多液凝胶

Objective To evaluate the safety and effectiveness of protein-free calf blood extract eye gel for repairing corneal epithelial defect by multiple centers, double mask and randomized clinical study with parrel contrast of bFGF eye gel as positive control.

目的 以碱性成纤维细胞生长因子滴眼液为阳性对照药物，通过随机、双盲的多中心平行对照临床试验，评价小牛血去蛋白提取物眼凝胶治疗角膜上皮缺损的疗效和安全性。

Cytotoxicity experiment: In the leaching liquid of γ- poly-glutamic acid hydrogel, the L929 cells were with good shape and grew adherently. MTT experiment demonstrated that the toxicity of the leaching liquid of γ-poly-glutamic acid hydrogel was 0 or 1 degree.

细胞毒性实验结果：L929细胞在γ-多聚谷氨酸水凝胶浸提液中形态正常；MTT检测表明γ-多聚谷氨酸水凝胶浸提液组毒性为0级或1级。

The screening was carried out by single strand conformational polymorphorsim : the PCR product precipitation was dissolved in 20μl denaturing and loading buffer (95%+20mMEDTA+ 0.05% bromophenol blue+0.05% xylene cyanol), heat-denatured in 95 ℃ to two single DNA strands.

通过单链构象多态性分析筛查RDS基因突变；沉淀回收的PCR产物以20μl变性上样液（95%甲酰胺+20mMEDTA+0.05%溴酚蓝+0.05%二甲苯蓝）溶解后热变性为两条DNA单链后，上样于8%非变性聚丙烯酰胺凝胶（含5%甘油），700伏电压、4℃电泳16小时。

Trans-Blot Cells and Systems 170-3825 Trans-Blot Cell With Wire Electrodes and PowerPac HC Power Supply, 100120/220240 V, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm), power supply, power cord, instructions 170-3850 Trans-Blot System With Plate Electrodes and PowerPac HC Power Supply, 100120/220240 V 170-3853 Trans-Blot System With Plate Electrodes, Super Cooling Coil, and PowerPac HC Power Supply, 100120/220240 V, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm), power supply, power cord, instructions 170-3910 Trans-Blot Cell With Wire Electrodes, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) 170-3939 Trans-Blot Cell With Plate Electrodes and Super Cooling Coil, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) 170-3946 Trans-Blot Cell With Plate Electrodes, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) Trans-Blot Cell Accessories 170-3912 Super Cooling Coil, required for all high-intensity transfers 170-3913 Gel Holder Cassette, includes 2 fiber pads 170-3914 Fiber Pads, 15.5 x 20.5 cm, 6 170-3920 Trans-Blot Standard Wire Electrode Card, cathode 170-3921 Trans-Blot Standard Wire Electrode Card, anode 170-3922 Trans-Blot Cell Buffer Tank 170-3923 Trans-Blot Cell Lid With Power Cables 170-3943 Trans-Blot Platinum Anode Plate Electrode 170-3944 Trans-Blot Stainless-Steel Cathode Plate Electrode 170-3945 Trans-Blot Plate Electrode Pair, platinum anode and stainless-steel cathode 16 规格:说明: Trans-Blot Plus

电泳转印槽组件 1。缓冲液槽及带有电缆的盖 2。凝胶支架转印夹 3。纤维衬垫 4。电极丝 5。电极板 6。特级冷却芯 Trans-Blot 转印槽是功能灵活的转印设备，可理想地用于多种转印应用。Trans-Blot 转印槽特点包括：*能进行多胶转印，可容纳3 块PROTEAN I xi 凝胶、6块Criterion 凝胶、12块Mini-PROTEAN 3 或Ready Gel 预制胶*多组参数灵活可设，可调节的电压设置（从30 V 的过夜转印到200 V 的1 小时快速实验）*电极间距设置为8 cm 用于标准印迹杂交，或设置为4cm 用于高强度印迹杂交*可选择板式电极：涂有铂金的钛作为正极，不锈钢为负极，能提供高强度电场和比其它电极更高的电流密度。或选择较经济的铂金电极丝*通过特级冷却芯和水循环仪来调节温度―是天然酶（4°C）或高强度转印的理想选择，随着转印时间增加（多达24 小时），不会引起缓冲液耗竭（在高强度转印中必须使用冷却芯，也推荐用于所有板式电极的应用）*带铰链的凝胶支架转印夹能避免滑动，确保凝胶与印迹膜间的紧密接触；每个转印夹都有颜色标记以保证在转印槽中的正确定位 Trans-Blot 转印槽的锁闭凝胶支架转印夹系统。转印夹（1）支撑凝胶（2）印迹膜（3）两侧有纤维衬垫和滤纸（4）确保凝胶三明治内的完全接触。凝胶夹垂直插入缓冲液槽中（5）。

Trans-Blot Plus Cell 170-3990 Trans-Blot Plus Cell With Plate Electrodes and Super Cooling Coil, includes 3 gel holder cassettes, buffer tank, lid with power cables, 6 fiber pads, 1 pack blot absorbent filter paper (26.5 x 28 cm, 30 sheets), roller, stirbar 170-3991 Trans-Blot Plus Cell and PowerPac HC Power Supply Accessories 170-3994 Trans-Blot Plus Gel/Cassette Assembly Tray 170-3995 Fiber Pads, 27 x 28.5 cm, 2 170-3996 Blot Absorbent Filter Paper, 26.5 x 28 cm, 60 sheets 170-3997 Stirbar 170-3998 Trans-Blot Plus Roller, 6 wide 170-39 Trans-Blot Plus Gel Holder Cassette With Clamps 170-4990 Trans-Blot Plus Super Cooling Coil 170-4991 Trans-Blot Plus Platinum Anode Plate Electrode 170-4992 Trans-Blot Plus Stainless-Steel Cathode Plate Electrode 170-4995 Trans-Blot Plus Cell Buffer Tank 170-4996 Trans-Blot Plus Cell Lid With Cables 170-4997 Gel Holder Cassette Clamps, for Trans-Blot Plus cell, set of 3 9 规格:说明:运用Mini-PROTEAN II 多道筛选仪,可以快速有效地筛选 40 种不同的抗体或血清,无需把western

转印条件可调，能在很大的分子量跨度上获得最佳转印效果*耐用的板式电极能产生强大而均一的电场，而且电极在槽内的位置灵活可换；不论运行1、2 或3 个转印夹，电极间距可调节到最小以获得最大的场强和转印效率*凝胶支架转印夹保证整个凝胶与印迹膜表面的均衡接触*转印夹的铰链设计能防止凝胶滑动，便于转印夹组装*颜色标记的转印夹及电极板确保在转印槽内的正确定向*特级冷却芯和冷却水循环器进行温度调节―可理想地用于天然酶或高强度转印，或用于延长转印时间时减少缓冲液的损耗*可选择的组装盘是凝胶三明治和转印夹组装的理想选择 Trans-Blot Plus 转印槽能从单向和双向大格式凝胶上均一、高效地转移蛋白―配合使用新推出的 PowerPac HC 电源，多数蛋白都可在1530 分钟内完成转印。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。