多碱的

Based on the relationship between the structure and function, a lot of research have been performed on predicting base-pair which is indispensable to the RNA three-dimensional strcutrue. The tools and methods in Combinatorics and Statistics will play important roles in studying linear sequences of biomolocular units . Similarly, we can present the geometric representation of biological sequences and structures. So the genometric topology and group theory are important also.

根据RNA的结构与功能的关系，在预测碱基配对的类型方面进行了相当多的研究，这也是确定RNA三维构象的必不可少的一部分，组合学和统计学的工具和方法在研究生物序列和生物结构上起到了很大的作用，同样我们可以用几何方法来表示生物序列和二级结构，几何拓扑和群论也起到了很大的作用。

The results are as follows:Defatted wheat germ was micronized useing both hammer mill and colloid mill to increase the extraction rate of protein, followed by alkali extraction and ultrafiltration, respectively. A quadratic mathematical model was statistically constructed by using of Box-Behnken central composite design and response surface methodology. Under the optimized processing parameters of 23μm particle sizes, pH 9.5 and 51℃, a maximum extraction rate, 68.6%, was achieved, which is 30.63% higher than that of non- micronized wheat germ (passing through 80 mesh sieve).

首先采用锤式粉碎与胶体粉碎相结合的方法对脱脂麦胚进行微细化处理，然后利用碱提、超滤浓缩法提取制备麦胚蛋白，并通过Box-Behnken中心组合设计及响应面分析建立预测麦胚蛋白提取率的二次多项数学模型，优化蛋白质的提取工艺，在麦胚粒度为23μm、pH值9.5、提取温度51℃的条件下，经微细化处理的蛋白提取率为68.6％，比未经微细化处理的(过80目筛)提高30.63％。2。

Some approved aneugens Trip terygiuwlIypogaucum (evelHutch, colchicine, thiabendazole, a tropine sulfate,d-ubocurarine was added into the reactive system respectively and the changes of their absorption values were taken down during different periods under the conditions of 37 0C and 350nm .

本研究通过多步聚合和解聚合反应从猪脑中分离纯化微管蛋白，建立微管蛋白的体外聚合和解聚合反应平衡体系，并将已证实的非整倍体诱发剂（昆明山海棠根部水抽提物、2-（4′-噻唑）苯丙咪唑、硫酸阿托品、d-筒箭毒碱）分别加入此反应平衡体系中，于37℃、350nm条件下，记录此体系不同时期的吸光值变化，与阳性对照物和溶剂对照处理平衡体系后的吸光值在相同时间点作比较，以此评估这几类非整倍体诱发剂对离体条件下的微管蛋白聚合的影响，寻求这几类非整倍体诱发剂是否可通过抑制微管蛋白聚合作用途径而诱发非整倍体。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

Process for the preparation of phosphatidylserine of formula wherein R and R independently represent a saturated, mono-unsaturated or polyunsaturated acyl C10-C30, X=OH or OM where M=alkaline or alkaline earth metal, ammonium, alkylammonium including the transphosphatidylation reaction between phosphatidylcholine of the general formula wherein R and R and X have the above specified meanings, R=CH2-CH2- NH2 o CH2-CH2-N(CH3)3 and Serine in D, L or racemic form catalysed by the phospholipase D enzyme, characterised in that said reaction is carried out in a hydroalcoholic medium containing an aliphatic alcohol and in the presence of bivalent metal oxide.

本发明涉及制备式CH 2 OR 1 CHOR 2 CH 2 O-P-OCH 2 -CH(NH 2 )-COOHX的磷脂酰丝氨酸的方法，其中R 1 和R 2 独立地表示饱和的、单不饱和的和/或多不饱和的酰基C 10 -C 30 ，X＝OH或OM，其中M＝碱或碱土金属、铵、烷基铵，该方法包括通式CH 2 OR 1 CHOR 2 CH 2 O-P-OR 3 X的磷脂酰胆碱其中R 1 和R 2 以及X具有上述特别的含义，R 3 ＝CH 2 -CH 2 -NH 2 或CH 2 -CH 2 -N (CH 3 3 与D、L或外消旋形式的丝氨酸之间的转磷脂酰化反应，该反应是通过磷脂酶D酶催化的，其特征在于所述的反应是在包含脂族醇的含水醇介质中并且在二价金属氧化物的存在下进行的。

Proline was much more accumulated in the fleshy leaves of K.foliatum with increased salt concentration concided with the expression pattern of K.foliatum P5CS, suggesting that proline and betain as two effective osmolytes had important functions for Kalidium foliatum during stress.

检测的各基因均受盐的诱导。P5CS和KfBADH基因的表达随着盐浓度的升高而表达逐渐增强，而脯氨酸的含量也随着盐胁迫程度的增强而在盐爪爪肉质化叶内积累更多，说明脯氨酸和甜菜碱作为重要的渗调物质，在植物逆境胁迫中可能发挥着重要的作用。

Compared the equivalent ratio relationship of granule for clincal prescription and clincal decoction by determination of evodiamine, rutaecarpine, evodine, total alkaloids and dried extract ratio as markers, ten batches reference decoctions were prepared according to clinical usage as evaluation standards, common-use processed fructus evodiae products, such as salt-processed fructus evodiae, liquorice-proccesed fructus evodiae, as researching objects, and finally validated by pharmacological trials.

以吴茱萸胺、吴茱萸次碱、吴茱萸内酯、吴茱萸总生物碱、出膏率为评价指标，以临床煎剂的煎煮方法制备的10批对照汤剂的平均值为临床煎剂的评价标准，以多个临床常用的炮制品为研究对象，比较配方颗粒和临床煎剂间的等效比例关系，并以药效指标进一步验证等效比的正确性。

In order to modify the recycled fiber, this study was to investigate the variations of the fiber and paper properties after paper recycling, reusing, and re-processing. Three methods for upgrading the recycled fiber, included alkali socking, enzyme treatment, and adding carboxymethyl cellulose to enhance the fiber strength, extend the fiber service life, and reduce the use of the virgin pulp.

中文摘要本论文探讨纸张的回收-利用-再生过程中纤维性质及纸张性质的变化，以做为后续纤维及纸张性质的改良，并分别继续使用碱处理、酵素处理及添加CMC的方法将再生后纤维改质，以期达到增强或改善纸张性质，进而提高再生纤维的使用范围、延长纤维的使用时间绵续纤维之生命周期与减少利用原生纸浆之多项目的。

In the case of decorative zinc electroplatings, a further enhancement of the appearance of such substrates in addition to the corrosion resistance imparted is achieved by the passivate film which ranges from a clear bright to a light blue bright appearance simulating that of a chromium deposit or alternatively, a clear light-yellow appearance simulating that obtained by use of prior art hexavalent chromium solutions.

可选择的治疗方法，可进一步遏制卤化物包括氟离子、氯化物和溴化物离子进一步增加了硬度钝化膜以及一个或多个兼容为实现高效润湿剂接触基板对待。现在的发明特别地可适用但是不限制在碱而产生酸非氰化物的锌的治疗使电解沉积传授对被对待的基体改良的腐蚀抵抗和装饰的外表。

It can be concluded by 16S rDNA sequences that moderately halophilic bacteria are novel species of genus Marinococcus and genus Lentibacillus. Further study for proving this novel observation is still in progress. At the same time, halophilic archaea strains, broken in distilled water and haloalkaliphilic archaea strains, grown in medium of pH9.5 were isolated from these samples and sequencing of the 16S rDNA sequences are being conducted taking other polyphasic taxonomy identification.

中度嗜盐菌通过对16SrDNA序列得测定，初步推断可能有Marinococcus属和Lentibacillus属的新种，后续鉴定工作还在进行中；同时，分离到到能在蒸馏水中破壁的嗜盐古菌菌株，另外分离到在pH9.5培养基中生长的嗜盐嗜碱古菌菌株，正在对其进行16S rDNA序列的测定和其它多项分类鉴定工作。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。