,将其目的基因亚克隆到体外转录载体pCDNAⅠ多克隆位点上,获得转录重组体5T1d。

The structure and principle of the new type of rubber balloon with water volumometer are introduced.

目前路基工程中,国内测定粗粒土及级配砂砾石等填料的干密度多采用灌水法。

The production technology for Mg and Mg alloy powders are: grinding milling,atomisation,melting and recycling,turning and chipping.

由于镁及镁合金的独特性能,其粉末及粉粒通常用球磨法、铣削法、雾化法生产,特别是铣削法用得最多。

While equal amount of MgSO4 was added ,bulks occurred in 5 hours.

微球的粒径在6h后趋于稳定,多分散性相应提高。

Cv. Xushu 18 and via immunogold electron-microscopy technique, showed that β-amylase visualized by gold particles was predominantly localized in plastids especially at periphery of starch granules, but the gold particles were scarcely found in other subcellular compartments, indicating that the enzyme is subcellularly compartmented in the same zone as its starch substrates.

本研究利用胶体金免疫电镜定位技术证明,甘薯块根生活细胞中的β-淀粉酶也是主要定位于质体内,围绕淀粉粒分布较多,其他亚细胞区域内β-淀粉酶分布很少,说明该酶主要分布于其功能区域。

And only a small amount of fluoridated toothpaste, the size of a green pea, should be used.

牙膏的用量只需一粒豌豆那么多。

With sales of more than 30 years to develop and produce feed mill and processing plants mature technology and professional equipment, production of Honor "star card" campaign grinder, mixer series, the series transmission equipment, the series of particles, and in middle-class 05,000 tons 40,000 tons to over-feed, concentrated feed, grain feed, grass feed pelleting complete sets of equipment, and other seven series over 80 products.

拥有30多年研制生产销售粉碎机及饲料加工成套设备的成熟技术和专业设备,荣誉生产&红星牌&系列粉碎机、系列混合机、系列输送设备、系列颗粒机、以及年单班产0.5万吨至4万吨以上级配合饲料、浓缩饲料、颗粒饲料、草饲料制粒成套设备等七大系列80多个产品。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。