多液

We optimized reaction system for labelling-antibody in which the optimal amount of the purified anti-Stx2B IgG conjugated with colloidal gold beads was 60μg/mL, the purified antibody for E.coli O157 was 57μg/mL, the optimal pH was 8.2, the size of colloidal gold partical was 20nm, the optimization of stabilizing agent was BSA, the optimization buffer was boracic acid buffer with pH 8.2, the optimization of preserving fluid and eluant was boracic acid buffer with pH 8.2 including 5mM NaCl-1%BSA, confining liquid for NC membrane was 0.01% PBST with 3%BSA, the amount of polyclonal antibody against E.coli O157 and Stx2 conjugated with colloidal gold beads for conjugate pad was 3μg respectively, the amount of anti-Stx monoclonal antibody for test line was 0.1μg and 1μg for E.coli O157, the amount of goat anti rabbit IgG for control line of both GICA were 1μg.

测定了胶体金颗粒的最优化反应体系：胶体金颗粒的大小为20nm；抗体与胶体金溶液结合的最佳pH约为8.2；最佳蛋白结合量分别为抗大肠杆菌O157 IgG为57μg/mL，抗重组Stx2B IgG为60μg/mL；最佳稳定剂为BSA；最佳缓冲液为pH8.2硼酸溶液；最佳金标保存液和洗涤液为5mM NaCl-1%BSA的pH 8.2的硼酸缓冲液；NC膜的封闭液为3%BSA的0.01mol/L PBST；Stx2试纸条和大肠杆菌O157试纸条的质控线上的羊抗兔IgG的多克隆抗体最佳点样量均为1μg，其检测线的抗Stx2的单克隆抗体的点样量为0.1μg、抗大肠杆菌O157的单克隆抗体的点样量为1μg，结合垫上的金标抗大肠杆菌O157和重组Stx2B多克隆抗体点样量均为3μg。

The comparisons of biochemistry between OP and OR rats The differences between OP and OR rats included not only body weight, but lipids metabolism and insulin sensitivity as well, characterized with insulin resistance, increasing in serum free fatty acids and ketone body, and hepatic TC and TG in OP rats. However, no significant differences were observed in serum TG, TC, LDL, HDL and fasting glucose between OP and OR rats.⑶Comparisons of metabolites in serum, urine and liver tissue between OP and OR rats①There were significant differences in amino acids concentration between OP and OR rats,especially in liver tissue, such as high concentrations in ketogenic and glucogenic amino acids in OP rats, suggesting differences in amino acids metabolism;② The different metabolites between OP and OR rats included increasing of various saturated fatty acids and decreasing of polyunsaturated fatty acids in OP rats;③The urinary metabolites analysis indicated that different structure or metabolism of gut microflora might exist between the two phenotypes, which probably influenced the regulation of body weight gain;④The end-products of catecholamines in urine and intermediates of krebs cycle in serum in OP rats were all up-regulated, suggesting that the activity of sympatheic nervous system and energy metabolism was higher in OP rats than OR rats.

胰岛素耐受实验和胰岛素敏感指数表明OP动物的胰岛素敏感性较OR动物下降，而OP大鼠血清中游离脂肪酸、酮体、肝脏总胆固醇和甘油三酯水平显著升高；但是，OP与OR大鼠血清中总胆固醇、甘油三酯、低密度脂蛋白、高密度脂蛋白和空腹血糖等的水平并无显著性差异；⑶肥胖易感与肥胖抵抗大鼠血清、尿液和肝脏组织提取物中代谢物的比较研究表明：①OP与OR大鼠的血清、尿液和肝组织提取物中多种氨基酸的含量存在显著差异，并以肝组织中的差异氨基酸数量为最多，包括各种生酮和生糖氨基酸水平在OP组的升高，说明氨基酸代谢的差异是两种体重表型大鼠之间存在的重要差异特征之一；②OP与OR动物肝脏和血清差异代谢物中包含多种饱和长链脂肪酸的升高如十四烷酸、十六烷酸、硬脂酸等和多不饱和脂肪酸的下降如亚油酸和花生四烯酸，说明两种体重表型动物的肝脏脂肪酸代谢存在明显差异；③长期高脂饮食喂养后，动物的尿液代谢物分析表明OP与OR动物体内的肠道菌群结构存在差异，这些菌群上的差别可能在动物体重增长的调节上产生影响；④与OR动物相比，OP动物尿液代谢物中儿茶酚胺类递质的代谢终产物如高香草酸、扁桃酸和4-羟基苯乙酸明显升高。

UDZ Series liquid level indicator of flap disassembly fast and convenient, the installation position can be arbitrary choice, free to change the direction of observation, thus instrumented great visibility; UDZ series of cost-effective liquid level was significantly higher than that used multiple sets of glass plate liquid level of cost performance; UDZ series of liquid level indicator, the sender's device, as well as the liquid level with the main transmission medium within the complete separation of the measured structural design, so that level gauge in the job has pressure (up to 320KG), toxic and inflammable conditions, with absolute safety and reliability; UDZ-3 type uses innovative design, improve the sensitivity of the sensor, and its near-zero drift FarEasTone signals and long-term stability so that it has always been at the scene consistent level instructions; sensors and the main body has a certain interval, which can be solved effectively under high-temperature liquids (up to 480 ) remote detection and control; antifrost type UDZ Series Level Gauge, are I plant the patented product (Patent No.: ZL00221022 3), guarantees that the use of cryogenic liquid level gauge is not freezing (such as liquid CO2), the effective guarantee of the level of a normal job.

UDZ系列液位计的翻板指示器拆装快捷方便，安装方位可任意选择，观察方向可随意改动，因而仪表指示的可视度很大；UDZ系列液位计的性能价格比明显高于采用多组玻璃板式液位计的性能价格比；UDZ系列液位计的指示器，发讯器、以及液位变送均与主体内被测介质完全隔离的结构设计，使液位计工作在有压（可达320KG）、有毒和易燃的工况下，具有绝对的安全性和可靠性；UDZ-3型采用了新颖设计，提高了传感器的灵敏度，其远传信号近乎零温漂和长期稳定性使其始终于现场液位指示保持一致；传感器和主体有一定的间隔，因而能有效地解决高温液体（可达480℃）远距离检测和控制；防霜型UDZ系列液位计，是我厂的专利产品（专利号：ZL00221022·3），保证了在低温液体上使用液位计不结冰（如液体CO2），有效的保证了液位计的正常工作。

METHODS: Rat bilateral olfactory bulbs and olfactory mucosa at 1/3 nasal septum were obtained, sliced, digested in trypsin, and made into monoplast suspension. At 1×109/L, cells were incubated in uncoated 25 cm2 culture flask. At 18-20 hours, cell suspension was moved into another uncoated 25 cm2 culture flask (the first differential adhesion). At 24 hours, cell suspension was moved into a poly-D-lysine-coated 25 cm2 culture flask or poly-D-lysine-coated 6-well culture plate (the second differential adhesion). At 48 hours, parenchyma cells were removed after a half of medium was changed.

完整取大鼠双侧嗅球及剪取鼻中隔后1/3嗅黏膜，剪碎后胰酶消化，制成单细胞悬液，按1×109 L-1密度接种于未包被的25 cm2玻璃培养瓶中，18~20 h后将细胞悬液转移至另一未包被的25 cm 2玻璃培养瓶中（第1次差速贴壁），24 h后再将细胞悬液转移至经多聚右旋赖氨酸包被的25 cm2塑料培养瓶或经多聚右旋赖氨酸包被的6孔培养板中（第2次差速贴壁），接种培养48 h后半量换液以去除杂细胞。

Trans-Blot Cells and Systems 170-3825 Trans-Blot Cell With Wire Electrodes and PowerPac HC Power Supply, 100120/220240 V, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm), power supply, power cord, instructions 170-3850 Trans-Blot System With Plate Electrodes and PowerPac HC Power Supply, 100120/220240 V 170-3853 Trans-Blot System With Plate Electrodes, Super Cooling Coil, and PowerPac HC Power Supply, 100120/220240 V, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm), power supply, power cord, instructions 170-3910 Trans-Blot Cell With Wire Electrodes, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) 170-3939 Trans-Blot Cell With Plate Electrodes and Super Cooling Coil, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) 170-3946 Trans-Blot Cell With Plate Electrodes, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) Trans-Blot Cell Accessories 170-3912 Super Cooling Coil, required for all high-intensity transfers 170-3913 Gel Holder Cassette, includes 2 fiber pads 170-3914 Fiber Pads, 15.5 x 20.5 cm, 6 170-3920 Trans-Blot Standard Wire Electrode Card, cathode 170-3921 Trans-Blot Standard Wire Electrode Card, anode 170-3922 Trans-Blot Cell Buffer Tank 170-3923 Trans-Blot Cell Lid With Power Cables 170-3943 Trans-Blot Platinum Anode Plate Electrode 170-3944 Trans-Blot Stainless-Steel Cathode Plate Electrode 170-3945 Trans-Blot Plate Electrode Pair, platinum anode and stainless-steel cathode 16 规格:说明: Trans-Blot Plus

电泳转印槽组件 1。缓冲液槽及带有电缆的盖 2。凝胶支架转印夹 3。纤维衬垫 4。电极丝 5。电极板 6。特级冷却芯 Trans-Blot 转印槽是功能灵活的转印设备，可理想地用于多种转印应用。Trans-Blot 转印槽特点包括：*能进行多胶转印，可容纳3 块PROTEAN I xi 凝胶、6块Criterion 凝胶、12块Mini-PROTEAN 3 或Ready Gel 预制胶*多组参数灵活可设，可调节的电压设置（从30 V 的过夜转印到200 V 的1 小时快速实验）*电极间距设置为8 cm 用于标准印迹杂交，或设置为4cm 用于高强度印迹杂交*可选择板式电极：涂有铂金的钛作为正极，不锈钢为负极，能提供高强度电场和比其它电极更高的电流密度。或选择较经济的铂金电极丝*通过特级冷却芯和水循环仪来调节温度―是天然酶（4°C）或高强度转印的理想选择，随着转印时间增加（多达24 小时），不会引起缓冲液耗竭（在高强度转印中必须使用冷却芯，也推荐用于所有板式电极的应用）*带铰链的凝胶支架转印夹能避免滑动，确保凝胶与印迹膜间的紧密接触；每个转印夹都有颜色标记以保证在转印槽中的正确定位 Trans-Blot 转印槽的锁闭凝胶支架转印夹系统。转印夹（1）支撑凝胶（2）印迹膜（3）两侧有纤维衬垫和滤纸（4）确保凝胶三明治内的完全接触。凝胶夹垂直插入缓冲液槽中（5）。

Most pleural effusion aroused by heart failure is bilateral, the quantity of effusion is always small or middle, the effusion is always exuding, the colour of the pleural effusion is always yellow, but the transudate or sanguinolent effusion also can be aroused by heart failure.

心力衰竭引起的胸腔积液多为双侧；其积液量以少量多见；性质多为漏出液，颜色多为黄色，但血性及渗出性积液亦不能除外心源性胸腔积液。胸腔积液；心力衰竭；渗出液；漏出液

Compare the data structures of pressure fluctuation signals and AE signals both sampled in gas-solid fluidized bed, and the difference of data structures among AE signals sampled in gas-solid fluidized bed, pneumatic transport tube, slurry bubble column, liquid-solid loop reactor, and liquid-solid stirred tank. Based on the compare, the corresponding relation between signal structure and physical multi-scales was established.

通过对气固流化床的声发射信号与压力脉动信号的结构比较，以及气固流化床、气力输送、气液固淤浆鼓泡床、液固环管及液固搅拌釜中声发射信号多尺度结构差异比较，建立了多相流体系随机信号多尺度结构与物理多尺度的对应关系，认为在有固体颗粒存在的多相流体系，微尺度信号代表了颗粒相对于主体流动的运动状态；介尺度代表主体流相对于系统的运动或者不同主体流之间的相互运动；宏尺度则反映整个系统状态随时间的变化。3。

The company has independently developed, development, production of the products have six series, more than 400 varieties, including: wire and cable series: high-temperature compensation wire, control cables, fire-retardant cables, anti-corrosion cable, power cable, 6 ~ 110KV crosslinked power cables, computer cables, silicon rubber cables, high temperature cables, fire-resistant cables, low smoke low halogen, low smoke zero halogen cables, such as the main cable tray; Temperature Instrument Series: ITS900 in line with the International Temperature Scale and IEC standards A, B-grade Thermocouple , Explosion-proof Temperature Transmitter integration, intelligent all-input temperature transmitter; Pressure / Differential Pressure Meter Series: general pressure gauges, precision pressure gauges, pressure / differential pressure switch, the general stress / Differential Pressure Transmitter, 1151 , 3051 smart capacitive pressure / differential pressure transmitter; Level Instrument Series: glass / glass level gauge, quartz glass tube liquid level, the electric-contact level gauge, magnetic liquid level transmitter, magnetic liquid level switch, input-type hydrostatic liquid level transmitter, liquid level transmitter float electricity, etc.; Flow Meters Series: All English Intelligent Vortex Flowmeter, Turbine Flowmeter; Display Instrument Series: Intelligent Control LED digital display device, Intelligent LCD screen controlled device, Guangzhu instructions figures, such as conditioning Alarm.

公司自主研制、开发、生产的产品主要有六大系列、400多个品种，其中有：电线电缆系列：高温补偿导线、控制电缆、阻燃电缆、防腐电缆、电力电缆、6～110KV交联电力电缆、计算机电缆、硅橡胶电缆、高温电缆、耐火电缆、低烟低卤、低烟无卤电缆主电缆桥架等；温度仪表系列：符合ITS900国际温标和IEC标准的A、B级热电偶，防爆型一体化温度变送器、智能全输入温度变送器；压力/差压仪表系列：一般压力表、精密压力表、压力/差压开关、普通压力/差压变送器，1151、3051智能电容式压力/差压变送器；液位仪表系列：玻璃管/玻璃板液位计、石英玻璃管液位计、电接点液位计、磁性液位变送器、磁性液位开关、投入式静压液位变送器、电浮筒液位变送器等；流量仪表系列：全中文智能涡街流量计、涡轮流量计；显示仪表系列：智能LED数字显示调节控制仪、智能LCD界面显示调节控制仪、光柱指示数字显示调节报警仪等。

Liuzhou some synthetic oilName: Lithium Grease, molybdenum disulfide, high temperature grease, high temperature electrical fat, kiln cars fat, high-temperature extrusion fat, Jinniu grease, fat-specific aluminum alloy, high temperature high fat, high-temperatureanti-stick agent, high temperature chain oil, high and low temperature bearing grease, aviation fat, lowering device fat, thimble fat, very low-temperature extreme pressure grease, high-low temperature grease, piston ring seal fat, calcium complex, lithium complex, aluminum composite, the damping oil, urea-based grease, oil roller, plastic gear grease, dust, grease, toys, fat, fat gyro motors, conductive grease, thermal conductivity Silicone Grease, anti - irradiated lipid, dimethyl silicone oil, health-grade silicone, high vacuum silicone oil viscosity standard oil, instrument oil, top oil, optical silicone damping oil, bearing oil, watches oil, fuse oil, rust-proof resin, cathode electrophoresis fat, fat loose screws, wire rope surface fat, Ma core fat, anti-rust oil, insulating resin 73, from the type agent, release agent, insulation resins, sealants, insulating oil, high-phenyl silicone oil , photocopiers oil, pump oil, thread grease, gear grease, bit grease, allow water-based grease, gear lubricant spray, turbine vortex rod oil, gear oil, gear oil capacity waterborne, rail oil, Silicone Grease, oil pump, turbo molecular pump oil, synthetic compressor oil, refrigeration oil, heat-conducting oil, hydraulic oil, hydraulic oil temperature, anti-burning hydraulic oil, cycle oil, gear oil, transmission fluid, damping fluid, body Annex fat, fat joints, antifreeze, dedicated reducer fat, calcium-based resin, white resin specialty, food grease, Vaseline, cream-filled cables, the spread of oil, softening oil, white oil, knitting oil, split seed oil, spindle oil, sparks oil, diesel oil, quenching oil, forming oils,; Drawbead oil, cutting oil, aluminum rolling liquid, titanium cutting oil, oil tapping oil watchcase extrusion, die-casting pieces of cutting oils, stamping oils, shut up oil, Tensile oil, extreme pressure cutting oil, cutting oil, grinding oil, shaving arrow oil, volatile oil punching, multi-function processing oil, cable oil,; nut processing oil, cutting oil, platen oil, cleaning agents, micro-emulsified oil, copper and aluminum microemulsifying oil, emulsified oil, emulsified oil cooling, multi-effect emulsification oil, water-based tensile oil, synthetic water-based cutting fluid, copper wire drawing oils, extreme pressure emulsified oil, anti-rust Emulsified oil, rolling oil emulsion, thread cutting oil,; water-based cleaning agent, aqueous forming agents, mold release liquid aluminum alloy, magnesium alloy, such as mold release liquid.

特士龙部分合成油品名称：锂基脂、二硫化钼、高温润滑脂、高温电机脂、窑车脂、高温挤压脂、金牛油脂、铝合金专用脂、高温高速脂、高温丝扣防粘滑剂、高温链条油、高低温轴承脂、航空脂、降器脂、顶针脂、极低温极压脂、高低温脂、活塞环密封脂、复合钙、复合锂、复合铝、阻尼油、脲基脂、轧辊油、塑胶齿轮脂、防尘脂、玩具脂、陀螺马达脂、导电脂、导热硅脂、抗辐照脂、二甲基硅油、卫生级硅油、高真空硅油、粘度标准油、仪表油、陀螺油、光学硅油、阻尼油、轴承油、手表油、引信油、防锈脂、阴极电泳脂、螺丝松动脂、钢丝绳表面脂、麻芯脂、防锈油、绝缘脂73、离型剂、脱模剂、绝缘树脂、密封剂、绝缘油、高苯基硅油、复印机油、电泵油、螺纹脂、齿轮脂、钻头脂、容水性润滑脂、齿轮喷雾润滑剂、涡轮涡杆油、齿轮油、容水性齿轮油、导轨油、硅脂、真空泵油、涡轮分子泵油、合成压缩机油、冷冻机油、导热油、液压油、宽温液压油、抗燃液压油、循环油、排档油、传动液、减震液、车身附件脂、万向节脂、防冻液、减速机专用脂、钙基脂、白色特种脂、食品脂、凡士林、电缆填充膏、扩散油、软化油、白油、针织机油、绽子油、主轴油、火花机油、柴油机油、淬火油、成型油、拉延油、切削油、铝轧制液、钛合金切削油、表壳挤压油攻牙油、压铸件切削油、冲压油、收口油、拉伸油、极压切削油、精密切削油、研磨油、剃矢油、挥发冲剪油、多功能加工油、拉线油、螺帽加工油、快削油、压板油、清洗剂、微乳化油、铜铝微乳化油、乳化油、冷却乳化油、多效乳化油、水性拉伸油、合成水性切削液、铜拉丝油、极压乳化油、防锈乳化油、轧制乳化油、线切割油、水性清洗剂、水性成型剂、铝合金脱模液、镁合金脱模液等。

Trans-Blot Plus Cell 170-3990 Trans-Blot Plus Cell With Plate Electrodes and Super Cooling Coil, includes 3 gel holder cassettes, buffer tank, lid with power cables, 6 fiber pads, 1 pack blot absorbent filter paper (26.5 x 28 cm, 30 sheets), roller, stirbar 170-3991 Trans-Blot Plus Cell and PowerPac HC Power Supply Accessories 170-3994 Trans-Blot Plus Gel/Cassette Assembly Tray 170-3995 Fiber Pads, 27 x 28.5 cm, 2 170-3996 Blot Absorbent Filter Paper, 26.5 x 28 cm, 60 sheets 170-3997 Stirbar 170-3998 Trans-Blot Plus Roller, 6 wide 170-39 Trans-Blot Plus Gel Holder Cassette With Clamps 170-4990 Trans-Blot Plus Super Cooling Coil 170-4991 Trans-Blot Plus Platinum Anode Plate Electrode 170-4992 Trans-Blot Plus Stainless-Steel Cathode Plate Electrode 170-4995 Trans-Blot Plus Cell Buffer Tank 170-4996 Trans-Blot Plus Cell Lid With Cables 170-4997 Gel Holder Cassette Clamps, for Trans-Blot Plus cell, set of 3 9 规格:说明:运用Mini-PROTEAN II 多道筛选仪,可以快速有效地筛选 40 种不同的抗体或血清,无需把western

转印条件可调，能在很大的分子量跨度上获得最佳转印效果*耐用的板式电极能产生强大而均一的电场，而且电极在槽内的位置灵活可换；不论运行1、2 或3 个转印夹，电极间距可调节到最小以获得最大的场强和转印效率*凝胶支架转印夹保证整个凝胶与印迹膜表面的均衡接触*转印夹的铰链设计能防止凝胶滑动，便于转印夹组装*颜色标记的转印夹及电极板确保在转印槽内的正确定向*特级冷却芯和冷却水循环器进行温度调节―可理想地用于天然酶或高强度转印，或用于延长转印时间时减少缓冲液的损耗*可选择的组装盘是凝胶三明治和转印夹组装的理想选择 Trans-Blot Plus 转印槽能从单向和双向大格式凝胶上均一、高效地转移蛋白―配合使用新推出的 PowerPac HC 电源，多数蛋白都可在1530 分钟内完成转印。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。