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多种多样

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Fluorescence in situ hybridization was carried out to confirm the integration of HBV DNA into male pronucleus and its replication with cell division in embryonic development.(Reverse transcriptase polymerase chain reaction,RT-PCR) and immunofluoresence assay were performed to observe the expression of the HBV gene in two-cell stage.

材料与方法:成熟雄鼠麻醉后双侧睾丸注射经脂质体DOSPER包裹的HBV质粒,手术后雄鼠与超排雌鼠合笼交配,用荧光原位杂变(Fluorescence in situ hybridization,FISH)分别检测单细胞胚和二细胞胚间期核中HBV DNA的存在与复制,用逆转录聚含酶链反应(Reverse transcriptase-polymerase chain reaction,RT-PCR)和免疫荧光方法检测HBV基因在二细胞胚胎中的表达。

And the customer may buy its roller skate with just 1/3 of the traditional one.

这样,消费者就能一传统溜冰鞋1/3的价格买到它的产品。

After verification of the program, the mathematical models of the suspension bridges were studied by means of the program, as well as the determination of the main cable's shapes and its internal forces at different stages, the calculation of the cable's unstrained length and the analysis of the construction processes, of which some new approaches were presented in the mean time. Thus makes the structural analysis results of suspension bridges more reliable and in accordance with reality.

在用算例验证了程序分析结果正确性的基础上,应用该程序对悬索桥的数学模型、各阶段缆索几何形状及内力状态的确定以及缆索无应力长度的计算和施工过程分析进行了研究,提出了确定缆索在成桥状态下的几何形状及其内力状态以及缆索无应力长度计算的新方法,使悬索桥的结构分析结果更加符合实际情况。