多种免疫

Detecting each marker and cmparison the location of sequential tissuesection could determine the area loceation relationship of the markers, but it is difficulte to determine their correlation intra-cells exactly. No report of detecting virus genome and antigenes on a one section with triple staning techniques exists in the lit erature. in this report. by combining immunohistochemistry and in situ hybridization (with dioxigenin labelled HBV DNA probe), a triple staining technigce was established. With this technic, on one formalin-fixed and paraffin embeded section, HBV DNA, HBsAg and HBcAg were determinated one after the other.

各个细胞内病毒的复制和表达水平以及与肝细胞病变的关系，是乙型肝炎发病机理研究的一个重要方面：目前常用的在系列切片上对各标志物分别检测，比较观察的方法，一般只能大致了解各待检物在某一区域的定位关系，很难准确地了解它们在同一细胞内的关系：国内外尚未见有在同一张切片上三重标记染色检测病毒核酸及其多种抗原的报道：本文结合免疫组织化学ABC法及地高辛配基标记HBV DNA探针原位杂交，建立三重标记染色技术：在一张福尔马林固定石蜡包埋肝组织切片上先后检测，同时显示HBV DNA、HBcAg和HBsAg。

CD58, also was called lymphocyte function-associated antigen 3, is a surface glycoprotein expressed on many cell types, binds to the T lymphocyte surface glycoprotein CD2. This interaction is important in mediating T lymphocytes with target cells and antigen-presenting cells, and the T lymphocyte rosetting with erythrocytes. It plays an important role in mammal immune system.

CD58又称淋巴细胞功能相关抗原-3(Lymphocyte Function associated Antigen-3， LFA-3)，是存在于机体内多种细胞及血小板表面的一种糖蛋白，它可与CD2结合，从而介导T细胞与抗原递呈细胞等的黏附，此外，还可介导T细胞与绵羊红细胞形成Et花环，在机体免疫系统中具有重要作用。

To understand the infectivity by porcine endogenous retrovirus with porcine skin fibroblast cell in vitro and in vivo, porcine skin fibroblast cell established by our laboratory were co-cultured with neo/HEK293 cell for the infection of RERV in vitro, and were subcutaneously transplantated to SCID (severe combined immuno-deficiency) mice for the infection of PERV in vivo, laying the foundation for valuation of biologic safety of xenotrans-plantation. The event of neo/HEK293 cells infected by PERV occurred during co-culture of porcine skin fibroblast cells with neo/HEK293 cells, expanding the rang of the infection of porcine endogenous retrovirus. Afterpig cells transplantated subcutaneously in SCID mice, the microchimerism (78.57%) of pig cells occurred widel, and there was phenomena of integration of PERV provirus (85.71%) in several organs or tissues remote from the injected sites, indicating infection of PERV in SCID mice in vivo. yet, there is no evidence of active viral replication in analysis of PERV env RNA of these tissues or organs.

为了解猪皮肤成纤维细胞PERV在体外和体内的感染性，通过建立猪皮肤成纤维细胞系，将所建细胞系与人胚胎肾293细胞体外共培养，并移植于严重联合免疫缺陷鼠皮下进行猪皮肤成纤维细胞PERV的体外和体内感染性实验，结果表明，猪皮肤成纤维细胞与人胚胎肾细胞共培养过程中，猪内源性逆转录病霉感染人胚胎肾细胞，进一步证实和拓宽了猪细胞PERV感染人细胞的范畴；猪皮肤成纤维细胞移植SCID鼠皮下后，导致SCID鼠发生猪细胞微嵌合(78.57%)和PERV在体内感染(85.71%)并且波及远离移植部位的多种组织或器官，但是并未检测出SCID鼠组织中表达PERV env RNA。

To find the significance of oncogenes in the development of prostate cancer.

肿瘤的发生是多步骤的，涉及到多种癌基因和抑癌基因的改变［'］，我们采用免疫组化法对50例前列腺癌组织中bcl－2、P和c－erb－B。

Result:The effect of Niaoluqing on chronic urethritis was better than that of Sanjing Pian,the difference was significant.Niaoluqing could clear away the WBC,RBC,and pus cell in the urine,reduce the level of IgG,β2-MG,ALb in the urine,rapidly relieve the stimulus of urethra,improve the function of immunity.

结果：提示尿路清组显效率明显高于三金片组，对多种细菌导致的泌尿系感染作用良好，能很快清除尿中白细胞、红细胞、脓细胞，使尿中IgG、β2-MG、ALb指标下降，迅速解除泌尿系刺激症状，并改善免疫功能。

Now, research replaces the option of index in the various disease appraisement of curative effect to discuss , if with blood plasma 羟 hydrogen can appraise when ketone density is replacing index A flat kind ease pain medicine Kong Shi dose relieve pain effect , is taking ATT( aim at the antibody effect of tetanus toxin) as replacing index to appraise the immunity inhibition researchs of? single clone antibody 5c 8 of the CD 154 in monkey body to get better effect.

目前，已有研究就多种疾病疗效评价中替代指标的选择进行了探讨，如以血浆羟氢可待酮浓度作为替代指标评价阿片类镇痛药控释剂的止痛效果、以 ATT(针对破伤风毒素的抗体效应)作为替代指标评价猴体内 CD154的单克隆抗体5c 8的免疫抑制作用等研究都取得了较好的效果。

Study shows that strengthening body resistance and archaeus herbs have many effects,such as improving and adjusting immunity function,protecting bone marrow,improving haematogenesis function,raising digest and absorb function,improving substance metabolism,preventing gene mutation,inhibiting tumor cell proliferation,inducing tumor cell differentiation or apoptosis,resisting tumor invasion and metastasis,inhibiting formation of tumor vessel and activity of telomerase,etc.It's possibly the result of synergistic effect by multi-pathway and multi-target.

研究表明，扶正培本药物具有增强和调节机体免疫功能、保护骨髓及提高机体造血机能、增加消化吸收功能，改善物质代谢、阻止基因突变、抑制肿瘤细胞增殖、诱导分化、促使肿瘤细胞凋亡、抗肿瘤侵袭与转移、抑制肿瘤血管形成及肿瘤细胞端粒酶活性等多种作用，其发挥的扶正作用可能是多途径、多靶点联合协同作用的结果。

Cellular fibronectin is a kind of non-collagen glucoprotein. It is present mostly in periodontium, taking part in many physiological functions, such as cell junction, proliferation and differentiation, reparation and arrangement of epithelial tissue, immunoregulation of organism.

细胞纤连蛋白是一种具有生物学活性的非胶原糖蛋白，大量的存在于人的牙周组织中，排列规律，参与机体细胞的连接黏附、增殖分化和上皮组织的修复以及机体免疫调控等多种生理活动，是维持牙周附着的主要物质。

The result of immunohistochemical staining was different from that of conventional Congo red Staining.

βA4在脑组织中的表达并非Alzheimer病所特有，而是多种神经变性疾病的共同特征；βA4免疫组化法的阳性结果不同于传统的刚果红染色法。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。