多核体

EpNPV-Bt preparation, a new biological pesticide which is a mixture of bacillus thuringiensis and Euproctis pseudoconspersa Strand nuclear polyhedrosis virus, is provided with the virtue of non-toxicity, non-residue and non-pollution.

茶毛虫病毒Bt混剂是茶毛虫核型多角体病毒与苏云金杆菌混配而成的生物农药，具有无毒、无残留、不污染环境等优点。

Using cytochalasin D to prevent global actin from forming filamentous structures, the production of Heliothis armigera nuclear polyhedrosis virus in Hz-AM1 cells grown in the medium containing 0.5μg/ml CD was completely inhibited while its production was reduced to 10〓 in the presence of 0.1μg/ml CD by plaque assay. However, Western blotting revealed that the actin quantity in infected host cells treated or untreated with CD was almost identical. Additionally, CD had no effect on the DNA synthesis of HaNPV.

Western blot表明细胞松弛素D并不影响受染细胞中肌动蛋白的含量，Dot blot表明病毒DNA的合成也没有受到影响，但电子显微镜超薄切片发现在0.5ug／mL细胞松弛素D中形成的病毒粒子形态与正常的有明显区别，认为细胞松弛素D抑制棉铃虫核型多角体病毒的增殖是因为抑制病毒的DNA和蛋白组装成完整有感染性的病毒粒子，即丝状肌动蛋白对病毒粒子的脱壳和DNA的复制、转录没有影响，但对病毒粒子的组装是必须的。

By using PCR method, a 1kb fragment was gained from Heliothis amigera nuclear polyhedrosis virus genome. The whole fragment was sequenced by dideoxided chain termination methods and a full open reading frame which encode 299 amino-acids was found. The molecular weight of the peptide is about 35 kilodalton.

用PCR方法从棉铃虫核多角体病毒基因组中扩增到了1kb的DNA片段，用双脱氧链终止银染测序法测定了该片段的全序列，发现它含有一个完整的开放阅读框，编码299个氨基酸残基的多肽，分子量约35KD。

In addition, baculovirus are noninfectious to vertebrates, and their promoters have been shown to be inactive in mammalian cells. Futhermore, the expression level of some recombinant proteins is up to 1000 mg/L. Finally, recombinant proteins are soluble and antigenically, immunogenically and functionally similar to their authentic counterparts. In this article, we used the transfer vector pBlueBac of AcNPV (Autographa californica Nuclear Polyhedrosis Virus) to construct the recombinant baculovirus AcNPV-GM-CSF by in vivo recombinantion.

本文中，我们首先利用苜蓿夜蛾核型多角体病毒带β-Galactosidase 基因标记的非融合蛋白基因转移载体pBlueBac，通过细胞内同源重组的方式将hGM-CSF基因成功地插入病毒AcNPV的基因组中。hGM-CSF基因编码信号肽和完整的氨基酸序列。hGM-CSF基因在感染重组病毒的草地夜蛾培养细胞Sf9中得到表达，感染后的Sf9细胞培养液能刺激人骨髓细胞在体外形成典型的集落，表达水平可达2.7〓CFU／ml。

The biocontrol efficiency of wild type virus and two in vitro reproductive strains (N81 and G) generated from LD-652Y cells were evaluated by feeding second instar larvae of Lymantria xylina with these virus.

本论文共分成两部份：第一部份为探讨黑角舞蛾核多角体病毒在生物防治上的杀虫效率，并且利用制剂配方来增强病毒杀及应用性。

Identification of granulomatous prostatitis should pay attention to the formation of acinar small tubular lesions and normal relations without change, often visible amyloid degeneration and multinucleated giant cells.

鉴别时应注意肉芽肿性前列腺炎的腺泡形成很少，病变与正常腺管的关系无改变，常可见退行性变的淀粉样体和多核巨细胞。

A new picorna-like virus was isolated from dead Evtropis oblique larvae deceased from NPV infection. Electron microscopic observations of purified virions were non-enveloped isometric particles with diameter of 26nm. The virions contain two capsid proteins: 31.5kDa and 28.8kDa, the amount of later is 2.5 times more than that of the former. Analysis of 3′terminal sequence of EoPV clone identified that it can encode RdRp and has eight conserved motifs. Homology analysis shows that it is closely related to Perina nuda picorna-like virus.

从核型多角体病毒感染致死的茶尺蠖幼虫尸体中分离到一株微小RNA病毒透射电镜观察纯化的病毒粒子为无囊膜、无表面特征、直径约26nm的球状颗粒。16%的SDS-PAGE显示它有两个分子量为31.5 kDa和28.8 kDa的衣壳蛋白，后者的含量是前者的2.5倍。3′端克隆序列分析表明EoPV基因组3′有poly尾，编码RNA聚合酶，含有微小RNA病毒RNA聚合酶的八个保守基序，同源性分析表明它与榕透翅毒蛾微小RNA病毒亲缘关系最近。

Analysis of mitochondrial DNA composition of the panda-rabit cloned embryos found that mitochondria from both panda somatic cells and rabbit ooplasm co-existed in early blastocyst, but mitochondria from rabbit ooplasm decreased and those from panda donor cells dominated in early fetuses after implantation.

结果显示，应用卵裂球电融合方法可以制作一系列的多倍体胚胎；小鼠囊胚的形成与胚胎中的细胞数目无直接关系，卵裂球的电融合和染色体数目的增加不会改变胚胎的发育进程；而胚胎细胞中的核／质比可能是控制胚胎囊胚化的一个重要因素。

This work is mainly concerned with molecular magnetism of polynuclear complexes and supramolecular crystal engineering.

本论文围绕多核配合物的分子磁学和超分子晶体工程这两个最为活跃的前沿研究领域，选用大环配体tacn（1，4，7-三氮杂环壬烷）和含有外延桥的双草胺。。。

The observation with histomorphology and phase contrast microscope: osteoclasts were large in size, multinucleus, foam-like cytoplasm with vacuole and pseudopodia, and movable. Acid Phosphatase reaction is positive.

经组织形态学、光镜和相差显微镜观察，破骨细胞具有胞体大，多核，胞浆泡沫状间有空泡，胞浆有伪足样突起，并可移动，酸性磷酸酶反应阳性。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷