多碱的

Bimolecular rate constants to DDVP, eserine, omethoate, methomyl and propoxur of resistant housefly were lower than that of the susceptible strain, which were 26.51, 7.80, 7.70,12.54 and 22.48 times, respectively, in comparison with the susceptible strain, indicating the enzyme was insensitive to these insecticides.

不同浓度的DDVP、毒扁豆碱和残杀威对家蝇AChE的抑制作用表明，随着DDVP和残杀威浓度升高，AChE的残存活性降低较多，抗性家蝇AChE的残存活性高于敏感家蝇，而随着毒扁豆碱浓度的增加，AChE残存活性降低较少，并且当毒扁豆碱浓度很低时，在一定范围内，敏感家蝇AChE的残存活性高于抗性家蝇。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Of alfalfa grass against as many as 65 kinds of products, including dicotyledonous weeds against the most serious, there are: Ma theophrasti, Xanthium, green salsa, salsa fin, Cephalanoplos vegetables, Salsola collina, flat hair, trionum wild, East City, quinoa, small Chenopodium, Portulaca oleracea, Amaranthus retroflexus, Capsella bursa-pastoris, iron amaranth, playing a bowl of flowers, vegetables brachyotus Chicory; monocotyledons mainly Setaria grass, barnyard grass, Chloris virgata, reed, Dichotomanthes grass, Digitaria Descurainia sophia, and so on.

对苜蓿有危害的杂草多达65个种，其中以双子叶杂草危害最为严重，主要有：苘麻、苍耳、灰绿碱蓬、翅碱蓬、刺儿菜、猪毛菜、扁蓄、野西瓜苗、东亚市藜、小藜、马齿苋、反枝苋、荠菜、铁苋菜、打碗花、苣荬菜；单子叶杂草主要有狗尾草、稗草、虎尾草、芦苇、牛筋草、播娘蒿和马唐等。

After discussion of the relationship among idioblastic cells,tolerance to abiotic stress and betaine,the author thought the reason of transgenic plants having an increased number of idioblastic cells was that BADH was introduced and much betaine might be synthesized.

通过对异形细胞、植物抗逆性、甜菜碱三者之间的关系讨论，认为转基因植株异形细胞数量多是由于BADH基因的导入可能合成了较多甜菜碱。

Both Stemona sessilifolia and Stemona japonica had more protostemonine which was 0.22%～0.36 percent of all. No protostemonine was in Stemona tuberose.

蔓生、直立百部均含有较多的原百部碱，所取样品中含量为0.22%～0.36％；对叶百部中并不含有原百部碱。

In order to utilize lignin effectively, base on the situation in China, the study was carried on systematically, centering around the topic of two step sulfomethylation of the soda lignin of wheat straw (the first step is methylolation, the second is sulfonation) and its application. The main purpose is to develop a heterogeneous catalytic system which not only catalyzes but also homogenizes the lignin molecule in the reaction.

为真正高效利用碱木素，木论文根据我国实情，紧紧围绕着麦草碱木素两步磺甲基化（第一步为羟甲基化，第二步为磺化）及其应用这一主题，以开发出在反应过程中既可催化反应又能使木素分子均一化的多相催化体系为主要目标，系统地展开了研究。

Based on simulation principles of two-phase flow,a test rig has been set up for studying multi-phase flow,during flue gas desulfurization ,in topping alkaline atomizers.

根据两相流相似模化原理建立了前置碱雾发生器烟气脱硫颗粒图像测速技术多相流动实验台，运用颗粒图像测速技术对前置碱雾发生器内气液固三相流动进行测量，得到不同工况条件下喷雾射流与固体颗粒的流动结构和流场信息。

For example, significant correlation in Zunyi prefecture was found between smoking total scores and total nitrogen, nitrogen to nicotine ratio and nicotine, and between offensive odor and total nitrogen and chloride.

在省级尺度，烟叶化学成分与烟叶品质的关系更为复杂，需用更多的化学成分（碳水化合物、含氮化合物及矿质养分）才能综合反映烟叶的品质，如吸食总分与总氮、糖碱比、氮碱比、磷、镁显著相关，杂气与总氮、糖碱比、氮碱比、磷、镁和还原糖显著相关。

At the prefecture scale, the relationship became complicated between chemical components and leaf quality, and more chemical components were needed to represent leaf quality. For example, significant correlation in Zunyi prefecture was found between smoking total scores with total nitrogen, nitrogen to nicotine ratio and nicotine, and between offensive odor with total nitrogen and chloride.

在省级尺度，烟叶化学成分与烟叶品质的关系更为复杂，需用更多的化学成分(碳水化合物、含氮化合物及矿质养分)才能综合反映烟叶的品质，如吸食总分与总氮、糖碱比、氮碱比、磷、镁显著相关，杂气与总氮、糖碱比、氮碱比、磷、镁和还原糖显著相关。

1 Filling amount: 30 tons of bamboo counted with liquor filled tank and circularly press the bamboo flakes for 3-4 minutes during boiling,(the data results from repeated experiments), if the filling amount is too less, the hardness is low, too much alkali remnant, and the pulp yield will be low; if the filling amount is too much, the cooking liquor can not be dried completed, and the circulation amount is too less, the electric current of circulating pump is too low, it will be difficult to cook, and there will be too much pulp knots with high hardness and less alkali remnant which may lead to unripe pulp.

3.1.1装锅量：竹子30吨是以液浸装锅和开中循环压竹片3——4分钟为计，（数据是根据多次实验得出的结果）如装锅量太小，硬度低，残碱大，浆得率低，装锅量太大蒸煮液无法完全送完，循环量小，循环泵电流小，蒸煮困难，浆节子多、硬度高、残碱小，有可能造成生浆。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。