多核细胞的

On the morphological differentiation,by inducing of fetal calf serum,it wasshown that the cytotrophoblast cells underwent aggregation first,and the adjacentcytoplasm membranes became tightly contacted through desomosomes andcytoskeleton.Then the adjacent cytoplasm membranes disintegrated fragmentallyto form fusion pore-like structure.Finally the multinuclear cells formed and inwhich the intercellular desmosomes disappeared.Immunocytochemical stainingfurther demonstrated that the formation of syncytium was through cell membranefusion rather than the amitosis process.

细胞形态分化的研究结果表明：早孕胎盘来源的细胞滋养层细胞在血清诱导下，首先发生聚集，并通过桥粒和细胞骨架使相邻的细胞膜紧密接触；之后细胞膜发生片段断裂，形成融合孔样结构；细胞间桥粒消失，最终形成多核的合体细胞；免疫细胞化学分析进一步证实合体化是通过细胞膜融合而非无丝分裂（amitosis即细胞核分裂而细胞质不分裂）实现的。

Myoblast that isabundent and easy to get can fuse with the local myoblast to formpolynuclemyoblast and live in myofiber after transplantation.

成肌细胞数量丰富，易于获取，可以通过注射进行大规模细胞移植，可与宿主的肌肉细胞融合形成多核肌细胞，并可以在肌纤维中存活。

The relationship between the proterozoic Era and explosive evolution of the life on the Cambrian period was preliminary explored by an Ecological and Evolutionary approach in this paper.

距今38亿年前地球最早生命诞生以来，至元古代早期第一个真核细胞开始出现；从无性分裂或出芽繁殖的原核生物到有性繁殖的真核单细胞生物以及从单细胞的原生动物到真核多细胞的后生动物的进化，无疑是生命进化过程中非常重要的事件。

Except the incomplete maturation of spermatid nuclear and oocyte activation, idendification of a live spermatid is a pivotal procedure. It is difficult to distinguish round spermatids from other round cells such as spermatocytes, monocytes, polymorphonuclear leukocytes and so on.

除精子细胞的核蛋白不完全成熟及卵子激活不足等因素外，如何正确选择存活精子细胞是个难题，如圆形精子细胞与精母细胞、单核细胞和多形核白细胞等其他圆形细胞的区分就比较困难。

FUNGI Fungi are almost entirely multicellular, heterotrophic (deriving their energy from another organism, whether alive or dead), and usually having some cells with two nuclei multinucleate, as opposed to the more common one, or uninucleate per cell.

FUNGI Fungi是几乎完全地多细胞性，异养(获得他们的能量从另一个有机体，是否活或死)和通常有与二个中坚力量的有些细胞多核，与更加共同一个相对或者单核每个细胞。

1,After transfected the mutated mtDNA of colorectal carcinoma,the mtDNA D-loop region of the transfected cells displays new mutation points.2,The external source pieces of the mutated mtDNA can integrate to nuclear genome after transfection.3,There's no differences in apoptosis between combinations after transfected the mutation of mtDNA in NIH3T3 and LST cells.4,The mutated mtDNA may affect the action mechanism of occurrence and development in colorectal carcinoma through affecting its mtDNA mutation or integrating exogenetic mtDNA to its nuclear which may cause the abnormal expression of oncogene or anti-oncogene.

（1）转染突变的大肠癌细胞mtDNA后转染细胞的mtDNA均可发生多处的突变位点。（2）通过转染后突变的外源性的mtDNA可以整合到核基因组内。（3）突变的mtDNA转染LST细胞及NIH3T3细胞后，不影响转染细胞的凋亡改变。（4）mtDNA的突变可能通过影响体细胞mtDNA的突变和通过外源性mtDNA在核内的整合从而影响癌基因或抑癌基因的表达异常，从而参与肿瘤的发生发展。线粒体DNA；D―环区；突变；质粒；pcDNA3.1；转染

Yantai Normal College,264025,ChinaABSTRACT Most epithelial nuclei of Nuttallia olivacea are elliptic in shape and the chromatin is filiform.Most nuclei of connective tissue are round and have Feulgen positive granules.

摘 要 紫彩血蛤的鳃上皮细胞核多为椭圆形，核内染色质呈丝状；结缔组织细胞的核多为圆形，部分核内有块状Feulgen阳性颗粒。

The cell cycle and apoptosis and the expression of P-pg, bcl-2, P53 in the MG63 and MG63/R cell lines were analyzed by flow cytometry. Results The resistance index of the MG63/R cells to cisplatin was 83.557±4.841. The cells also had resistance to doxorubicin, vincristin, methotrexate and cyclophosphamide. Disordered structure of the MG63/R cells was observed through microscopy. The cells appeared in triangle, polygon and polynucleation. The increase of granular endoplasmic reticulums and apophyses was observed through transmission electron microscopy. The proliferation ability of MG63/R increased significantly, with a low apoptosis index.

结果 经顺铂186d的诱导，建立了MG63/R，其对顺铂的耐药指数为83.557±4.841，对阿霉素、长春新碱、氨甲基蝶吟、环磷酰氨亦产生不同程度的交叉耐药；光镜观察可见MG63/R细胞排列不规则，形态呈三角形、多角形及多核现象；透射电镜显示MG63/R细胞表面突起增加，粗面内质网丰富；细胞周期分析显示细胞增殖能力明显增加而细胞凋亡明显降低；MG63/R细胞 P-pg、bcl-2阳性表达较MG63细胞明显增加，P53表达则明显降低。

Methods Fourteen mutated vpr fragments were selected from patients with HIV. Both eukaryotic expression vector pcDNA3.1 and PCR products were purified, double-cut by Hind Ⅲ and BamH Ⅰ, and the cut products were legated and transformed into competent cells JM109. The 14 reconstructed plasmids were transfected into Hela cells. Cells with pcDNA vpr-wt, pcDNA vpr-Fs and pcDNA3.1 blank cells, and without pcDNA3.1 cell were established.

以14个带有HIV-1vpr基因片段的PcD-NA3.1真核表达载体构建重组质粒，将其转染Hela细胞，并设立保守株vpr基因转染细胞、突变株vpr-FS基因转染细胞、空载体转染细胞和未转染细胞作为对照，经逆转录多聚酶链式反应检测目的基因转染成功后，Pi染色，用流式细胞仪检测被转染细胞的细胞周期分布和细胞凋亡率。

Complicated than procaryotes. These cells, exemplified by the yeast in Figure 4.1-3, have a membrane surrounding the cell which is similar in structure to the procaryote membrane.

真核细胞，具有真正的细胞核，其结构要比原核生物复杂的多，以图4.1-3中所示的酵母菌为例，和原核细胞一样真核细胞也具有一层细胞膜。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。